| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,523,210 | C→T | T87I (ACC→ATC) | fimE → | tyrosine recombinase/inversion of on/off regulator of fimA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,523,210 | 0 | C | T | 99.4% | 690.7 / ‑4.2 | 174 | T87I (ACC→ATC) | fimE | tyrosine recombinase/inversion of on/off regulator of fimA |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (75/98); total (76/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01 | |||||||||||
Only 100 of 182 total aligned reads displayed.
TCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGC > REL606/4523136‑4523281 | cGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGAtcc > 2:178923/1‑76 (MQ=255) ccGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAg < 2:1485179/76‑1 (MQ=255) cGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAgg < 1:1101054/76‑1 (MQ=255) cGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAgg > 1:932383/1‑76 (MQ=255) gACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGa < 1:4069202/76‑1 (MQ=255) gACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGa < 1:4825301/76‑1 (MQ=255) aCTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGaa > 2:3983309/1‑76 (MQ=255) cTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAAc > 1:623662/1‑76 (MQ=255) tGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACg > 1:2708494/1‑76 (MQ=255) gaagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGt > 1:1562721/1‑76 (MQ=255) gaagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGt < 2:170495/76‑1 (MQ=255) gaagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGt < 1:2074508/76‑1 (MQ=255) gaagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGt < 1:4929628/76‑1 (MQ=255) gaagaaCGGATTTTCAACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGt < 1:1672444/76‑1 (MQ=255) aagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTg < 2:771141/76‑1 (MQ=255) aagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTg > 2:2923987/1‑76 (MQ=255) agaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGCTGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGc > 1:4513174/1‑76 (MQ=255) aaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTa < 2:1065207/76‑1 (MQ=255) aaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTa > 1:4683917/1‑76 (MQ=255) ggATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACt < 2:2181234/76‑1 (MQ=255) gATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTg < 2:1397518/76‑1 (MQ=255) gATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTg > 1:6113413/1‑76 (MQ=255) aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTgg > 2:3038680/1‑76 (MQ=255) aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTgg > 2:2610069/1‑76 (MQ=255) aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTgg < 2:5658246/76‑1 (MQ=255) aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTgg < 1:1375589/76‑1 (MQ=255) aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTgg < 1:1937785/76‑1 (MQ=255) ttttCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGa < 2:4946644/76‑1 (MQ=255) tttCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGaa < 1:1533625/76‑1 (MQ=255) tttCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGaa < 1:2327175/76‑1 (MQ=255) ttCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGaaa < 2:29582/76‑1 (MQ=255) ttCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGaaa < 2:5001568/76‑1 (MQ=255) cTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAgg > 1:1430646/1‑76 (MQ=255) cTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAgg > 1:3314600/1‑76 (MQ=255) cTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAgg > 1:774376/1‑76 (MQ=255) cGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCt < 1:4422971/76‑1 (MQ=255) cGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCt > 2:4745821/1‑76 (MQ=255) cGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCt < 1:3148309/76‑1 (MQ=255) cGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCt < 1:5733054/76‑1 (MQ=255) gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTg < 1:3975190/76‑1 (MQ=255) gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTg > 2:198772/1‑76 (MQ=255) gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTg < 2:589232/76‑1 (MQ=255) gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTg < 2:847676/76‑1 (MQ=255) ttCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGa > 1:1932645/1‑76 (MQ=255) ttCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGa < 2:1227210/76‑1 (MQ=255) tCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGAc > 2:2293809/1‑76 (MQ=255) tCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGAc > 2:5270872/1‑76 (MQ=255) cACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGAcc > 1:1203745/1‑76 (MQ=255) aCCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCg < 2:3398080/76‑1 (MQ=255) aCCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCg < 1:5857829/76‑1 (MQ=255) cccGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCgg < 1:1786600/76‑1 (MQ=255) ccGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCggc > 2:4698678/1‑75 (MQ=255) ccGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGa > 2:236978/1‑76 (MQ=255) cGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGAc < 2:2169598/76‑1 (MQ=255) cGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACATGCTAACTGGAAAGGCGCTGACCGGAc > 2:1512834/1‑76 (MQ=255) gTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACt < 2:652292/76‑1 (MQ=255) ttACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTg < 2:3213584/76‑1 (MQ=255) tACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGa > 2:119542/1‑76 (MQ=255) tACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGa < 2:1908973/76‑1 (MQ=255) tACGTGTTGAGGAGCGTGAAGCCGTGTAGCGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCAGACTGa < 2:2464168/76‑1 (MQ=255) cGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACg < 1:2616303/76‑1 (MQ=255) gTTTTGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGc > 2:6033731/1‑76 (MQ=255) ttttGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCt > 1:4325755/1‑76 (MQ=255) tggtgacgaGCGGGAAGCCGTGGAGCGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCt < 2:983303/73‑1 (MQ=255) tttGATGATCTTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCta < 2:2692166/76‑1 (MQ=255) tttGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCta < 2:5161245/76‑1 (MQ=255) tttGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCta < 1:3232971/76‑1 (MQ=255) tttGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCta > 1:5283388/1‑76 (MQ=255) ttGATGAGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtat > 2:868255/1‑76 (MQ=255) tgatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtata > 1:1289915/1‑76 (MQ=255) tgatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtata < 1:852771/76‑1 (MQ=255) tgatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGCGCTAACTGGAAAGGCGCTGACCGGACTGACGCtata < 2:5754285/76‑1 (MQ=255) gatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTatat < 2:2663659/76‑1 (MQ=255) gatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTatat > 2:1314492/1‑76 (MQ=255) gatgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTatat < 1:5267667/76‑1 (MQ=255) atgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAACGGCGCTGACCGGACTGACGTGAtatt > 2:3656548/1‑76 (MQ=255) atgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTAtatt < 2:679969/76‑1 (MQ=255) atgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTAtatt > 2:238983/1‑76 (MQ=255) tgaGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTAtattt > 2:2838315/1‑76 (MQ=255) aGCGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTAtatttat > 1:3521247/1‑76 (MQ=255) cGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATatttattt > 2:42927/1‑76 (MQ=255) cGTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATatttattt < 1:6125754/76‑1 (MQ=255) gTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtc > 1:4882150/1‑76 (MQ=255) gTGAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtc > 2:6278655/1‑76 (MQ=255) gAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtctc < 2:4380911/76‑1 (MQ=255) gAAGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtctc < 1:5986091/76‑1 (MQ=255) aaGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTcg < 2:6247333/76‑1 (MQ=255) aaGCCGTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTcg < 2:5443040/76‑1 (MQ=255) gTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCcgcg > 1:1059976/1‑76 (MQ=255) gTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCcgcg > 2:3559159/1‑76 (MQ=255) gTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCcgcg < 1:3663440/76‑1 (MQ=255) gTGGAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTAGCcgcg > 2:1655495/1‑76 (MQ=255) gAACGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGa < 1:104892/76‑1 (MQ=255) aaCGCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAg > 2:4856233/1‑76 (MQ=255) gCTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCg > 2:4133773/1‑76 (MQ=255) cTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCgg > 1:684349/1‑76 (MQ=255) cTGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCgg > 1:3579770/1‑76 (MQ=255) tGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGc < 2:1371970/76‑1 (MQ=255) tGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGc > 1:5392646/1‑76 (MQ=255) tGGATCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGc > 1:2213882/1‑76 (MQ=255) | TCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGC > REL606/4523136‑4523281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |