Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,923,350 T→A I291N (ATC→AAC)  rep → DNA helicase and single‑stranded DNA‑dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,923,3500TA100.0% 200.7 / NA 55I291N (ATC→AAC) repDNA helicase and single‑stranded DNA‑dependent ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (28/27);  total (28/27)

GCGCTGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTA  >  REL606/3923277‑3923423
                                                                         |                                                                         
gcgcTGAAGGTGATNAAGCTTGAGCAGAACTACCGCTCTTCCTGACGTATTTTGAAAGCGGCGGACATCCTGAACg                                                                         <  1:1743448/76‑1 (MQ=255)
gcgcTGAAGGTGAATAAGCTTGAGCAGAACTNTCGCTCTACCGGACGTATTTTGAAAGCGGCGANCATCCTGAACg                                                                         >  1:240204/1‑76 (MQ=255)
  gcTGAAGTTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGcc                                                                       <  1:437985/76‑1 (MQ=255)
    tGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCTGCGAACATCCTGAACGCCaa                                                                     >  1:2027122/1‑76 (MQ=255)
    tGAAGGCGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaa                                                                     >  1:1935673/1‑76 (MQ=255)
     gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAATATCCTGAACGCCaat                                                                    >  1:1692919/1‑76 (MQ=255)
     gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaat                                                                    <  1:980481/76‑1 (MQ=255)
     gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaat                                                                    >  2:1730200/1‑76 (MQ=255)
      aaGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaata                                                                   <  1:743528/76‑1 (MQ=255)
       aGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaataa                                                                  <  2:377035/76‑1 (MQ=255)
          tGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATcc                                                               <  1:1306592/76‑1 (MQ=255)
           gATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCg                                                              >  1:899251/1‑76 (MQ=255)
            aTTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGc                                                             <  2:943326/76‑1 (MQ=255)
              tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCTATAATCCGCAc                                                           >  2:700865/1‑76 (MQ=255)
              tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCAc                                                           <  1:1569982/76‑1 (MQ=255)
                  cTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCt                                                       <  1:269987/76‑1 (MQ=255)
                     gAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTg                                                    >  1:533971/1‑76 (MQ=255)
                      aGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGNCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGa                                                   <  1:1983657/76‑1 (MQ=255)
                        cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa                                                 >  2:151884/1‑76 (MQ=255)
                        cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa                                                 >  2:695706/1‑76 (MQ=255)
                        cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa                                                 <  2:1379469/76‑1 (MQ=255)
                          gAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAg                                               >  2:1378573/1‑76 (MQ=255)
                              tACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTc                                           >  2:487843/1‑76 (MQ=255)
                              gaCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTc                                           <  2:949940/75‑1 (MQ=255)
                               aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt                                          >  2:593907/1‑76 (MQ=255)
                               aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt                                          >  2:1432143/1‑76 (MQ=255)
                               aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt                                          <  1:1272007/76‑1 (MQ=255)
                               aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt                                          <  1:1066774/76‑1 (MQ=255)
                                ccGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTg                                         >  2:1335245/1‑76 (MQ=255)
                                 cGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGt                                        <  2:62550/76‑1 (MQ=255)
                                     ctTCCGGACGTATTTAGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTtctc                                    >  1:192094/1‑76 (MQ=255)
                                      ttCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTcc                                   >  1:694863/1‑76 (MQ=255)
                                       tCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGAGTCTGTTCTCCg                                  >  2:1764159/1‑76 (MQ=255)
                                        ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCTAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa                                 >  1:1704476/1‑76 (MQ=255)
                                        ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa                                 <  1:639631/76‑1 (MQ=255)
                                        ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa                                 <  1:777070/76‑1 (MQ=255)
                                        ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa                                 <  1:1539998/76‑1 (MQ=255)
                                         cGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGaa                                >  1:1392574/1‑76 (MQ=255)
                                         cGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTNCTCCGaa                                <  2:1643763/76‑1 (MQ=255)
                                              gTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTggg                           >  1:419465/1‑76 (MQ=255)
                                                 ttttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTa                        >  2:1226138/1‑76 (MQ=255)
                                                 ttttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTa                        <  1:818112/76‑1 (MQ=255)
                                                   ttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACg                      >  2:1775801/1‑76 (MQ=255)
                                                     gAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGc                    >  1:1580386/1‑76 (MQ=255)
                                                      aaaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                   <  1:1246290/76‑1 (MQ=255)
                                                      aaaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                   >  2:726659/1‑76 (MQ=255)
                                                       aaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcac                  <  1:593908/76‑1 (MQ=255)
                                                          cggcgAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACaga               <  2:464932/76‑1 (MQ=255)
                                                              gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa           <  2:1443313/76‑1 (MQ=255)
                                                              gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa           <  2:1051164/76‑1 (MQ=255)
                                                              gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa           <  1:868612/76‑1 (MQ=255)
                                                               aaCATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaa          >  2:1233030/1‑76 (MQ=255)
                                                                     cTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGttt    >  1:1627551/1‑76 (MQ=255)
                                                                      tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtttt   >  2:1286594/1‑76 (MQ=255)
                                                                      tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtttt   <  2:1109480/76‑1 (MQ=255)
                                                                      tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCC‑AACTGGGTTACGGCACAGAGCTAAAAGTTTTa  <  1:1899493/76‑1 (MQ=255)
                                                                         |                                                                         
GCGCTGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTA  >  REL606/3923277‑3923423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑