| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,500,634 | T→G | K57T (AAG→ACG) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,500,634 | 0 | T | G | 97.0% | 250.9 / ‑2.8 | 66 | K57T (AAG→ACG) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (27/37); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GGGTTATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCC > REL606/3500563‑3500705 | gggTTATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTAcc > 2:979841/1‑76 (MQ=255)gggTTATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTAcc > 2:492551/1‑76 (MQ=255) gTTATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTAccgc > 1:1250111/1‑76 (MQ=255) ttATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTAccgcc < 1:992004/76‑1 (MQ=255) aTCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGa < 2:1504043/76‑1 (MQ=255) aCGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAgt < 1:1424061/76‑1 (MQ=255) cGCCCATACGACGGATCCCGGAGATGCNGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAgtg > 2:7828/1‑76 (MQ=255) gCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAgtgt < 2:895653/76‑1 (MQ=255) gCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAgtgt > 1:1602636/1‑76 (MQ=255) gCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAgtgt < 2:1535424/76‑1 (MQ=255) cccATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTa > 1:628081/1‑76 (MQ=255) ccATACGACGGATCCCGGAGTTGATGCAGTTAGTCAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTAc < 1:570838/76‑1 (MQ=255) ccATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTAc < 1:806536/76‑1 (MQ=255) ccATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTAc < 1:1016737/76‑1 (MQ=255) cATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACg < 1:653786/76‑1 (MQ=255) cATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGACGAAGTGTACg < 1:116372/76‑1 (MQ=255) cANACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGTAGTGGNCg < 2:1660364/76‑1 (MQ=255) aTACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACgg < 1:440693/76‑1 (MQ=255) aTACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACgg > 1:684860/1‑76 (MQ=255) cgacgGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCg < 2:1229362/76‑1 (MQ=255) cgacgGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCg > 2:1033067/1‑76 (MQ=255) cgacgGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCg < 2:357205/76‑1 (MQ=255) gATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGtttt > 2:1291161/1‑76 (MQ=255) gATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGtttt < 2:1498410/76‑1 (MQ=255) gATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGtttt < 1:1402552/76‑1 (MQ=255) cccGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATTATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCt < 2:1758094/76‑1 (MQ=255) cccGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCt > 1:1293747/1‑76 (MQ=255) cccGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCt < 2:684859/76‑1 (MQ=255) cGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCg < 2:929370/76‑1 (MQ=255) cGGAGTTGATGCAGCTAGATAGCGCAAAGTCGATAATGCGTAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCg < 2:44107/76‑1 (MQ=255) ggAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGc < 1:886309/76‑1 (MQ=255) gAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCt < 2:607200/76‑1 (MQ=255) aGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCtt < 2:1268540/76‑1 (MQ=255) aGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACTGCCGAAGTGTACGACCGGTTTTTTTCGCtt > 1:36020/1‑76 (MQ=255) gTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTa > 2:1724314/1‑76 (MQ=255) tGCAGTGAGACAGCGCAAAGTGGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGNTTATTTGt < 1:1960580/76‑1 (MQ=255) gCAGTTAGACGGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGtt < 1:1570051/76‑1 (MQ=255) cAGTTAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTa < 1:484303/76‑1 (MQ=255) tAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATc < 2:1177159/76‑1 (MQ=255) tAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATc < 1:1633837/76‑1 (MQ=255) tAGACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATc > 2:452633/1‑76 (MQ=255) gACAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCt < 1:190158/76‑1 (MQ=255) aCAGCGCAAAGTCGNTAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATGGGTTAAATCCtt < 1:1746000/76‑1 (MQ=255) cAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTc < 1:1492128/76‑1 (MQ=255) cAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTc < 2:812412/76‑1 (MQ=255) cAGCGCAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTc > 2:1282796/1‑76 (MQ=255) cAAAGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCg > 2:1959003/1‑76 (MQ=255) aaaGTCGATCATGCGGAGCGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTNAANTCTTCAGGCgg > 2:328146/1‑76 (MQ=255) aaaGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCgg < 1:595008/76‑1 (MQ=255) aaaGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCgg > 2:68376/1‑76 (MQ=255) aaaGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCgg > 2:1868294/1‑76 (MQ=255) aaaGTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGCCTTTGCTCGATTATTGGTTAAGTCCNTCAGGCgg > 2:348924/1‑76 (MQ=255) gTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCgggca > 2:461711/1‑74 (MQ=255) gTCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTa < 2:657233/76‑1 (MQ=255) gTCGATAATGAGGAACGGACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTa > 1:159635/1‑76 (MQ=255) tCGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTAc > 2:1825275/1‑76 (MQ=255) cGATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTAcc > 1:834503/1‑76 (MQ=255) gATAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCa < 1:973535/76‑1 (MQ=255) gATAATGCCGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCa > 1:1889501/1‑76 (MQ=255) aTAATGCGGAATGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCAc > 1:51065/1‑76 (MQ=255) aTAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTACATCCTTCAGGCGGGTACCAc > 1:2029983/1‑76 (MQ=255) aTAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCAc < 1:488991/76‑1 (MQ=255) aTAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCAc > 2:1204620/1‑76 (MQ=255) tAATGCGGAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACg < 1:636803/76‑1 (MQ=255) gAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTccc > 1:1179724/1‑76 (MQ=255) gAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTccc < 1:671124/76‑1 (MQ=255) gAACGTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTccc < 2:1178087/76‑1 (MQ=255) | GGGTTATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCC > REL606/3500563‑3500705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |