| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,302,867 | A→T | D100E (GAT→GAA) | phnD ← | phosphonate/organophosphate ester transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,302,867 | 0 | A | T | 99.2% | 487.3 / NA | 134 | D100E (GAT→GAA) | phnD | phosphonate/organophosphate ester transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (90/43); minor base G (1/0); total (91/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 152 total aligned reads displayed.
CGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGATCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATA > REL606/4302794‑4302939 | cGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTc > 1:1867631/1‑76 (MQ=255) gATCACGACGCCCCAGTAACCTGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTcc < 1:2545545/76‑1 (MQ=255) gATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTcc > 2:1605755/1‑76 (MQ=255) aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 2:4543644/1‑76 (MQ=255) aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 1:3899628/1‑76 (MQ=255) aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 2:2407588/1‑76 (MQ=255) aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGACGTTGGAGCGTTCCa > 2:5556791/1‑76 (MQ=255) tCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCAc > 2:2415240/1‑76 (MQ=255) aaCACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGGTCCACCg > 2:1440113/1‑76 (MQ=255) gcGCTCCAGTAACCGGCCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt < 2:2624865/75‑1 (MQ=255) ccGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt < 1:4708853/75‑1 (MQ=255) acGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt > 2:775797/1‑76 (MQ=255) cGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTc > 1:4420845/1‑76 (MQ=255) cGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTc > 1:6010368/1‑76 (MQ=255) gCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTcc > 1:4956027/1‑76 (MQ=255) tCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAt > 1:3005802/1‑76 (MQ=255) cccAGTACCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAt < 2:605039/75‑1 (MQ=255) ccAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAtt > 1:3481665/1‑76 (MQ=255) ccAGTAACCGGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAtt < 1:5703253/76‑1 (MQ=255) cAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTg > 2:3491058/1‑76 (MQ=255) aGTAGCCGGGCGATCCACCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGc < 1:4191235/76‑1 (MQ=255) aGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTACACCGCTTCCCTTGc > 1:2694035/1‑76 (MQ=255) gTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGcc > 2:4230963/1‑76 (MQ=255) gTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGcc > 1:4298945/1‑76 (MQ=255) tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 1:4965384/1‑76 (MQ=255) tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 2:274956/1‑76 (MQ=255) tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 1:1078327/1‑76 (MQ=255) tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCAACGCTTCCATTGCCg > 1:4055840/1‑76 (MQ=255) aaCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGa > 2:2749016/1‑76 (MQ=255) aaCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGa > 2:3549315/1‑76 (MQ=255) ccgggCGATCCATCCGCCGCGACCGTCTGGGCGAAGGCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACa < 1:2688872/73‑1 (MQ=255) ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGGCa > 2:3363938/1‑76 (MQ=255) ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACa > 1:5593142/1‑76 (MQ=255) ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGACGACa > 1:3794449/1‑76 (MQ=255) cTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAg > 1:4344784/1‑76 (MQ=255) cTGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAg < 2:2659754/76‑1 (MQ=255) ggCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGt > 2:235390/1‑76 (MQ=255) gATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGc > 2:1716845/1‑76 (MQ=255) tgccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGcc < 1:4903369/74‑1 (MQ=255) cccacccGCGGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCg < 2:1078327/75‑1 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCgg > 1:2147186/1‑75 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 1:3009575/1‑76 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 2:3410820/1‑76 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 1:3702046/1‑76 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 2:3841738/1‑76 (MQ=255) ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGGTGCCGt > 1:466497/1‑76 (MQ=255) atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:2074634/1‑76 (MQ=255) atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:6181431/1‑76 (MQ=255) atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:5635243/1‑76 (MQ=255) atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:715585/1‑76 (MQ=255) atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 2:112766/1‑76 (MQ=255) tccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAcc < 1:5466463/76‑1 (MQ=255) ccgccgCGCCCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:5905585/76‑1 (MQ=255) ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa > 1:1454500/1‑76 (MQ=255) ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 2:4764947/76‑1 (MQ=255) ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:4923473/76‑1 (MQ=255) ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:6246374/76‑1 (MQ=255) cgccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAg > 1:3300960/1‑76 (MQ=255) gccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAgg < 2:656879/76‑1 (MQ=255) ccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCCGGc > 2:1475442/1‑76 (MQ=255) ccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGc > 1:5565847/1‑76 (MQ=255) cgcgCCCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCg < 2:2313042/76‑1 (MQ=255) gcccGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGata < 2:6267463/74‑1 (MQ=255) gACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGTGatt > 1:4094301/1‑75 (MQ=255) gACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGata > 1:2355561/1‑76 (MQ=255) cGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATcc > 1:5667388/1‑76 (MQ=255) gTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCa > 1:2877261/1‑76 (MQ=255) gTCGGGGCGAAGCCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCa < 1:775797/76‑1 (MQ=255) tcggggCGAAGCCCTGGCCGTTGGCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCAc < 2:5593142/73‑1 (MQ=255) cTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACt > 2:2105489/1‑76 (MQ=255) cTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACt > 2:6278353/1‑76 (MQ=255) tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt > 2:4798978/1‑76 (MQ=255) tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt < 2:2196153/76‑1 (MQ=255) tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt > 1:2637697/1‑76 (MQ=255) gggCGAAGCCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACttt < 1:5405311/76‑1 (MQ=255) ggCAAAGACCTGGCGGTTGGCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTa < 1:955150/76‑1 (MQ=255) gCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTAt > 1:2931772/1‑76 (MQ=255) gAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTg > 2:3813066/1‑76 (MQ=255) gAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTg > 1:3398029/1‑76 (MQ=255) aaGACCTGGCCGTTGGCGCGTTCCGCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:3353103/1‑76 (MQ=255) aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:1159734/1‑76 (MQ=255) aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:5232621/1‑76 (MQ=255) aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:135073/1‑76 (MQ=255) aggccTGGCCGTTGGCGCGTTCCACCGCTCCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGaa < 2:2944234/73‑1 (MQ=255) gcccTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAg < 2:1852943/74‑1 (MQ=255) aCCTGGTCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgc > 1:3479382/1‑76 (MQ=255) aCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgc < 1:2304473/76‑1 (MQ=255) cTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc > 1:1678477/1‑76 (MQ=255) tGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCa > 2:1664180/1‑76 (MQ=255) ggCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAt > 1:848797/1‑76 (MQ=255) gCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAtt < 2:658318/76‑1 (MQ=255) ccGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTc < 2:3556637/76‑1 (MQ=255) cGTTGGCGCTTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTcc < 1:5399280/76‑1 (MQ=255) gtgggCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTccc < 2:3719610/73‑1 (MQ=255) ttGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTCCCGGGCGATATCCACTTTATTGAAGCGCATTCCCt < 2:5461155/76‑1 (MQ=255) tgggCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCt < 2:1885839/74‑1 (MQ=255) tGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTg > 1:1514467/1‑76 (MQ=255) gggCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTg < 2:3389433/75‑1 (MQ=255) gcgTTCCGCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATa < 1:1099261/76‑1 (MQ=255) gcgTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATa < 2:5974834/76‑1 (MQ=255) | CGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGATCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATA > REL606/4302794‑4302939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |