Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,212,474	Δ1 bp	intergenic (‑310/‑215)	lysC ← / → pgi	aspartate kinase III/glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,212,472	0	T	.	100.0%	309.1 / NA	62	intergenic (‑308/‑217)	lysC/pgi	aspartate kinase III/glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (39/23);  total (39/23)

TCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCG  >  REL606/4212397‑4212542                                                                            |                                                                       tCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAt                                                                        >  2:1808254/1‑76 (MQ=255) tCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAt                                                                        <  1:1630461/76‑1 (MQ=255) tCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAt                                                                        <  1:1439192/76‑1 (MQ=255) tCACAGGACTGGCGTCTCCCACACCGTTACTTGGGCAACGGTCGTCTTCTGGCCAGCGCTAGCGCAGATAGTACAt                                                                        <  2:41409/76‑1 (MQ=255)  cacaGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAtt                                                                       <  1:836946/76‑1 (MQ=255)   acaGGACTGGCTCCTCCAACACGGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAtta                                                                      <  2:1377013/76‑2 (MQ=255)   acaGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAtta                                                                      <  2:1393528/76‑2 (MQ=255)    caGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCTCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAttat                                                                     <  1:1712387/76‑3 (MQ=255)    caGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCCGGTAGTACAttat                                                                     >  2:959431/1‑74 (MQ=255)    caGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAttat                                                                     >  2:1851949/1‑74 (MQ=255)     aGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAttata                                                                    <  2:917113/76‑4 (MQ=255)     aGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACAttata                                                                    >  1:486115/1‑73 (MQ=255)       gACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTataaa                                                                 <  1:1650580/76‑1 (MQ=255)        aCTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTataaat                                                                <  1:1065907/76‑1 (MQ=255)         cTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTataaata                                                               <  2:245536/76‑1 (MQ=255)         cTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTataaata                                                               <  1:275526/76‑1 (MQ=255)         cTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGCAGTACA‑TTataaata                                                               >  2:413068/1‑76 (MQ=255)          tGGCTCCTCCAACACCGTTAGTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGTGCAGGTAGTACA‑TTataaataa                                                              >  2:160400/1‑76 (MQ=255)          tGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTataaataa                                                              <  1:1293911/76‑1 (MQ=255)           ggCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTAtaaataaa                                                             >  2:973502/1‑76 (MQ=255)            gCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGTGCTAGCGCAGGTAGTACA‑TTATAAATAAAg                                                            <  2:619372/76‑1 (MQ=255)             ctcctcCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAgg                                                           <  1:823499/76‑1 (MQ=255)               cctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGt                                                         <  2:1141893/76‑1 (MQ=255)               cctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGt                                                         >  2:1854697/1‑76 (MQ=255)               cctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGt                                                         >  2:200648/1‑76 (MQ=255)               cctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGt                                                         >  2:382822/1‑76 (MQ=255)                ctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTg                                                        <  2:1708161/76‑1 (MQ=255)                ctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTg                                                        >  2:209008/1‑76 (MQ=255)                ctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTg                                                        >  1:880583/1‑76 (MQ=255)                ctccAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTg                                                        <  1:1087978/76‑1 (MQ=255)                    aacaacGATACTTGGGAAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCg                                                    <  1:29208/76‑1 (MQ=255)                      cacCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCggg                                                  >  2:1384983/1‑76 (MQ=255)                        ccNTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTTAGCGCNGc                                                <  2:1673090/76‑1 (MQ=255)                         cGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCg                                               >  2:643094/1‑76 (MQ=255)                          gTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCgg                                              >  1:364540/1‑76 (MQ=255)                          gTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCgg                                              <  2:739942/76‑1 (MQ=255)                            tACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGCCGGtt                                            <  1:1916954/76‑1 (MQ=255)                                tGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCa                                        >  1:602428/1‑76 (MQ=255)                                 gggCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCaa                                       <  1:1334079/76‑1 (MQ=255)                                   gCAACGCGCCTCTTNTGGCCTGCGCTAGCGCAGGTAGTACA‑TGATAAATAAAGGGTGAGCTGGTCGGTTGTCAACg                                     <  1:1747243/76‑1 (MQ=255)                                    cAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGa                                    <  2:1047035/76‑1 (MQ=255)                                     aaCGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGAt                                   >  1:1002649/1‑76 (MQ=255)                                     aaCGCGACTCNTCCGTCCTGCNCTAGCGCAGGGAGTACA‑TTATAAATAAAGAGTGAGCGGGGCGGTTGTCAACGAt                                   >  2:2000907/1‑76 (MQ=255)                                      aCGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATg                                  <  2:220898/76‑1 (MQ=255)                                       cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATNAATAAAGGGTGAGCGGGGNGGNTGTCAACGATgg                                 >  1:1667487/1‑76 (MQ=255)                                       cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGAAgg                                 >  1:377436/1‑76 (MQ=255)                                        gcgcCTCTTCTGGCCTGCGCTAGCGCAGGCAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAAAGAAggg                                >  2:990584/1‑76 (MQ=255)                                           ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTc                             <  2:375434/76‑1 (MQ=255)                                           ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTc                             >  2:1516155/1‑76 (MQ=255)                                           ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTc                             <  2:1711228/76‑1 (MQ=255)                                           ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTc                             <  2:1678442/76‑1 (MQ=255)                                            ctctTCTGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCa                            >  1:833176/1‑76 (MQ=255)                                             tcttctGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCAt                           <  2:1405199/76‑1 (MQ=255)                                              cttctGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATg                          >  1:763013/1‑76 (MQ=255)                                                tctGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCg                        >  1:455018/1‑76 (MQ=255)                                                tctGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCg                        >  2:837838/1‑76 (MQ=255)                                                 ctGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCgg                       >  2:1123420/1‑76 (MQ=255)                                                  tGGCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGa                      >  2:1055807/1‑76 (MQ=255)                                                   ngCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGNGTGAGCGGGGCGGTTGTCTACGATGGGGTCATGCGGAt                     >  1:1996544/2‑76 (MQ=255)                                                   ggCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGAt                     >  2:1040192/1‑76 (MQ=255)                                                   ggCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGAt                     >  1:1265367/1‑76 (MQ=255)                                                   ggCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGAt                     >  2:754212/1‑76 (MQ=255)                                                   ggCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGAGTCATGCGGAt                     >  1:1522442/1‑76 (MQ=255)                                                   ggCCTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTCTCAACGATGGGGTCATGCGGAt                     >  2:179896/1‑76 (MQ=255)                                                     ccTGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGAttt                   >  2:532901/1‑76 (MQ=255)                                                       tGCGCTAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGAttttt                 >  2:941272/1‑76 (MQ=255)                                                        gcgcTAGCGCAGGTAGTACA‑TTATAAATAAAGGGAGAGCGGGGCGGTTGTCAACGCTGGGGGCATGCGGATTTTTc                >  2:582609/1‑76 (MQ=255)                                                            tAGCGCAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATcc            >  1:1530157/1‑76 (MQ=255)                                                             aGCGCAGGTAGTACA‑TTACAANTANAGGGTGAGCGGGGCGGTTGTCNACGATGGNGNCATGCGGATTTTTCATCCa           >  2:301489/1‑76 (MQ=255)                                                               cgcAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACt         >  2:1421459/1‑76 (MQ=255)                                                               cgcAGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCAGATTTTTCATCCACt         >  1:1681124/1‑76 (MQ=255)                                                                  aGGTAGTACA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCt      <  1:271903/76‑1 (MQ=255)                                                                    gtagtaCA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTgg    >  2:500949/1‑76 (MQ=255)                                                                     tagtaCA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGc   >  2:928464/1‑76 (MQ=255)                                                                      agtaCA‑TTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTggtg  >  1:900309/1‑74 (MQ=255)                                                                            |                                                                       TCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCG  >  REL606/4212397‑4212542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑