| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,589,641 | G→A | E307K (GAA→AAA) | yhiU → | multidrug resistance efflux transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,589,641 | 0 | G | A | 98.5% | 794.5 / ‑0.2 | 200 | E307K (GAA→AAA) | yhiU | multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (111/86); total (113/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
Only 100 of 209 total aligned reads displayed.
CTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACG > REL606/3589568‑3589714 | cTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGaaa < 2:4122247/76‑1 (MQ=255) tgctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAgg > 1:943311/1‑76 (MQ=255) tgctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAgg > 1:1823042/1‑76 (MQ=255) gctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGc > 1:1593307/1‑76 (MQ=255) ctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCg > 2:4948217/1‑76 (MQ=255) tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 2:4528355/1‑76 (MQ=255) tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 2:1105294/1‑76 (MQ=255) tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 1:5940331/1‑76 (MQ=255) tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt < 1:5479965/76‑1 (MQ=255) tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 1:4505925/1‑76 (MQ=255) gcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACCGGTGCCGCAGAAAGGCGTc > 2:3722261/1‑76 (MQ=255) ccTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCa < 1:4134064/76‑1 (MQ=255) tGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCAcc < 2:1593307/76‑1 (MQ=255) ggCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCAccc < 2:6000944/76‑1 (MQ=255) gaaTGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCca < 2:5264148/74‑1 (MQ=255) cATTTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcac > 2:3434310/1‑76 (MQ=255) cATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcac > 1:4571284/1‑76 (MQ=255) aTGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcaca > 2:5239375/1‑76 (MQ=255) aTGTACGTCACGGCATTAGTGGATGAAGGTAGCCCCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcaca < 1:3884676/76‑1 (MQ=255) tGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACaa > 2:2103253/1‑76 (MQ=255) gTACGTCACGGCTTTAGGGGAGGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAAc < 2:3280322/76‑1 (MQ=255) gTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAAc > 2:2275195/1‑76 (MQ=255) tgcGTCACGGCATTAGGGGATAAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACg < 2:3519776/74‑1 (MQ=255) tACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACg > 2:4800325/1‑76 (MQ=255) aCGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCCCCCACAACGc > 1:1343239/1‑76 (MQ=255) cGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGcc > 2:1313106/1‑76 (MQ=255) gTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGccc > 1:5519103/1‑76 (MQ=255) tCACGGCTTTAGTGGAGGAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa < 2:2505793/76‑1 (MQ=255) tCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa > 2:1911979/1‑76 (MQ=255) tCACGGCATTAGGGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa < 1:1048290/76‑1 (MQ=255) cACGGCGTTAGGGGATAAAGGTAACCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAg < 1:4638436/76‑1 (MQ=255) cACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAg < 1:6200931/76‑1 (MQ=255) aCGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAgg > 1:3421234/1‑76 (MQ=255) cGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAggg < 2:4597730/76‑1 (MQ=255) ggCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAgggg > 2:5078290/1‑75 (MQ=255) gCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTa > 2:4282752/1‑76 (MQ=255) gCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGACCAGGGTa > 1:3918330/1‑76 (MQ=255) gCATTAGTGGAGGAAGTTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTa < 1:1747909/76‑1 (MQ=255) gCATAAGTGGATGAAGTTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGGCACCCACAACGCCCAGGGTa < 2:2600852/76‑1 (MQ=255) cATTGGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTaa < 1:3557023/76‑1 (MQ=255) aTTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTaaa > 2:4023798/1‑76 (MQ=255) ttAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAg < 2:3107005/76‑1 (MQ=255) ttAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAg > 1:3146076/1‑76 (MQ=255) tAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGc > 1:1364396/1‑76 (MQ=255) tAGTGGATAAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGc < 1:4820109/76‑1 (MQ=255) aGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCa > 2:2837495/1‑76 (MQ=255) gTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCaa > 1:3119585/1‑76 (MQ=255) ggATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACg < 2:3050399/76‑1 (MQ=255) gATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACgg > 1:2234739/1‑76 (MQ=255) gATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACgg > 1:6073575/1‑76 (MQ=255) aTGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgc > 2:3369623/1‑76 (MQ=255) aTGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgc > 1:1645027/1‑76 (MQ=255) tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACTCACAACGCCCAGGGTAAAGCAACGgcg > 2:5304533/1‑76 (MQ=255) tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgcg > 1:5989949/1‑76 (MQ=255) tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgcg > 2:4891587/1‑76 (MQ=255) agggTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCt < 2:3340405/74‑1 (MQ=255) aaGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAACAACGGCGCt > 1:5112553/1‑76 (MQ=255) aGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTc < 2:5731784/76‑1 (MQ=255) gTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCAt > 2:1534548/1‑76 (MQ=255) aGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTc > 1:6142640/1‑76 (MQ=255) aGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTc > 1:5162908/1‑76 (MQ=255) gccgccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCt < 1:2933074/76‑1 (MQ=255) cgccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTgg < 1:155212/76‑1 (MQ=255) ccAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:722588/76‑1 (MQ=255) ccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:2444411/76‑1 (MQ=255) ccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:6157845/76‑1 (MQ=255) gAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATaaa > 2:121958/1‑76 (MQ=255) aaTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAg < 1:5336485/76‑1 (MQ=255) aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa < 1:663002/76‑1 (MQ=255) aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa > 1:1402877/1‑76 (MQ=255) aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa > 1:278110/1‑76 (MQ=255) tGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGAc < 2:4842923/76‑1 (MQ=255) tGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGAc > 1:6307936/1‑76 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 2:5950156/76‑1 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:1197645/76‑1 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg > 2:6282400/1‑76 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 2:4109453/76‑1 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:1722153/76‑1 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg > 1:2424138/1‑76 (MQ=255) gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:3369623/76‑1 (MQ=255) tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 2:195825/1‑76 (MQ=255) tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 1:2112915/1‑76 (MQ=255) tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 1:2975700/1‑76 (MQ=255) ttACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATg > 2:4331945/1‑76 (MQ=255) ttACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATg < 2:1324971/76‑1 (MQ=255) tACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGt > 2:1838511/1‑76 (MQ=255) cTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCg < 2:5422793/76‑1 (MQ=255) tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt < 2:2200766/76‑1 (MQ=255) tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt > 2:6213853/1‑76 (MQ=255) tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt > 1:4195217/1‑76 (MQ=255) ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 2:2255398/76‑1 (MQ=255) ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 1:395980/76‑1 (MQ=255) ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 2:370323/76‑1 (MQ=255) gTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGc > 1:3203774/1‑76 (MQ=255) ccGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGc > 2:6206305/1‑76 (MQ=255) ccGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGc < 1:5806647/76‑1 (MQ=255) cGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCt > 1:3277432/1‑76 (MQ=255) cGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCt < 1:5256641/76‑1 (MQ=255) cAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTAc > 2:893811/1‑76 (MQ=255) aGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTAcg > 1:567676/1‑76 (MQ=255) | CTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACG > REL606/3589568‑3589714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |