| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,529,506 | C→T | A280T (GCG→ACG) | livJ ← | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,529,506 | 0 | C | T | 98.6% | 561.0 / ‑2.2 | 145 | A280T (GCG→ACG) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (87/56); total (87/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
TAGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAG > REL606/3529433‑3529578 | tAGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTtcgtcg < 2:5031721/76‑1 (MQ=255)tAGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTtcgtcg > 2:4887471/1‑76 (MQ=255) aGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCgg < 2:5736659/76‑1 (MQ=255) aGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCgg > 1:758979/1‑76 (MQ=255) aGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCgg > 1:3271876/1‑76 (MQ=255) aGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCgg < 2:3504214/76‑1 (MQ=255) ggtggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGa < 1:2289669/76‑1 (MQ=255) gtggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGaa < 2:6193935/76‑1 (MQ=255) gtggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGaa > 1:5391093/1‑76 (MQ=255) tggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAAc < 1:4786755/76‑1 (MQ=255) tggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAAc < 2:3801940/76‑1 (MQ=255) ggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAAcc < 1:4081186/76‑1 (MQ=255) ggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAAcc < 1:5558393/76‑1 (MQ=255) ggtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAAcc > 2:4342840/1‑76 (MQ=255) gtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCt > 2:1083245/1‑76 (MQ=255) gtCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCt > 1:6170364/1‑76 (MQ=255) tCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTg < 2:3651733/76‑1 (MQ=255) aGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATc < 2:4848517/76‑1 (MQ=255) aGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATc < 1:1390978/76‑1 (MQ=255) aCGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGt > 2:4349470/1‑76 (MQ=255) gAATGCGCCTCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAg < 2:1471276/76‑1 (MQ=255) gAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAg > 2:4162342/1‑76 (MQ=255) gAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAg < 1:5839233/76‑1 (MQ=255) gAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAg > 1:1077206/1‑76 (MQ=255) aaTGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGt < 1:1088421/76‑1 (MQ=255) aaTGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGt < 1:1997583/76‑1 (MQ=255) aTGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGtt > 1:5572261/1‑76 (MQ=255) gccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGttcttc < 1:1455623/76‑1 (MQ=255) gccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGttcttc > 1:5875903/1‑76 (MQ=255) ccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCg > 1:5776625/1‑76 (MQ=255) ccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCg < 2:3775576/76‑1 (MQ=255) ccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCg > 2:5837764/1‑76 (MQ=255) ccgcTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCg < 1:5549391/76‑1 (MQ=255) cTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCt < 1:4634680/76‑1 (MQ=255) cTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCt > 2:300681/1‑76 (MQ=255) tGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCttt > 1:4327506/1‑75 (MQ=255) tGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTg > 1:4208203/1‑76 (MQ=255) tGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTg > 1:671201/1‑76 (MQ=255) tGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTg > 1:591434/1‑76 (MQ=255) gggTGCTGGTTTTTCGCTTTGATCGCGTCAACGTTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCttct > 2:2176455/1‑74 (MQ=255) gTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTc > 2:2874285/1‑76 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCa > 2:2798227/1‑76 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCa < 1:5573251/76‑1 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCa < 1:3993707/76‑1 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCa < 1:1254442/76‑1 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCa > 2:402846/1‑76 (MQ=255) tCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGGCa > 2:4551697/1‑76 (MQ=255) ccTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCAc > 2:2422160/1‑76 (MQ=255) tGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACca < 1:3133271/76‑1 (MQ=255) gTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTTGTCAcccg > 1:3805740/1‑74 (MQ=255) gTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcag > 2:4683007/1‑76 (MQ=255) gTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcag < 1:3840897/76‑1 (MQ=255) gTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACaag > 1:1169194/1‑76 (MQ=255) ttttttCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagc < 2:6136581/76‑1 (MQ=255) ttttttCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagc < 1:3607982/76‑1 (MQ=255) ttttttCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcaac > 1:2472577/1‑74 (MQ=255) ttttCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagcag > 1:5919816/1‑76 (MQ=255) tttcCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagcag < 2:1972194/76‑1 (MQ=255) tttCGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTTGTGACCagcagc > 2:4416963/1‑76 (MQ=255) cGCTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCt < 1:3701273/76‑1 (MQ=255) cTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTc < 2:4518199/76‑1 (MQ=255) cTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTc > 1:4691455/1‑76 (MQ=255) cTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTc > 2:4508162/1‑76 (MQ=255) cTTTGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCCTc > 1:2022745/1‑76 (MQ=255) tttGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGACCtttc > 2:478265/1‑74 (MQ=255) ttGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCg < 1:3921069/76‑1 (MQ=255) ttGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCg < 1:6175554/76‑1 (MQ=255) ttGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCg > 1:4548394/1‑76 (MQ=255) tGATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGc > 2:1554333/1‑76 (MQ=255) gATCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCCTCCGCt > 1:3645048/1‑76 (MQ=255) aTCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGGCACCCGCAGCCCTTCCCCTg > 2:3358072/1‑76 (MQ=255) tCGCGTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGa < 2:5971457/76‑1 (MQ=255) cgcgTCAACAATGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCGCCAGCAGCCATTCACCTGAt > 2:2364322/1‑76 (MQ=255) acaaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCg < 2:6212000/76‑1 (MQ=255) caaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCCGCAGCCCTTCCGCTGATTCGCCCGc > 1:2800824/1‑76 (MQ=255) caaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGc > 2:4295113/1‑76 (MQ=255) aaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTTGGCACCAGCAGCCCTTCCGCTGATTCGCCCCCa > 2:159753/1‑76 (MQ=255) aaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGcc > 1:3487802/1‑75 (MQ=255) aaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCa > 1:3037697/1‑76 (MQ=255) aaTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCCCa > 2:1733105/1‑76 (MQ=255) aTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCaa < 2:2394864/76‑1 (MQ=255) aTGGGTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGGCACCAGCAGCCCTTCCGCTGATTCGCCCGcca > 2:565702/1‑74 (MQ=255) gggTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATg > 2:787725/1‑76 (MQ=255) gTTTGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGtt > 2:1878810/1‑76 (MQ=255) tttGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTa > 1:6009191/1‑76 (MQ=255) tttGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTa > 1:1371926/1‑76 (MQ=255) ttGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAg > 1:6020326/1‑76 (MQ=255) ttGTTCGTCAGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAg < 1:6239414/76‑1 (MQ=255) ttGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAg < 2:6085731/76‑1 (MQ=255) tGTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGa > 2:403345/1‑76 (MQ=255) tGTTCGTCGGAACCTGATCGTAGTTCTTCGCCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGa < 2:3205503/76‑1 (MQ=255) gTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGAc > 2:5637856/1‑76 (MQ=255) gTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGAc < 2:1052116/76‑1 (MQ=255) gTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGAc > 2:2309255/1‑76 (MQ=255) gTTCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCCCAATGTTAGAc > 1:2448842/1‑76 (MQ=255) ttCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACa > 2:533402/1‑76 (MQ=255) ttCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACa < 1:2965406/76‑1 (MQ=255) ttCGTCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACa < 1:4866976/76‑1 (MQ=255) tcgtcgGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAg < 2:5262866/76‑1 (MQ=255) tcgtcgGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAg < 1:1554333/76‑1 (MQ=255) | TAGGTGGTCCAGACGAATGCGCCGCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAG > REL606/3529433‑3529578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |