| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,602,152 | 2 bp→AT | intergenic (+175/+52) | pinQ → / ← ECB_01505 | predicted site‑specific recombinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,602,152 | 0 | T | A | 98.8% | 331.6 / ‑3.5 | 83 | intergenic (+175/+53) | pinQ/ECB_01505 | predicted site‑specific recombinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (35/47); total (35/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| * | REL606 | 1,602,153 | 0 | G | T | 98.8% | 327.0 / ‑3.7 | 83 | intergenic (+176/+52) | pinQ/ECB_01505 | predicted site‑specific recombinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (35/47); total (35/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TGCAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCTGGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCAA > REL606/1602105‑1602222 || tgCAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTtcctcc < 2:6196064/76‑1 (MQ=255)tgCAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTtcctcc < 2:770563/76‑1 (MQ=255)tgCAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTtcctcc < 2:770564/76‑1 (MQ=255) cAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGt > 1:1063823/1‑76 (MQ=255) aGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGtt < 2:2315904/76‑1 (MQ=255) aGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGtt > 1:4763606/1‑76 (MQ=255) ttGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGc < 2:6300231/76‑1 (MQ=255) tGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCa > 2:3411361/1‑76 (MQ=255) tGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCa < 1:282968/76‑1 (MQ=255) aCGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCata < 2:5817806/76‑1 (MQ=255) cGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCatat < 2:2375910/76‑1 (MQ=255) cGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCatat < 1:2240931/76‑1 (MQ=255) cGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCatat > 1:2522390/1‑76 (MQ=255) cGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCatat < 1:5892438/76‑1 (MQ=255) tATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATAc < 1:1601603/76‑1 (MQ=255) tATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATAc > 2:1838676/1‑76 (MQ=255) tATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATAc < 1:2728526/76‑1 (MQ=255) tATCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATAc > 2:255326/1‑76 (MQ=255) aTCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACa < 1:518610/76‑1 (MQ=255) aTCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACa > 2:4074005/1‑76 (MQ=255) aTCAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACa < 1:4298858/76‑1 (MQ=255) cAACACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGc > 1:2390782/1‑76 (MQ=255) aaCACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCt < 1:2599392/76‑1 (MQ=255) aaCACGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCt > 1:1567369/1‑76 (MQ=255) acacGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTc < 1:3370861/76‑1 (MQ=255) acacGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTc < 2:3882051/76‑1 (MQ=255) cacGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCa < 2:4404085/76‑1 (MQ=255) cacGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCa > 2:876187/1‑76 (MQ=255) cacGGTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCa < 2:4332160/76‑1 (MQ=255) gTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtt > 2:3326213/1‑76 (MQ=255) gTTCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtt < 2:3484692/76‑1 (MQ=255) ttCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCttt > 1:1732151/1‑76 (MQ=255) ttCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCttt < 2:4417051/76‑1 (MQ=255) tCAGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtttt < 2:5668884/76‑1 (MQ=255) aGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtttttt < 1:5345227/76‑1 (MQ=255) aGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtttttt > 2:5723004/1‑76 (MQ=255) aGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtttttt < 2:6044238/76‑1 (MQ=255) aGCAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCtttttt > 1:3327984/1‑76 (MQ=255) gCAACACCCGATACTTTTTCCTGGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTc < 1:2231130/76‑1 (MQ=255) cAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCa < 2:3888384/76‑1 (MQ=255) cAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCa > 2:4189275/1‑76 (MQ=255) cAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCa < 1:5629392/76‑1 (MQ=255) cAACACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCa > 2:1181244/1‑76 (MQ=255) aaCACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAc > 2:622940/1‑76 (MQ=255) aaCACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAc > 1:1139251/1‑76 (MQ=255) aaCACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAc < 1:5360790/76‑1 (MQ=255) aaCACCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAc > 1:5239519/1‑76 (MQ=255) acacCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAcc > 2:3835753/1‑76 (MQ=255) cacCCGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAccc < 1:4374304/76‑1 (MQ=255) cacCCGATACTTTTTCCATGCTTAAAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAccc > 2:4194965/1‑76 (MQ=11) cacCCGATACTTGTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCAccc < 2:667387/76‑1 (MQ=11) ccccGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCa < 2:984581/75‑1 (MQ=255) cccGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCAc < 1:5106451/76‑1 (MQ=255) ccGATACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACg < 1:3375390/76‑1 (MQ=255) aTACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGAtt < 2:532670/76‑1 (MQ=255) aTACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGAtt > 2:2960614/1‑76 (MQ=255) aTACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGAtt > 2:4356431/1‑76 (MQ=255) tACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTa < 1:5081699/76‑1 (MQ=255) tACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTa < 2:3809602/76‑1 (MQ=255) tACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTa > 2:2701736/1‑76 (MQ=255) tACTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTa < 1:6214923/76‑1 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa < 2:1893008/76‑1 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa < 1:241400/76‑1 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa > 1:1545774/1‑76 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa < 1:729286/76‑1 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa > 2:924768/1‑76 (MQ=255) aCTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTaa < 2:1992867/76‑1 (MQ=255) cTTTTTCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAc > 2:249290/1‑76 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc > 2:2981666/1‑76 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc < 2:451087/76‑1 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc < 1:5267713/76‑1 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc < 1:3475471/76‑1 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc > 1:1846850/1‑76 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc > 1:1717509/1‑76 (MQ=255) tttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAAcc > 1:1606863/1‑76 (MQ=255) ttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCa > 1:5806226/1‑76 (MQ=255) ttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCa < 2:4086093/76‑1 (MQ=255) ttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCa < 1:5109913/76‑1 (MQ=255) ttttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCa > 1:2581430/1‑76 (MQ=255) tttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCaa > 1:3955730/1‑76 (MQ=255) tttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCaa > 1:3356258/1‑76 (MQ=255) tttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCaa < 1:4003975/76‑1 (MQ=255) tttCCATGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCaa < 2:3878946/76‑1 (MQ=255) || TGCAGTTGACGTATCAACACGGTTCAGCAACACCCGATACTTTTTCCTGGCTTCCAGCAACGGGGTTTCTTCCTCCGTTGCATATACAGCTCACCTTTTTTCACCCACGATTAACCAA > REL606/1602105‑1602222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |