Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,164,913	G→A	T310M (ACG→ATG)	yehU ←	predicted sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,164,913	0	G	A	100.0%	202.2 / NA	56	T310M (ACG→ATG)	yehU	predicted sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/32);  total (24/32)

CGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACC  >  REL606/2164841‑2164981                                                                         |                                                                     cGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCTCGAGCTGATTTNCGCCACGCAACGGAATTACCAGCATCg                                                                   <  1:1783801/76‑1 (MQ=255) cGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCg                                                                   <  1:609775/76‑1 (MQ=255)    tttttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGAcc                                                                >  2:1202892/1‑76 (MQ=255)      tttGGCATCGTACCATTTCATGGTGCCCATCAGGCGCTGATTTTCGCCACGCAACGGAATANCCNGCACCGAcccc                                                              >  2:321886/1‑76 (MQ=255)       ttGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCa                                                             >  2:1903820/1‑76 (MQ=255)        tGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAg                                                            <  2:1589304/76‑1 (MQ=255)         ggCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGt                                                           <  1:795558/76‑1 (MQ=255)         ggCTTCATACAATTTGATGGTACCAACCAAGCGCCCATTATCGCCACGCAGCGGTATTACGAGCATCGCCCCCAGt                                                           >  1:2035185/1‑76 (MQ=255)         ggCTTCATACAATTTGATGGAGCCGATCACGCGCTGATTTTCGCCACGCAACGGAACTCCCTGCATCGACCCCAGt                                                           >  1:94795/1‑76 (MQ=255)          gCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGtt                                                          >  2:110237/1‑76 (MQ=255)            ttCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTg                                                        <  1:789463/76‑1 (MQ=255)             tCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGc                                                       >  2:1403133/1‑76 (MQ=255)              cATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCa                                                      >  2:256936/1‑76 (MQ=255)               aTACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCAt                                                     >  1:1002268/1‑76 (MQ=255)                  cAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGc                                                  <  1:1229783/76‑1 (MQ=255)                    aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCgg                                                >  1:1224893/1‑76 (MQ=255)                    aTTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCgg                                                >  1:1279507/1‑76 (MQ=255)                      ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGAt                                              >  2:441605/1‑76 (MQ=255)                      ttGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGAt                                              >  2:1369033/1‑76 (MQ=255)                       tGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATg                                             >  1:929855/1‑76 (MQ=255)                       tGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATg                                             >  2:389505/1‑76 (MQ=255)                        gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGc                                            <  2:388677/76‑1 (MQ=255)                        gATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGc                                            >  1:536238/1‑76 (MQ=255)                         aTGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCa                                           <  1:1935846/76‑1 (MQ=255)                         aTGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTACGGATGCa                                           <  1:1891140/76‑1 (MQ=255)                           ggTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATAGACCCCAGTTTGCATTGCGGATGCaaa                                         <  1:204393/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACTCCAGTTTGCNTTGCGGATGCAAag                                        <  1:4430/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAag                                        <  2:484886/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAag                                        <  2:1719398/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAag                                        <  2:162552/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAag                                        <  2:681018/76‑1 (MQ=255)                            gTGCCCATCACGCGCTGATNTNCGCCACGNAACGGAATTACCACCATCGACNCCNGTGTGCATTGCGGATGCAAag                                        <  2:298350/76‑1 (MQ=255)                              gCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTCCCAGCATCGACCCCAGTTTGCATTGCGGATGCAAagag                                      >  1:1707871/1‑76 (MQ=255)                               cccATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGc                                     <  1:1159325/76‑1 (MQ=255)                                 cATCACGCGATGAATTTCGCCACGCAACGGANCTACGAGAACCGACCCCAGTTTGCATTGCGGANGCAAAGAGCaa                                   >  1:239883/1‑76 (MQ=255)                                   tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCANTTTTCATTGCGGATGCAAAGAGCAACg                                 <  2:3711/76‑1 (MQ=255)                                   tCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACg                                 <  2:1797770/76‑1 (MQ=255)                                    cACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGa                                >  1:565156/1‑76 (MQ=255)                                      cgcgCTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATCGCGGATGCAAAGAGCAACGATa                              >  2:159663/1‑76 (MQ=255)                                       gcgcTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGGAACGATaa                             <  2:1454095/76‑1 (MQ=255)                                       gcgcTGATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATaa                             >  2:1514922/1‑76 (MQ=255)                                         gcTGATCTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAgg                           >  1:1865941/1‑76 (MQ=255)                                            gATTTTCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTAc                        >  2:1718123/1‑76 (MQ=255)                                            gATTTTCGACACGCAACGGAATTACCACCATATACCCTAGTTGGCACTGCGGATGCGAAGTGGCACGATAAGGTAc                        <  1:1967900/76‑1 (MQ=255)                                                 tCGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGt                   <  1:1548374/76‑1 (MQ=255)                                                  cGCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGtt                  >  2:1399312/1‑76 (MQ=255)                                                   gCCACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTg                 <  2:445781/76‑1 (MQ=255)                                                    ccACGCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGc                >  2:1711425/1‑76 (MQ=255)                                                      aCGCAACGGAATTACCAGCATCGACCCCAGTTTGCACTGCGGATGCAANGAGCAACGATAAGGTACTTCGTTGCCa              <  1:1796638/76‑1 (MQ=255)                                                        gCAACGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCNTTGCCATc            <  1:1953722/76‑1 (MQ=255)                                                          aaCGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg          <  2:1096012/76‑1 (MQ=255)                                                          aaCGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg          <  1:1328308/76‑1 (MQ=255)                                                          aaCGGAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAg          <  1:742542/76‑1 (MQ=255)                                                             ggAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGt       <  1:748032/76‑1 (MQ=255)                                                             ggAATTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGt       <  2:1255930/76‑1 (MQ=255)                                                             ggAATTACCAGCATCGACCCCACTTTGNGTNGGNGAGGCAANGAGCAATGATAAGGTACTTCGTTGCCATCAGCGt       <  1:1770822/76‑1 (MQ=255)                                                               aaTTACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAg     <  1:1445967/76‑1 (MQ=255)                                                                  tACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc  <  2:1135008/76‑1 (MQ=255)                                                                  tACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc  <  1:1843319/76‑1 (MQ=255)                                                                  tACCAGCATCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGAcc  >  2:1893623/1‑76 (MQ=255)                                                                         |                                                                     CGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACGCGCTGATTTTCGCCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGATAAGGTACTTCGTTGCCATCAGCGTAGACC  >  REL606/2164841‑2164981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑