BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,608,514	T→C	V351A (GTC→GCC)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,608,514	0	T	C	99.3%	613.6 / ‑5.2	152	V351A (GTC→GCC)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (79/72); total (80/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 157 total aligned reads displayed.

GTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCATCTGA  >  REL606/4608441‑4608586

gTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCa                                                                         <  2:2691652/76‑1 (MQ=255)

gTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCa                                                                         >  1:5407051/1‑76 (MQ=255)

gTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCa                                                                         <  1:5304710/76‑1 (MQ=255)

  tGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGt                                                                       <  2:3912317/76‑1 (MQ=255)

  tGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGt                                                                       >  2:2046321/1‑76 (MQ=255)

  tGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGt                                                                       <  1:307154/76‑1 (MQ=255)

   gagaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTg                                                                      >  2:3771405/1‑76 (MQ=255)

   gagaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTg                                                                      <  1:32331/76‑1 (MQ=255)

    agaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGa                                                                     >  2:5030573/1‑76 (MQ=255)

    agaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGa                                                                     >  2:1457233/1‑76 (MQ=255)

     gaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGaa                                                                    <  1:1071129/76‑1 (MQ=255)

     gaACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGaa                                                                    <  1:1563552/76‑1 (MQ=255)

      aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGaaa                                                                   <  1:1038139/76‑1 (MQ=255)

      aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGaaa                                                                   <  2:3794302/76‑1 (MQ=255)

      aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTGTGCCCGTTaaa                                                                   >  2:754751/1‑76 (MQ=255)

       aCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAAt                                                                  <  1:6065820/76‑1 (MQ=255)

        cTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGCAATg                                                                 >  2:1361203/1‑76 (MQ=255)

        cTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATg                                                                 >  2:5624948/1‑76 (MQ=255)

           cgcgAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGAt                                                              <  1:6302549/76‑1 (MQ=255)

            gcgAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATa                                                             <  1:3280223/76‑1 (MQ=255)

             cgAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAc                                                            <  2:2125097/76‑1 (MQ=255)

             cgAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAc                                                            >  1:5443245/1‑76 (MQ=255)

              gAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAcc                                                           <  1:2907979/76‑1 (MQ=255)

              gAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAcc                                                           <  1:818916/76‑1 (MQ=255)

              gAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAcc                                                           >  2:2674854/1‑76 (MQ=255)

               aaGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAccc                                                          >  2:3194176/1‑76 (MQ=255)

               aaGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAacc                                                          >  2:4214797/1‑76 (MQ=255)

                aGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCcg                                                         <  1:5177632/76‑1 (MQ=255)

                  tATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCcgcg                                                       <  1:4181755/76‑1 (MQ=255)

                  tATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACcgcg                                                       >  2:3143974/1‑76 (MQ=255)

                  tATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACcgcg                                                       >  2:5028437/1‑76 (MQ=255)

                  tATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACcccg                                                       >  1:208448/1‑76 (MQ=255)

                    tCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCcgcgcg                                                     <  2:4441325/76‑1 (MQ=255)

                    tCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCcgcgcg                                                     <  2:5958970/76‑1 (MQ=255)

                     cTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCccgcgc                                                    >  2:5828126/1‑76 (MQ=255)

                       gCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCCCGCGCTg                                                  >  1:3953958/1‑76 (MQ=255)

                         cGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTggg                                                >  1:1617/1‑76 (MQ=255)

                         cGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTggg                                                <  1:3926567/76‑1 (MQ=255)

                          gACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTgggg                                               >  1:3503489/1‑76 (MQ=255)

                          gACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTgggg                                               >  2:1141267/1‑76 (MQ=255)

                          gACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTgggg                                               >  1:5184665/1‑76 (MQ=255)

                           aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGa                                              <  2:5251220/76‑1 (MQ=255)

                           aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGa                                              <  2:255177/76‑1 (MQ=255)

                           aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGa                                              <  2:4744453/76‑1 (MQ=255)

                            cAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGAt                                             <  1:2809413/76‑1 (MQ=255)

                            cAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGAt                                             <  2:6127246/76‑1 (MQ=255)

                            cAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTggggga                                             >  2:2731107/1‑74 (MQ=255)

                             aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATg                                            <  1:1998989/76‑1 (MQ=255)

                               cGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGAgggg                                          >  2:5236961/1‑75 (MQ=255)

                               cGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATggt                                          <  1:5328987/76‑2 (MQ=255)

                               cGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTGGGGATggg                                          >  2:4093329/1‑75 (MQ=255)

                                gATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCa                                         >  2:1133366/1‑76 (MQ=255)

                                gATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCa                                         >  1:4957412/1‑76 (MQ=255)

                                 aTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAg                                        <  1:558310/76‑1 (MQ=255)

                                     tGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGAGGGCTGCGGt                                    >  2:686149/1‑76 (MQ=255)

                                      gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAgggggt                                   >  2:1427321/1‑74 (MQ=255)

                                      gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGtt                                   <  1:2715096/76‑1 (MQ=255)

                                      gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTTGGGATGGGAGTgggt                                   >  2:1977874/1‑74 (MQ=255)

                                       aCGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg                                  <  2:6011137/76‑1 (MQ=255)

                                       aCGAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg                                  <  2:5418353/76‑1 (MQ=255)

                                         gAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑Ca                               <  1:3782233/76‑1 (MQ=255)

                                         gAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑Ca                               <  1:3974152/76‑1 (MQ=255)

                                         gAAAGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑Ca                               >  1:1369563/1‑76 (MQ=255)

                                          aaaGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCCCGCGCTGGGGATGGTAGCTGTTT‑Caa                              >  2:1872885/1‑76 (MQ=255)

                                           aaGCAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑GAAt                             >  1:2769538/1‑76 (MQ=255)

                                              cAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATggg                          >  1:4024116/1‑76 (MQ=255)

                                              cAGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTGGGGATGGCAGTGGGTG‑GAATtgg                          >  2:3372166/1‑76 (MQ=255)

                                               aGTCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCCCGCGCTGGGGATGGCAGTGGTTG‑GAATgggg                         >  2:3873543/1‑75 (MQ=255)

                                                 tCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTG‑CAATgggggg                       >  2:4347150/1‑76 (MQ=255)

                                                 tCTATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGggcgg                       >  1:2803074/1‑76 (MQ=255)

                                                   tATGCTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑GAATgggggggg                     >  2:5553178/1‑74 (MQ=255)

                                                       cTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCCAATGgggggggggg                  >  2:5443090/1‑74 (MQ=255)

                                                       cTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGGGGGGggcg                 >  2:5192841/1‑76 (MQ=255)

                                                       cTGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGGTG‑CAATGGGGggcgggcg                 >  1:3865962/1‑76 (MQ=255)

                                                       cTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTGGGGATGGCAGTGGTTG‑GCATGggggggggggc                 >  1:2384212/1‑73 (MQ=255)

                                                       cTGATACCGAAGGTTTTGCCAGTGAAATGGATAACCGCGCGCTGGGGATGGCAGTGGTTG‑CAATTGGGGGGGggcg                 >  2:1091224/1‑76 (MQ=255)

                                                        tGATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGgtcgt                <  2:2971312/76‑1 (MQ=255)

                                                         gATACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGggcgtc               >  1:4717729/1‑76 (MQ=255)

                                                          aTACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGggcggcgggcggcg              >  1:2336866/1‑76 (MQ=255)

                                                          aTACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGgtcgtcg              <  2:4797738/76‑1 (MQ=255)

                                                          aTACCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGAGGGCAGTGGTTG‑GAATGGGGGGCGggggtcg              >  2:2836778/1‑76 (MQ=255)

                                                            aCCGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGgtcgtcgtc            <  2:5960662/76‑1 (MQ=255)

                                                             ccGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGGTG‑CAATGGGCGGGGggcgtcggcc           >  2:183517/1‑73 (MQ=255)

                                                             ccGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGGGGTTG‑CAATGGGCGGCGGGCGTCGTCa           >  1:910674/1‑76 (MQ=255)

                                                              cGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAg          <  2:4735388/76‑1 (MQ=255)

                                                              cGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAg          <  1:6287136/76‑1 (MQ=255)

                                                              cGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGGCGTCGTCAg          >  1:720057/1‑76 (MQ=255)

                                                              cGAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGGGGTTG‑CCATGGGCGGGGggcgtcgtcca          >  2:3682340/1‑74 (MQ=255)

                                                               gAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGTGCGGCGGTCGTCGTCAgg         <  2:4807949/76‑1 (MQ=255)

                                                               gAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAgg         <  1:572375/76‑1 (MQ=255)

                                                               gAAGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGGGGTTG‑CAATGGGCGGCGGCGGGCGTCAgg         >  1:1387109/1‑76 (MQ=255)

                                                                aaGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGGCGTCGCCAGGc        >  2:4534626/1‑76 (MQ=255)

                                                                 aGGTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCa       <  2:6307449/76‑1 (MQ=255)

                                                                  ggTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCAt      <  1:996582/76‑1 (MQ=255)

                                                                  ggTTTTGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCAt      <  1:6158602/76‑1 (MQ=255)

                                                                    ttttGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCATCt    <  2:2300022/76‑1 (MQ=255)

                                                                    ttttGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGCCGGCCGGCctct    >  1:6147638/1‑76 (MQ=255)

                                                                    ttttGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGGCGTCGTCAGGCATCt    >  1:4743674/1‑76 (MQ=255)

                                                                      ttGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCATCTGa  <  2:3326126/76‑1 (MQ=255)

                                                                      ttGCCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGGTG‑CAATGGGCGGCGGGCGTCCGCAGGCCTCTGa  >  1:1755801/1‑76 (MQ=255)

GTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG‑CAATGGGCGGCGGTCGTCGTCAGGCATCTGA  >  REL606/4608441‑4608586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`