| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,164,802 | A→G | E1316G (GAG→GGG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,164,802 | 0 | A | G | 99.1% | 880.7 / ‑2.4 | 215 | E1316G (GAG→GGG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (106/106); total (108/107) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
Only 100 of 219 total aligned reads displayed.
CCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGA > REL606/4164730‑4164874 | ccGTTAAGTCTGATGACGTGAACGGTCTTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGcc > 1:1394155/1‑76 (MQ=255) cGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCg > 1:5464149/1‑76 (MQ=255) cGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCg > 1:5290524/1‑76 (MQ=255) gTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCgg > 2:1041503/1‑76 (MQ=255) gTTAAGTCTGATGACGTAAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCgg < 1:4891549/76‑1 (MQ=255) ttAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCggg < 1:3369042/76‑1 (MQ=255) ttAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCggg > 2:5232875/1‑76 (MQ=255) tAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGc > 1:5809470/1‑76 (MQ=255) tAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGc > 1:2203333/1‑76 (MQ=255) aaGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCa > 2:4060931/1‑76 (MQ=255) aGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCAt < 1:4939847/76‑1 (MQ=255) gTCTGATGACGCGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATg < 1:4820904/76‑1 (MQ=255) tCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGc > 1:313855/1‑76 (MQ=255) cTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGcc > 2:1379770/1‑76 (MQ=255) cTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGcc < 1:1599927/76‑1 (MQ=255) tgatgaGGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGTGCCGGGCATGCCa < 2:1759205/76‑1 (MQ=255) tgatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCa < 1:660699/76‑1 (MQ=255) gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAg < 2:4683717/76‑1 (MQ=255) gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAg < 2:4787374/76‑1 (MQ=255) atgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGa > 2:5544898/1‑76 (MQ=255) atgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGa < 1:5687155/76‑1 (MQ=255) taCGGTCGTACCAAGATGTATAAAAACATCGTGGAGGGCAACCATCAGATGGGGCCGGGCATGCCTGTATCCTTCa > 2:3138649/2‑76 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCa > 1:964968/1‑76 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCa < 1:3670061/76‑1 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCa > 2:2107426/1‑76 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCa > 2:877078/1‑76 (MQ=255) aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCaa < 1:3926097/76‑1 (MQ=255) aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCaa < 2:1289792/76‑1 (MQ=255) cGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAAc < 2:6047014/76‑1 (MQ=255) cGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAAc < 2:362679/76‑1 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAt > 1:4216628/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAt > 1:4187003/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAt > 2:4369057/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAt > 1:106791/1‑76 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAtt < 1:3249/76‑1 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAtt > 1:5444057/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttg > 2:2488500/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttg > 1:5429321/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttg > 1:3090986/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttg < 2:1086845/76‑1 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAAGCGTCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttgt < 1:2395519/76‑1 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttgt > 2:5119435/1‑76 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttgtt < 2:4246680/76‑1 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGAATGCCAGAATCCTTCAACGTAttgtt < 1:2587925/76‑1 (MQ=255) cAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttgttg > 1:84097/1‑76 (MQ=255) cAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTAttgttg < 1:2838992/76‑1 (MQ=255) aaGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGa < 1:5755749/76‑1 (MQ=255) aGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGaa < 1:2084162/76‑1 (MQ=255) aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAag < 1:1836754/76‑1 (MQ=255) tGTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAaga < 2:2113864/76‑1 (MQ=255) gTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAagag < 2:1506341/76‑1 (MQ=255) gTATAAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAagag > 2:2559739/1‑76 (MQ=255) tataAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAgaga < 1:3794158/76‑1 (MQ=255) tataAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAgaga > 1:2168891/1‑76 (MQ=255) taAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt < 1:3820398/76‑1 (MQ=255) taAAAACATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt > 2:2036937/1‑76 (MQ=255) aaaCATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgt > 2:4553256/1‑76 (MQ=255) aaaCATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgt < 1:6214822/76‑1 (MQ=255) aCATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgttc < 2:4514499/76‑1 (MQ=255) aCATCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgttc < 1:5959361/76‑1 (MQ=255) aTCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGTGATTCGTTCGc > 1:2003136/1‑76 (MQ=255) aTCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGc > 2:5558768/1‑76 (MQ=255) aTCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGc < 1:4494928/76‑1 (MQ=255) tCGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCt < 2:5403705/76‑1 (MQ=255) cGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTg < 2:4937126/76‑1 (MQ=255) cGTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTg > 2:4608188/1‑76 (MQ=255) gTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTgg > 2:4488084/1‑76 (MQ=255) gTGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTgg < 1:3526749/76‑1 (MQ=255) tGGACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg > 1:4631620/1‑76 (MQ=255) ggACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt < 1:466587/76‑1 (MQ=255) ggACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt > 1:872326/1‑76 (MQ=255) gACGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTa > 2:483437/1‑76 (MQ=255) aCGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt < 2:3209561/76‑1 (MQ=255) aCGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt > 2:3827455/1‑76 (MQ=255) aCGGCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt > 2:5840326/1‑76 (MQ=255) cGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATc > 2:2773146/1‑76 (MQ=255) ggCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa > 1:1183402/1‑76 (MQ=255) ggCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa < 2:3984797/76‑1 (MQ=255) ggCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa < 2:5464149/76‑1 (MQ=255) ggCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa > 2:2671483/1‑76 (MQ=255) gCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCaa > 1:3337103/1‑76 (MQ=255) gCAACCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCaa < 1:4146582/76‑1 (MQ=255) aaCCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa < 1:1878091/76‑1 (MQ=255) aaCCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa < 2:3236178/76‑1 (MQ=255) aaCCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa < 2:536839/76‑1 (MQ=255) aCCATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACAt > 1:165689/1‑76 (MQ=255) cATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg < 1:4588217/76‑1 (MQ=255) cATCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg < 1:1481790/76‑1 (MQ=255) aTCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa > 1:2384811/1‑76 (MQ=255) aTCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa > 1:5227675/1‑76 (MQ=255) aTCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa > 2:774026/1‑76 (MQ=255) tCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa > 1:5670912/1‑76 (MQ=255) tCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa < 2:2279107/76‑1 (MQ=255) tCAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa > 1:5370861/1‑76 (MQ=255) cAGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAAc > 1:3979592/1‑76 (MQ=255) aGATGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACt > 2:2887235/1‑76 (MQ=255) aGATGGGGACGGGCATGCCACCATACTTCAACGTATTGTAGACAGACATTCGTTCGCTGGGTATCAACATCGAACt < 2:348324/76‑1 (MQ=255) aTGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTgg > 2:4035774/1‑76 (MQ=255) aTGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTgg > 2:6224313/1‑76 (MQ=255) tGGGGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAATATATTCGTTCGCTGGGTATCAACATCGAACTGGa < 2:1312937/76‑1 (MQ=255) | CCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGA > REL606/4164730‑4164874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |