| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 36.0% | 306.1 / 56.5 | 179 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (28/86); new base G (64/0); total (93/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 266 total aligned reads displayed.
CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 | ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtggg > 1:1015816/1‑75 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACACTGCCGCCATCAGAATCGACTCCAGCCTACGGCA‑ggtagg > 2:5686837/1‑73 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggggggg > 1:4305285/1‑74 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑Ggtggtg < 1:6569781/76‑1 (MQ=255) aGATGAGGCGAACTGTAGGCGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGc < 1:5773581/76‑1 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCCGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGc < 1:1841889/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCtt > 1:2906679/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCtt > 1:951049/1‑76 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTg < 1:7229218/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTg > 1:5376975/1‑76 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGCATCGACTCCAGCGTACGGCC‑GGTGGTGGCttttc > 2:3886443/1‑74 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCa < 1:205224/76‑1 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCAt > 1:7260092/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCAtt < 1:886556/76‑1 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCCtt > 1:6703095/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCCTGCCAtt > 2:6659484/1‑76 (MQ=255) aaCTGTACCCGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCAtt < 2:5403024/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTc < 2:6462793/76‑1 (MQ=255) tgtgGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGt < 1:4543/76‑1 (MQ=255) tggaGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGt < 2:283643/73‑1 (MQ=255) tGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGt < 1:5681239/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGt < 1:3761001/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGGCATTcct > 1:8154445/1‑74 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCCTTGCCATTCGTa > 1:1277958/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTa > 1:1952039/1‑76 (MQ=255) tAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAc < 1:8490887/76‑1 (MQ=255) gaGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAc < 1:5879454/75‑1 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGGCTTTGCCATTCGTAcc > 1:4007925/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGTCAATCGGAcc > 1:3312129/1‑76 (MQ=255) ccGCCCCTTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCg < 2:1749975/75‑1 (MQ=255) aCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGGCCTTTGCCATTCGTACCCg > 1:6927918/1‑76 (MQ=255) cGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTTGTAACCGc > 2:3124984/1‑76 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCATTCGTACCCGCa > 1:2439763/1‑76 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGGCCTTCGTACCCCCa > 1:5194343/1‑76 (MQ=255) ccccCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCaa < 1:3669137/76‑1 (MQ=255) cctcTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAAc < 1:7742508/73‑1 (MQ=255) ccACTTTTTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAAc < 1:5292765/76‑1 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCCAc > 1:3203006/1‑76 (MQ=255) cccTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACg < 2:2439311/74‑1 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCATTCGTAACCGCAACggg > 1:2322668/1‑75 (MQ=255) ttGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaa < 1:3049622/76‑1 (MQ=255) tGTCCCCTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa < 2:841630/76‑1 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTGGCCATCCG‑ACCCGCAACGGTaaa > 1:1312407/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCCACGGGAAac > 2:6059495/1‑76 (MQ=255) tcccccGCGGCAATCAGAATCGGCACCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 2:5258188/74‑1 (MQ=255) tccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 2:1860046/74‑1 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCATTCGTAGCCGCCACGGTAAaca > 2:7553950/1‑76 (MQ=255) tACCCTGCCACCATCAGAATCGAATCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 2:8958216/76‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAaccc > 1:1627402/1‑74 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGCGCCCTTGCCATTCGTACCCCCAACGGGAAAcaca > 1:7479433/1‑76 (MQ=255) ccTCCCGCCATCAGATTCGCCTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 2:1571629/76‑1 (MQ=255) cgccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:2409863/75‑1 (MQ=255) cgccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 1:6561831/75‑1 (MQ=255) ccccgccATCAGAACCGACTCCAGCGTACGGCA‑GGTGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 1:4962245/74‑1 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATgg > 1:4442720/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAAAGGGAAACACAAATGgcgc > 1:7138048/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt < 1:1112644/76‑1 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTAACCGCAACGGTAAACAACAATGGCGCTg > 2:2571352/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTGGt > 2:1090476/1‑76 (MQ=255) aGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGtt < 1:7887425/76‑1 (MQ=255) aGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTTCCATTCGTACCCGCAACGGGAAACACAAATAGCGCTgggt > 1:7057659/1‑74 (MQ=255) gAATCGCCCCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:4473891/76‑1 (MQ=255) aaaTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 1:6196177/75‑1 (MQ=255) aaTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 1:6022596/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc > 2:8502571/1‑76 (MQ=255) aTCGCCCCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:6820987/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:6350618/76‑1 (MQ=255) cgccTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:7649109/73‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:7127488/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:6755488/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCCTACCCGCAACCGGAAACACAAAACGCGCCGGGTTCAgg > 1:6991397/1‑76 (MQ=255) gACCCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 1:2519545/76‑1 (MQ=255) ccTCCAGCGTACGGCG‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGAc < 1:5164606/75‑1 (MQ=255) ccTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGAc < 1:8825375/75‑1 (MQ=255) tCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGCAAACACAAATGGCGCTGGGTTTAGGACGc > 2:2164455/1‑76 (MQ=255) tCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGc > 1:5460503/1‑76 (MQ=255) aGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 2:4587239/76‑1 (MQ=255) gCGTACGGCG‑GGGGGTGCCTTTCCCATTCGTCCCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:7658436/76‑1 (MQ=255) gCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:6795793/76‑1 (MQ=255) cGTACGGCA‑GGGGGGGGCTTTGCCATTCGTACCCCCCACGGTAAACACCAATGGCGCTGGTTTCAGGACGCCAAGa > 2:4236646/1‑76 (MQ=255) ggaCGGCA‑GGGGGGGGCTTTTACACTTGTACGCGCCACGGCTAACCCAAATAGGGCTGGATTTAGGACGCCAAGAc > 2:4933045/3‑76 (MQ=255) tACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 2:686488/76‑1 (MQ=255) cGGCA‑GGGGGTCCCTTCGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg < 1:6210257/76‑1 (MQ=255) ggCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 1:2168876/1‑76 (MQ=255) ggCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 1:8064670/1‑76 (MQ=255) ggCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 1:2564225/1‑76 (MQ=255) cA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:5111196/1‑76 (MQ=255) cA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg < 2:796067/76‑1 (MQ=255) cA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 2:8848807/1‑76 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:7521765/1‑76 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGATTCAGGACGCCAAGACGggccgc > 1:3504210/1‑76 (MQ=255) ggtggtGCTTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 1:2653031/76‑1 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 2:3694050/76‑1 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 1:7470775/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 2:7820347/1‑76 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa > 1:2879114/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCCGGACGCCAAGACGCGCCGCGAc > 1:4769425/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:6335248/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:8140467/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:1261636/1‑76 (MQ=255) | CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |