| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,165,277 | A→C | E106A (GAA→GCA) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,165,277 | 0 | A | C | 98.0% | 206.0 / NA | 52 | E106A (GAA→GCA) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (25/25); minor base G (0/1); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATAT > REL606/4165204‑4165349 | ggCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCAc > 1:349780/1‑76 (MQ=255) cATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCt > 1:656161/1‑76 (MQ=255) cATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCt < 2:1370177/76‑1 (MQ=255) tgtgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTccc > 1:1080669/1‑76 (MQ=255) gtgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTcccc > 2:1404195/1‑76 (MQ=255) tgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCg < 1:1325220/76‑1 (MQ=255) tgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCg < 2:636316/76‑1 (MQ=255) gagaAGTGCGGCGTTGAAGTGACCCTGAGTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGa < 1:324024/76‑1 (MQ=255) gagaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGa < 1:215835/76‑1 (MQ=255) agaAGTGCGGCGTTNAAGTGACCCAGACTAAAGTACGCCGTGTGCGTATGGGCCACATCGGACTGGCTTCCCCGAc < 1:1750708/76‑1 (MQ=255) agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGAc < 1:918885/76‑1 (MQ=255) tGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTgcgc < 1:975369/76‑1 (MQ=255) gcggcgTTGAAGTGACCCAGGCTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCATCCCCGACTGCGca > 1:98360/1‑76 (MQ=255) ggcgTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGcaca > 1:833147/1‑76 (MQ=255) ttGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTg > 1:905861/1‑76 (MQ=255) tGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTgg > 1:1449023/1‑76 (MQ=255) tGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTgg > 2:1336485/1‑76 (MQ=255) tGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCt < 1:438420/76‑1 (MQ=255) gACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTg < 1:976797/76‑1 (MQ=255) aCCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGa > 1:1645793/1‑76 (MQ=255) aCCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGa < 1:359296/76‑1 (MQ=255) cccAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGaa < 2:1509730/76‑1 (MQ=255) aGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATc < 1:979437/76‑1 (MQ=255) gACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCg < 2:689643/76‑1 (MQ=255) aCTAAAGTACGCCGTGAGCGTATGGGCTACAACGNACTGACTNACNCANCTGCTCACCTCTGGTTCCTGAAATCGc > 1:1759009/1‑76 (MQ=255) tAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGTGGAACTCTTGTTTCTGTCATCGcgt > 2:637416/1‑74 (MQ=255) tAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGGAAACGCTg > 2:637442/1‑76 (MQ=255) aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc > 2:587231/1‑76 (MQ=255) aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc > 2:1104228/1‑76 (MQ=255) aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc < 2:1760277/76‑1 (MQ=255) aaaGTACGCCGTGAGCGTACGGGCCACATCGNCCTGACGTCACCGACTGCGCACATCTGGTTCGNGAAATCGCTGc > 1:245287/1‑76 (MQ=255) aaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGcc < 1:1701237/76‑1 (MQ=255) aGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCg < 2:1037385/76‑1 (MQ=255) aGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCg < 2:1538203/76‑1 (MQ=255) gTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCCCTGCCGt > 1:949931/1‑76 (MQ=255) tACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTc < 1:1591069/76‑1 (MQ=255) tACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTc > 2:1238824/1‑76 (MQ=255) gCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCg > 1:724989/1‑76 (MQ=255) gCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCg > 1:1461963/1‑76 (MQ=255) gTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAt < 2:1771586/76‑1 (MQ=255) tGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATc > 1:550686/1‑76 (MQ=255) gAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCg < 1:968257/76‑1 (MQ=255) gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct < 2:1301546/76‑1 (MQ=255) gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct > 2:1293231/1‑76 (MQ=255) gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct > 1:1685071/1‑76 (MQ=255) gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAGCTGTct < 1:1914078/76‑1 (MQ=255) tATGGGCCACATCGCACTGGCTTCCNCGACTGCGCACATCTGGTTCTNGAATTCGCTGCCGTCCCGTATCGGTctg < 1:285663/76‑1 (MQ=255) tATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTctg < 1:1628968/76‑1 (MQ=255) tATGCGCCACATCGCACTGGCTTCACCGACTGCGCACATCTGGTTCCTGAAACCGCTGCCGTCCCGTATCGGTctg < 2:1454120/76‑1 (MQ=255) aTGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAGCGGGctgc > 2:1917815/1‑76 (MQ=255) tGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTctgct > 1:1806290/1‑76 (MQ=255) tGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGAATCGGTctgct > 2:1824136/1‑76 (MQ=255) gCCACATCGCGCTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTgctgct < 2:1841043/76‑1 (MQ=255) ccACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTc < 2:833146/76‑1 (MQ=255) caTCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGat > 1:171005/1‑76 (MQ=255) caTCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCCGCCTGCTGCTCGat > 2:499828/1‑76 (MQ=255) tCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGatat < 2:758197/76‑1 (MQ=255) | GGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATAT > REL606/4165204‑4165349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |