Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,262,746	C→A	A364S (GCT→TCT)	actP ←	acetate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,262,746	0	C	A	97.7%	140.3 / ‑2.4	44	A364S (GCT→TCT)	actP	acetate permease
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (23/19);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

TACAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGT  >  REL606/4262673‑4262817                                                                          |                                                                        tACAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCgaaagaaa                                                                       >  1:1472932/1‑76 (MQ=255)   cAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAAcc                                                                     >  2:1802416/1‑76 (MQ=255)   cAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAAcc                                                                     >  2:1606184/1‑76 (MQ=255)   cAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAAcc                                                                     >  2:1396322/1‑76 (MQ=255)     aGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGGAACCACCGCGAGGATAGTGGCGAAAGAAACCGc                                                                   >  1:1493055/1‑76 (MQ=255)           gagAACCCGCGGAGGCCCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAAt                                                             <  2:1116963/74‑1 (MQ=255)            cGAAACCGCGGATGCGCGCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATa                                                            >  1:2029679/1‑76 (MQ=255)             aaaaCCGCGGGGGCCCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATaa                                                           <  2:1907844/75‑1 (MQ=255)                  cgcgGATGCGCCCGCCAGCGCCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCg                                                      >  2:174063/1‑76 (MQ=255)                    cgGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAg                                                    >  1:842990/1‑76 (MQ=255)                    cgGACGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAg                                                    <  2:1017943/76‑1 (MQ=255)                      gATGCCCCCGCCAGTGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATCAAACCGAGGa                                                  <  1:163562/76‑1 (MQ=255)                       aTGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGaa                                                 >  2:1205477/1‑76 (MQ=255)                        tGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAAc                                                >  1:761520/1‑76 (MQ=255)                        ggcgcCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGNAAc                                                <  1:1832013/75‑1 (MQ=255)                        cgcgcCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAAc                                                <  2:948482/75‑1 (MQ=255)                         gcgcCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACa                                               >  1:1808994/1‑76 (MQ=255)                          cgcccgccAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAg                                              <  2:1879989/76‑1 (MQ=255)                            cccgccAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGt                                            >  2:1532152/1‑76 (MQ=255)                               gccAGCGTCAGACCCGCAACCACCGCGAGGATAGAGGCGAANGGAACCGCTGAAATAAAANCGNGGAACAGGTTgc                                         >  1:1663050/1‑76 (MQ=255)                                ccAGCGTCAGACCCTCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAACCCGAGGTACAGGTTgcc                                        <  1:174063/76‑1 (MQ=255)                                  aGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTgccgc                                      >  1:1329004/1‑76 (MQ=255)                                  aGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTgccgc                                      >  2:1327567/1‑76 (MQ=255)                                   gCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTgccgcc                                     <  1:146844/76‑1 (MQ=255)                                     gTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCa                                   >  1:1084613/1‑76 (MQ=255)                                      tCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCAc                                  <  1:473233/76‑1 (MQ=255)                                       cAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACt                                 >  1:1599933/1‑76 (MQ=255)                                       cAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACt                                 >  2:1416964/1‑76 (MQ=255)                                         gACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGc                               <  1:364782/76‑1 (MQ=255)                                         gACCCGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGc                               <  1:858413/76‑1 (MQ=255)                                             cGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTc                           <  1:961653/76‑1 (MQ=255)                                             cGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTc                           >  1:1312034/1‑76 (MQ=255)                                             cGCAACCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTc                           >  2:1236447/1‑76 (MQ=255)                                                aaCCACCGCGAGTATCGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGNCCACTGCATTCGcc                        >  1:1752298/1‑76 (MQ=255)                                                aaCCACCGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGcc                        >  2:693440/1‑76 (MQ=255)                                                 accaccGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAGCTCGAGGCACTGGCTGCCGCCCCCTGNATTCGCCa                       >  2:331018/1‑76 (MQ=255)                                                 accaccGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCa                       <  2:1289548/76‑1 (MQ=255)                                                     ccGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTg                   <  2:761294/76‑1 (MQ=255)                                                     ccGCGAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTg                   <  2:864217/76‑1 (MQ=255)                                                        cgAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACTGAAGAACAGGTNGCCGCCCACTGCATTCGCCAGGTGAAc                <  1:1778974/76‑1 (MQ=255)                                                        cgAGGATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAAc                <  1:972941/76‑1 (MQ=255)                                                            gATAGTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGgcc            <  2:634826/76‑1 (MQ=255)                                                                gTGGCGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCa        <  2:1126492/76‑1 (MQ=255)                                                                    cGAAAGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCAtgtt    >  2:413740/1‑76 (MQ=255)                                                                      aaaGAAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCAtgttgt  >  2:446579/1‑76 (MQ=255)                                                                      aaaGAAACAGCTGAAATAAAACCGAGGAACAGGTTCCCGCCCACTGAATTCGCCAGATGAAGGGCCGCCAtgttgt  >  2:45753/1‑76 (MQ=255)                                                                          |                                                                        TACAAGTCATGCGAAACCGCGGATGCGCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGT  >  REL606/4262673‑4262817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑