BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,164,097	C→T	P1081L (CCG→CTG)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,164,097	0	C	T	100.0%	751.6 / NA	184	P1081L (CCG→CTG)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (102/82); total (102/82)

Only 100 of 187 total aligned reads displayed.

AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTC  >  REL606/4164024‑4164169

aaaCGCCGTATCCAGCCTGGTGACAAGATGACAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGa                                                                        >  2:3339822/1‑76 (MQ=255)

  aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATc                                                                      <  2:2383472/76‑1 (MQ=255)

   cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCg                                                                     >  1:420931/1‑76 (MQ=255)

   cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCg                                                                     <  1:5983447/76‑1 (MQ=255)

     ccGTACCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGaa                                                                   <  1:3814944/76‑1 (MQ=255)

      cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAg                                                                  >  1:4869360/1‑76 (MQ=255)

      cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAg                                                                  >  2:1144863/1‑76 (MQ=255)

       gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCTGATCGAAGa                                                                 >  2:3740650/1‑76 (MQ=255)

        tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGat                                                                <  2:3649654/76‑1 (MQ=255)

        tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGat                                                                >  1:2562883/1‑76 (MQ=255)

         aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGata                                                               <  2:5718823/76‑1 (MQ=255)

         aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGata                                                               <  2:5252570/76‑1 (MQ=255)

          tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGatat                                                              <  2:3979827/76‑1 (MQ=255)

          tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGatat                                                              <  1:1357536/76‑1 (MQ=255)

           ccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATg                                                             <  2:5316702/76‑1 (MQ=255)

           ccAGCCGGGTGACAAGGTGGCAGGTCGTCACTGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATg                                                             <  2:5316704/76‑1 (MQ=255)

             aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGcc                                                           <  2:4833506/76‑1 (MQ=255)

             aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGcc                                                           <  2:1095308/76‑1 (MQ=255)

             aGCCTGGTGACAAGATGGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGcc                                                           >  1:3247413/1‑76 (MQ=255)

               ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCtt                                                         <  2:3280451/76‑1 (MQ=255)

               ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCtt                                                         <  1:744759/76‑1 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        <  1:2793880/76‑1 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        >  1:3134448/1‑76 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        >  1:5025844/1‑76 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        <  2:1614388/76‑1 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        <  1:5740135/76‑1 (MQ=255)

                cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGGAATTTCTAAGATCAACCTGATCGAAGATATGCCTTa                                                        >  2:2202961/1‑76 (MQ=255)

                   gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGa                                                     >  2:3187403/1‑76 (MQ=255)

                    tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGAt                                                    >  1:2193613/1‑76 (MQ=255)

                     gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATg                                                   <  2:1361805/76‑1 (MQ=255)

                     gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATg                                                   >  2:5453873/1‑76 (MQ=255)

                      aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGa                                                  >  1:5846986/1‑76 (MQ=255)

                        aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGaaa                                                <  1:3167583/76‑1 (MQ=255)

                        aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGaaa                                                >  1:46428/1‑76 (MQ=255)

                          gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAAc                                              >  2:4234600/1‑76 (MQ=255)

                            tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACgg                                            <  2:3082642/76‑1 (MQ=255)

                             ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGt                                           <  1:6141617/76‑1 (MQ=255)

                             ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGt                                           <  2:5946901/76‑1 (MQ=255)

                             ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGt                                           >  1:5578899/1‑76 (MQ=255)

                              gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTa                                          >  1:2495821/1‑76 (MQ=255)

                              gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACCATGAAAACGGTa                                          <  1:1037288/76‑1 (MQ=255)

                              gCAGGTCGTCACGGTAACAAGGGCGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTa                                          >  1:1257477/1‑76 (MQ=255)

                               cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTAc                                         <  2:4073255/76‑1 (MQ=255)

                               cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTAc                                         <  2:1657555/76‑1 (MQ=255)

                                aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACg                                        >  2:2622947/1‑76 (MQ=255)

                                aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACg                                        <  2:4327113/76‑1 (MQ=255)

                                aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACg                                        >  1:675990/1‑76 (MQ=255)

                                aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACg                                        <  2:5310729/76‑1 (MQ=255)

                                  gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGcc                                      <  1:5271827/76‑1 (MQ=255)

                                   tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCg                                     >  1:6006278/1‑76 (MQ=255)

                                   tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCg                                     >  1:147093/1‑76 (MQ=255)

                                    cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCgg                                    >  1:2740023/1‑76 (MQ=255)

                                    cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCgg                                    >  1:1905325/1‑76 (MQ=255)

                                     gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGt                                   >  2:2673913/1‑76 (MQ=255)

                                     gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGt                                   <  1:2093154/76‑1 (MQ=255)

                                      tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTa                                  >  1:3612503/1‑76 (MQ=255)

                                       cACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAg                                 >  1:1133629/1‑76 (MQ=255)

                                       cACGGTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAg                                 >  1:3839968/1‑76 (MQ=255)

                                           gTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAt                             >  2:2131135/1‑76 (MQ=255)

                                           gTAACAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAt                             <  2:3724033/76‑1 (MQ=255)

                                             aaCAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCg                           >  1:3132371/1‑76 (MQ=255)

                                              aCAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGt                          <  1:1313962/76‑1 (MQ=255)

                                              aCAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGt                          <  1:4844333/76‑1 (MQ=255)

                                               cAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTa                         >  1:1379572/1‑76 (MQ=255)

                                               cAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTa                         >  2:3894865/1‑76 (MQ=255)

                                               cAAGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTa                         >  1:5166540/1‑76 (MQ=255)

                                                aaGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTAc                        >  2:633842/1‑76 (MQ=255)

                                                aaGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTAc                        >  2:5433061/1‑76 (MQ=255)

                                                aaGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTAc                        <  2:2361554/76‑1 (MQ=255)

                                                 aGGGTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACt                       >  1:2347346/1‑76 (MQ=255)

                                                 aGGGCGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACt                       >  1:4509662/1‑76 (MQ=255)

                                                  gggTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTg                      >  2:1319782/1‑76 (MQ=255)

                                                   ggTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGa                     >  2:4939334/1‑76 (MQ=255)

                                                   ggTGTAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGa                     <  1:3452904/76‑1 (MQ=255)

                                                    gtgtAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGaa                    >  1:2621545/1‑76 (MQ=255)

                                                     tgtAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAc                   >  2:1477052/1‑76 (MQ=255)

                                                      gtAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAcc                  >  2:2184991/1‑76 (MQ=255)

                                                       tAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAccc                 >  1:6007415/1‑76 (MQ=255)

                                                       tAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAccc                 >  2:4718957/1‑76 (MQ=255)

                                                       tAATTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAccc                 >  2:3531876/1‑76 (MQ=255)

                                                        aaTTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCg                >  2:191958/1‑76 (MQ=255)

                                                        aaTTTCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCg                >  1:369296/1‑76 (MQ=255)

                                                          tttCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCt              >  1:51930/1‑76 (MQ=255)

                                                          tttCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCt              >  1:1546129/1‑76 (MQ=255)

                                                           ttCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTg             >  1:2447933/1‑76 (MQ=255)

                                                           ttCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTg             >  2:4294486/1‑76 (MQ=255)

                                                           ttCTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTg             >  1:5650923/1‑76 (MQ=255)

                                                             cTAAGATCAACCTGATCGAAGATATGCCTTACGGTGAAAACGGTACGGCGGTAGACATCGTTCTGAACCCCCTggg           >  2:2043792/1‑76 (MQ=255)

                                                             cTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTggg           >  2:4147872/1‑76 (MQ=255)

                                                             cTAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTggg           >  1:1810370/1‑76 (MQ=255)

                                                              tAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGc          >  1:3057134/1‑76 (MQ=255)

                                                              tAAGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGc          >  1:354457/1‑76 (MQ=255)

                                                               aaGATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCg         <  2:1194007/76‑1 (MQ=255)

                                                                 gATCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTa       <  1:1990853/76‑1 (MQ=255)

                                                                  aTCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAc      <  2:4180277/76‑1 (MQ=255)

                                                                  aTCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAc      >  2:3427927/1‑76 (MQ=255)

                                                                  aTCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAc      >  2:1116972/1‑76 (MQ=255)

                                                                   tCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAcc     >  2:3682965/1‑76 (MQ=255)

                                                                   tCAACCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAcc     >  1:1444235/1‑76 (MQ=255)

                                                                     aaCCTGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGt   >  2:6095639/1‑76 (MQ=255)

AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTC  >  REL606/4164024‑4164169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`