| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,985,084 | A→G | V437A (GTA→GCA) | ECB_03714 ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,985,084 | 0 | A | G | 98.8% | 695.1 / ‑3.1 | 170 | V437A (GTA→GCA) | ECB_03714 | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (55/113); total (55/115) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
Only 100 of 203 total aligned reads displayed.
TTATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCG > REL606/3985012‑3985156 | ttATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCa < 2:3460812/76‑1 (MQ=255)ttATTCATGTCGATGTGTGAAACTTGGTCCAGCAATTCGATTGCAGCGGTAGTGAACGCGCCATAACCAAGTGCCa < 2:5052790/76‑1 (MQ=255) tATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAg < 2:5941885/76‑1 (MQ=255) aTTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAgg < 2:4102295/76‑1 (MQ=255) cATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAAACAAGTGGCAGGTCg > 1:4029097/1‑76 (MQ=255) gTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGGGCCCTAAACACGGGCCAGGGCGaaa > 1:4946696/1‑75 (MQ=255) tCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGGGTAGTGGGAGGGCCCCAAACAAGTGCCAGGGCGGA‑Ta > 1:6147279/1‑76 (MQ=255) tCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Ta < 1:3032444/76‑1 (MQ=255) cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Taa < 1:1175673/76‑1 (MQ=255) cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Taa < 1:265658/76‑1 (MQ=255) cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGGGGAAGGGCCCACAACAAATGCCAGGGCGAA‑Taa > 2:2283232/1‑76 (MQ=255) gATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAac < 2:328238/76‑1 (MQ=255) gATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑Taat < 2:1444006/76‑2 (MQ=255) aTGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAaca < 1:626917/76‑1 (MQ=255) aTGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAaca < 1:5837311/76‑1 (MQ=255) tgtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAacac < 2:747379/76‑1 (MQ=255) tgtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGGGCCCTAACCAAGTGCCCGGGCGAA‑AAacac > 2:4342198/1‑76 (MQ=255) gtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacg < 1:5622529/76‑1 (MQ=255) gtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGGGGAAGGGCCATAAACAAGGTCCCGGGCGAACTAacaa > 2:4580423/1‑75 (MQ=255) gtgAAAGTTTGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgac < 1:4990478/76‑1 (MQ=255) gtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGCGCCCTAACCAAATGCCAGGGCGGA‑TAACaagac > 1:1854283/1‑76 (MQ=255) tgAAAGTTGGTCCAGTAATTCGCTGGCAGGGGTAGTGGGAGCGCCATAAACCAGTGCCCGGGCGAA‑TAACacgccg > 2:4551672/1‑76 (MQ=255) tgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgacg < 1:1075677/76‑1 (MQ=255) tgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgacg < 2:1625960/76‑1 (MQ=255) gAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACgg < 2:4299778/76‑1 (MQ=255) aGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGGGCCCTAAACAAGGGCCAGGGCGGA‑TAAAAAGACGGCAg > 1:4091392/1‑76 (MQ=255) gTTGGTCCAGTAATTCGCTGGCAGGGGGAGTGGAAGGGCCATAAACAAGTGCCACGTCGAA‑AAACACAACGGcggg > 2:5222280/1‑73 (MQ=255) gTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCCTAAACAAGAGCCAGGGCGAA‑TAACAAGACGGCAgg > 1:3740339/1‑75 (MQ=255) ttGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtg < 2:5759125/76‑1 (MQ=255) tGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtgt < 2:134542/76‑1 (MQ=255) tGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtgt < 1:1057267/76‑1 (MQ=255) tCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGtgtgtg < 1:3515683/76‑1 (MQ=255) ccAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGa < 2:141044/76‑1 (MQ=255) ccAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGa < 1:4311379/76‑1 (MQ=255) cAGTAATTCGCTGGCAGGCGTAGTGGAAGGGCCATAAACAAAGGCCCGGGCGGA‑TAACACGACGGCCGggtgtggg > 2:4486189/1‑74 (MQ=255) cAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAg < 1:248960/76‑1 (MQ=255) gTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTc < 1:1417151/76‑1 (MQ=255) gTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTc < 1:1496546/76‑1 (MQ=255) aTTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGtt < 2:4240231/76‑1 (MQ=255) aTTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGtt < 2:3989784/76‑1 (MQ=255) ttCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTg < 1:4567026/76‑1 (MQ=255) tCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGa < 2:1045920/76‑1 (MQ=255) gCTGGTAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGt < 2:5539736/76‑1 (MQ=255) gCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGt < 1:3636851/76‑1 (MQ=255) ggCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg < 2:338494/76‑1 (MQ=255) ggCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg < 2:2871592/76‑1 (MQ=255) ggCAGGAGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg < 2:94244/76‑1 (MQ=255) gCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGt < 2:2991222/76‑1 (MQ=255) cAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGtt < 1:12265/76‑1 (MQ=255) cAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGtt < 1:6036356/76‑1 (MQ=255) aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg < 1:3598235/76‑1 (MQ=255) aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg < 1:5810897/76‑1 (MQ=255) aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg < 1:5555650/76‑1 (MQ=255) gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg < 1:907874/76‑1 (MQ=255) gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg > 1:5043950/1‑76 (MQ=255) gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg < 2:3936936/76‑1 (MQ=255) gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa < 1:5244110/76‑1 (MQ=255) gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa > 1:2460810/1‑76 (MQ=255) gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa > 2:1711099/1‑76 (MQ=255) tAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAg < 2:5926701/76‑1 (MQ=255) aGTGGAAGCGCCCTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGAGGGTGAGTCGTTGAGTTCGTTTAGCAGa > 2:2067913/1‑76 (MQ=255) aGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGa > 1:5519469/1‑76 (MQ=255) aGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGa < 1:2599568/76‑1 (MQ=255) gTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGAc < 2:5657473/76‑1 (MQ=255) gTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGAc < 2:1153007/76‑1 (MQ=255) tGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACg > 1:2520962/1‑76 (MQ=255) ggAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGt > 2:5667738/1‑76 (MQ=255) ggAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGt > 1:4093779/1‑76 (MQ=255) gAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGtt < 2:6155877/76‑1 (MQ=255) aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg > 1:1136403/1‑76 (MQ=255) aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg < 1:1821196/76‑1 (MQ=255) aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg < 1:4864829/76‑1 (MQ=255) aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg > 1:2922270/1‑76 (MQ=255) cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt > 2:2557660/1‑76 (MQ=255) cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt < 1:3183257/76‑1 (MQ=255) cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt < 1:1755885/76‑1 (MQ=255) gcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTg < 2:2692966/76‑1 (MQ=255) ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc < 1:517151/76‑1 (MQ=255) ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc > 2:2788451/1‑76 (MQ=255) ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc > 2:1934711/1‑76 (MQ=255) cATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcg > 1:388504/1‑76 (MQ=255) cATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcg < 1:2211641/76‑1 (MQ=255) aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc < 2:4064436/76‑1 (MQ=255) aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc < 1:3976104/76‑1 (MQ=255) aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc > 1:2132123/1‑76 (MQ=255) aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc > 2:3853835/1‑76 (MQ=255) aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc > 2:5181595/1‑76 (MQ=255) tAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCa > 1:2334170/1‑76 (MQ=255) tAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCa > 1:5700584/1‑76 (MQ=255) ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag > 2:5469131/1‑76 (MQ=255) ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag < 2:5492025/76‑1 (MQ=255) ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag > 2:2445554/1‑76 (MQ=255) ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag < 1:5364231/76‑1 (MQ=255) cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt > 2:4853376/1‑76 (MQ=255) cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt > 2:3233076/1‑76 (MQ=255) cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt < 2:6282677/76‑1 (MQ=255) aaGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTc < 1:3117860/76‑1 (MQ=255) aaGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTc > 1:1646511/1‑76 (MQ=255) aGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCg > 1:2087170/1‑76 (MQ=255) aGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCg > 1:5956289/1‑76 (MQ=255) | TTATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCG > REL606/3985012‑3985156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |