BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,944,574	C→T	noncoding (40/77 nt)	proM →	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,944,574	0	C	T	98.7%	608.9 / ‑2.3	158	noncoding (40/77 nt)	proM	tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (69/87); total (71/87)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 159 total aligned reads displayed.

AAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTT  >  REL606/3944501‑3944646

aaGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCa                                                                        <  1:1154693/76‑1 (MQ=255)

 aGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAg                                                                       >  2:872141/1‑76 (MQ=255)

 aGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAg                                                                       >  2:1933655/1‑76 (MQ=255)

 aGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGAGCAGCTTGGTAGCGCAACTGGTTTGGGATCAg                                                                       <  1:2897535/76‑1 (MQ=255)

  gAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGt                                                                      <  2:6040682/76‑1 (MQ=255)

   aaTTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTg                                                                     >  2:5939046/1‑76 (MQ=255)

    aTTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTgg                                                                    >  1:2936020/1‑76 (MQ=255)

    aTTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTgg                                                                    <  1:4597030/76‑1 (MQ=255)

    aTTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTgg                                                                    <  2:3065452/76‑1 (MQ=255)

     ttGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTggg                                                                   >  2:693897/1‑76 (MQ=255)

     ttGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTggg                                                                   >  1:5191904/1‑76 (MQ=255)

      tGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGt                                                                  >  1:2127645/1‑76 (MQ=255)

        aaCTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCg                                                                <  2:5542194/76‑1 (MQ=255)

        aaCTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCg                                                                <  1:422651/76‑1 (MQ=255)

         aCTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCgg                                                               <  1:2807425/76‑1 (MQ=255)

           tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCGACTGGTTTGGGATCAGTGGGTCGGAg                                                             <  1:5691039/76‑1 (MQ=255)

           tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAg                                                             <  1:1213296/76‑1 (MQ=255)

           tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAg                                                             >  1:3904611/1‑76 (MQ=255)

            aaaaaTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCCGAgg                                                            >  2:1774801/1‑76 (MQ=255)

                aTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCg                                                        >  1:2820750/1‑76 (MQ=255)

                aTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCg                                                        <  2:4583873/76‑1 (MQ=255)

                  tCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGaa                                                      >  1:6184274/1‑76 (MQ=255)

                  tCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGaa                                                      <  1:933129/76‑1 (MQ=255)

                  tCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGaa                                                      >  2:1454227/1‑76 (MQ=255)

                  tCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGaa                                                      <  1:105191/76‑1 (MQ=255)

                   cAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAAt                                                     >  2:5997149/1‑76 (MQ=255)

                    aaaaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATc                                                    >  2:4384249/1‑76 (MQ=255)

                     aaaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATcc                                                   >  2:1747326/1‑76 (MQ=255)

                     aaaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATcc                                                   >  2:1864213/1‑76 (MQ=255)

                      aaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCct                                                  <  1:560122/76‑1 (MQ=255)

                         gCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCctctc                                               <  1:4600988/76‑1 (MQ=255)

                         gCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCctctc                                               <  2:5855541/76‑1 (MQ=255)

                           aGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGGGGGTCGAATCCtctctc                                             >  1:4777475/1‑76 (MQ=255)

                           aGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCtctctc                                             <  1:2742579/76‑1 (MQ=255)

                           aGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCtctctc                                             <  1:324647/76‑1 (MQ=255)

                           aGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGGTCGAATCCtctctc                                             >  2:4467869/1‑76 (MQ=255)

                             tATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGc                                           <  1:6016914/76‑1 (MQ=255)

                             tATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGc                                           >  1:5960688/1‑76 (MQ=255)

                             tATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGGTCGAATCCTCTCTCGc                                           >  1:341863/1‑76 (MQ=255)

                             tATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGGTCGAATCCTCTCTCGc                                           >  1:5839907/1‑76 (MQ=255)

                              aTTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGATCGGAGGTTCGAATCCTCTCTCGcc                                          >  2:2983740/1‑76 (MQ=255)

                                ttCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGGGGGTCGAATCCTCTCTCGCCGa                                        >  1:5131666/1‑76 (MQ=255)

                                ttCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCgc                                        >  2:3927998/1‑75 (MQ=255)

                                 tCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc                                       <  2:4332224/76‑1 (MQ=255)

                                 tCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGGTCGAAATCTCTCTTGCCGAc                                       >  1:1823841/1‑76 (MQ=255)

                                  cGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAcc                                      <  1:2531239/76‑1 (MQ=255)

                                  cGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAcc                                      <  1:1114746/76‑1 (MQ=255)

                                     cGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAt                                   >  2:851242/1‑76 (MQ=255)

                                      gAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtt                                  <  2:4269578/76‑1 (MQ=255)

                                       aGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAttt                                 <  1:5659708/76‑1 (MQ=255)

                                       aGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAttt                                 >  1:1800273/1‑76 (MQ=255)

                                       aGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAttt                                 >  1:6130244/1‑76 (MQ=255)

                                       aGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAttt                                 >  2:3760355/1‑76 (MQ=255)

                                        gTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtttt                                <  2:913042/76‑1 (MQ=255)

                                        gTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtttt                                >  1:5668823/1‑76 (MQ=255)

                                        gTAGCGCAGCTTCGTGGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAAtttt                                <  1:1634778/76‑1 (MQ=37)

                                          aGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGa                              >  1:3679702/1‑76 (MQ=255)

                                          aGCGCAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGa                              >  1:4812164/1‑76 (MQ=255)

                                            cgcAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAAc                            >  1:3508280/1‑76 (MQ=255)

                                             gcAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCCATTTTGAAcc                           >  1:1594237/1‑76 (MQ=255)

                                             gcAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAAcc                           <  1:2585628/76‑1 (MQ=255)

                                              cAGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAAccc                          <  2:4521677/76‑1 (MQ=255)

                                               aGCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAAcccc                         >  1:3751977/1‑76 (MQ=255)

                                                gCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCg                        <  1:1569767/76‑1 (MQ=255)

                                                gCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCg                        <  2:123515/76‑1 (MQ=255)

                                                gCTTGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCg                        >  2:3908958/1‑76 (MQ=255)

                                                  ttGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCt                      <  2:4440206/76‑1 (MQ=255)

                                                  ttGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCt                      <  2:2025704/76‑1 (MQ=255)

                                                   tGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCtt                     <  2:6300032/76‑1 (MQ=255)

                                                   tGGTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCtt                     >  1:4316368/1‑76 (MQ=255)

                                                     gTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcg                   <  1:1986995/76‑1 (MQ=255)

                                                     gTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcg                   <  1:2321901/76‑1 (MQ=255)

                                                     gTAGCGCAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcg                   <  1:2974308/76‑1 (MQ=255)

                                                         cgcAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcggcgg               >  1:600186/1‑76 (MQ=255)

                                                         cgcAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcggcgg               <  2:4972501/76‑1 (MQ=255)

                                                         cgcAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTcggcgg               >  2:2156431/1‑76 (MQ=255)

                                                          gcAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCggg              <  2:3410950/76‑1 (MQ=255)

                                                          gcAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCggg              <  1:986770/76‑1 (MQ=255)

                                                           cAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCgggg             >  1:4259339/1‑76 (MQ=255)

                                                           cAACTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCgggg             <  2:495969/76‑1 (MQ=255)

                                                            aaCTGGTTTGGGATCAGTGGGTCGTAGCTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGt            <  2:1493846/76‑1 (MQ=38)

                                                             aCTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCgggggg           >  2:5374228/1‑74 (MQ=255)

                                                             aCTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtt           <  2:5167433/76‑1 (MQ=255)

                                                             aCTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtt           <  1:756670/76‑1 (MQ=255)

                                                             aCTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtt           <  2:5904472/76‑1 (MQ=255)

                                                             aCTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtt           <  2:3281707/76‑1 (MQ=255)

                                                              cTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttt          <  2:2521904/76‑1 (MQ=255)

                                                              cTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttt          <  2:5739912/76‑1 (MQ=255)

                                                              cTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttt          <  1:795295/76‑1 (MQ=255)

                                                              cTGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttt          <  2:4295400/76‑1 (MQ=255)

                                                               tGGTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtttt         <  1:5482059/76‑1 (MQ=255)

                                                                ggTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttttt        <  2:4786689/76‑1 (MQ=255)

                                                                ggTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttttt        <  2:5615589/76‑1 (MQ=255)

                                                                ggTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttttt        <  2:4026749/76‑1 (MQ=255)

                                                                ggTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGttttt        <  2:702898/76‑1 (MQ=255)

                                                                 gTTTGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtttttt       <  2:3553536/76‑1 (MQ=255)

                                                                  tttGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTg      <  2:234203/76‑1 (MQ=255)

                                                                    tGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGtt    <  1:384284/76‑1 (MQ=255)

                                                                    tGGGATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGtttttttt    >  1:783058/1‑76 (MQ=37)

                                                                      ggATCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGtttt  <  1:1961750/76‑1 (MQ=255)

AAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTT  >  REL606/3944501‑3944646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`