| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,535,009 | 0 | A | C | 36.3% | 288.1 / 24.4 | 146 | T86P (ACC→CCC) | focB | predicted formate transporter |
| Reads supporting (aligned to +/- strand): ref base A (71/22); new base C (0/53); total (71/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 211 total aligned reads displayed.
TCTTCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGA > REL606/2534934‑2535084 | cTTCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCAc < 1:4978503/76‑1 (MQ=255) cTTCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCAc > 1:2074163/1‑76 (MQ=255) tCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCa < 2:4152721/76‑1 (MQ=255) cacaGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCAcccg > 1:1881027/1‑74 (MQ=255) cacaGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAg < 2:1148521/76‑1 (MQ=255) acaGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGGTTGCGGGACCAGc > 2:7500641/1‑76 (MQ=255) caGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCCCTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGcc < 2:5658429/76‑1 (MQ=255) caGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGcc > 1:353473/1‑76 (MQ=255) ggCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTg < 1:6257831/76‑1 (MQ=255) tAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCAc > 1:7324281/1‑76 (MQ=255) aaCCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTTCGGCACCAGCCTGTTCAcc > 2:8389629/1‑76 (MQ=255) aCCCATCTGGGGGGCGCCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCt < 2:4811191/76‑1 (MQ=255) cccATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCtc > 1:1315883/1‑76 (MQ=255) ccATCTGGTGGGCGGCCTTTGCTTTGCCCTCGGCTTTATTTGGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCtcg < 1:6084792/76‑1 (MQ=255) ccATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCtcg > 1:4196787/1‑76 (MQ=255) cATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCtcgt < 1:401573/76‑1 (MQ=255) agcTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCGGGCGGTTTGCGGCCCCAGCCTGTTCACCtcgtc < 1:7858015/74‑1 (MQ=255) aTCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCtcgtc > 2:8505186/1‑76 (MQ=255) cTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGGACCAGCCTGTTCACCTCGTCgg > 1:239378/1‑76 (MQ=255) cTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCGCCAGCCTGCTGACCTTGCCgg > 1:5980078/1‑76 (MQ=255) cTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCgg > 1:6653532/1‑76 (MQ=255) tgggggGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGt < 1:5872625/76‑1 (MQ=255) ggtggGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTa < 1:8636620/76‑1 (MQ=255) tggGCGTCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAAt < 1:305846/76‑1 (MQ=255) tggGCGTCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAAt < 2:6789012/76‑1 (MQ=255) ggggCGTCCTTTGCTTTGCACTCGGTTTTATTTTGCGGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAAt < 2:405179/75‑1 (MQ=255) ggggCGGCCTTTGCTTTGCCCTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAAt < 1:683468/75‑1 (MQ=255) gggCGGCCTTTGCTTTGCCCTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATg < 2:5459966/76‑1 (MQ=255) cggcCTTTGCTTTGCCCTCGGCTTTATTTTGCGGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACg < 1:3842873/76‑1 (MQ=255) cggcCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACg < 1:8068609/76‑1 (MQ=255) cggcCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACg < 1:3329416/76‑1 (MQ=255) cggcCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACg < 1:5315435/76‑1 (MQ=255) cggcCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACg > 1:6553655/1‑76 (MQ=255) cggcCTTTGCTTTCCCCTCGGCTTTATTTGGGTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACg < 1:7450899/76‑1 (MQ=255) ccttttgttttccACTGGGTTTTTTTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGt < 1:7927500/73‑1 (MQ=255) cttttgcttcacCCTCGGTTTTATTTTGATGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTg < 1:312205/74‑1 (MQ=255) ctttgctttgCTCTCGGTTTTATTTTGCTGGCGATTGGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGa < 2:2185244/76‑1 (MQ=255) tttgctttgcCCTCGGCTTTTTTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGAt < 1:4873184/76‑1 (MQ=255) tttgctttgcACTCGGGTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGAt < 1:6619768/76‑1 (MQ=255) ttgttttgcACTCGGCTTTATTTTGCTGGCGTTTTGCGCCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATg < 1:5089955/76‑1 (MQ=255) ttgctttgctCTCGGTTTTTTTTGGCGGGGGGTTGGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATg < 1:6779768/76‑1 (MQ=255) ttgctttgcCCTCGTCTTTATTTTGCGGGCGTTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATg < 1:715941/76‑1 (MQ=255) ttgctttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCCGCCTGTTCACCTCGTCGGTAATGACGGTGATg > 2:1438498/1‑76 (MQ=255) ttgctttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATg > 1:2652316/1‑76 (MQ=255) ttgctttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCAACTCGTCGGTAATGACGGGGATg > 2:8113398/1‑76 (MQ=255) tgctttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGGGATgg > 2:3722852/1‑76 (MQ=255) gctttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGGGATGGc > 2:8810310/1‑76 (MQ=255) cttttcACTCGGCTTTTTTTTGCGGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCa < 1:6335245/76‑1 (MQ=255) ctttccACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCa < 2:5071550/76‑1 (MQ=255) tttgcACTGGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCaa < 2:4454811/76‑1 (MQ=255) tttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCaa > 1:8977581/1‑76 (MQ=255) ttgcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCaaa > 1:6966991/1‑76 (MQ=255) gcACTCGGCTTTATTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCaaaaa < 2:3317238/76‑1 (MQ=255) cACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCCCGTCGGTAATGACGGTGATAGCAAAAAg > 1:184947/1‑76 (MQ=255) cTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTTACCTCGTCGGTAATTACGGAGATGGGAAAAAgac > 1:4504194/1‑74 (MQ=255) cTCGGCTTTATTTTGCGGGGGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTc < 1:7663360/76‑1 (MQ=255) cGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGGTGGCGAAAAGACgg > 2:255342/1‑76 (MQ=255) ggCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGGGATGGCAAAAAGTCggg > 1:2920192/1‑76 (MQ=255) ggCTTTATTGGTCGGGGGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCggg < 1:2955162/76‑1 (MQ=255) cTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGc > 1:8888062/1‑76 (MQ=255) cTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAAGCGGGGc > 2:1845689/1‑76 (MQ=255) cTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGGAATGACGGGGATGGCAAAAAGGCGGGGc > 2:3219324/1‑76 (MQ=255) tttATTTTGCTGGCGGGTTGCGGCACCAGCCTGTTCAACTCGTCGGTAATGACGGTGATGGCAACAAGTCgggggg > 2:7583201/1‑74 (MQ=255) ttATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGt > 1:4042722/1‑76 (MQ=255) tATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGtt > 1:6386916/1‑76 (MQ=255) aTTTTGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGtta < 2:5492867/76‑1 (MQ=255) aTTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttt > 1:5398169/1‑75 (MQ=255) tttGGTGGGGTTTCGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatt < 1:4922664/76‑1 (MQ=255) tttGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatt > 1:1627532/1‑76 (MQ=255) tttGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatt < 2:2319057/76‑1 (MQ=255) ttGCTGGCGTTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatta < 1:1960422/76‑1 (MQ=255) ttGCTGGCGGTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatta < 1:467234/76‑1 (MQ=255) ttGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGGGATGGCAAAAAGTCGGGGGGttatta > 1:321205/1‑76 (MQ=255) ttGCGGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatta < 2:733835/76‑1 (MQ=255) ttGCGGGCGGTTTGCCGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatta < 2:8538657/76‑1 (MQ=255) tggCTGGGGTTTTGCGCCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGttatta < 1:5728852/74‑1 (MQ=255) tGCTGGGGGTTTGGGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAg < 2:4373143/76‑1 (MQ=255) tGCTGGCGTTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAg < 1:8605875/76‑1 (MQ=255) tGCGGGCGTTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAg < 1:6033527/76‑1 (MQ=255) gCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGGGATGGCAAAAAGTCGGGGCGGTTTTAGt > 2:3448333/1‑76 (MQ=255) cTGGCGTTTTGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGtt < 2:5533593/76‑1 (MQ=255) cTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGtt > 1:4323434/1‑76 (MQ=255) cTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGGTATTAGtt > 2:2436166/1‑76 (MQ=255) tGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGGTATTAGTTg > 2:694977/1‑76 (MQ=255) tGGCGGATTGCGGCGCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTg < 2:1515670/76‑1 (MQ=255) ggCGTTTGGCGGCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTgg < 1:5424341/76‑1 (MQ=255) gCGGTGTGCGACCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATAGCAAAAAGTCGGGGCGTTATTAGTTGGc < 2:6175950/76‑1 (MQ=255) gggTTTGCGACCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCg < 1:3977007/75‑1 (MQ=255) cgttttGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTCATTAGTTGGCg < 2:229817/73‑1 (MQ=255) cGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCg > 1:2537208/1‑76 (MQ=255) cGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCg > 1:2124802/1‑76 (MQ=255) ggTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGa > 1:3297455/1‑76 (MQ=255) ggTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGa > 1:3435376/1‑76 (MQ=255) gTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGaa > 1:6181643/1‑76 (MQ=255) gTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGaa > 2:818669/1‑76 (MQ=255) tttGCGCCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAAc < 1:2732207/76‑1 (MQ=255) tGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACtt > 1:6593450/1‑76 (MQ=255) tGCGCCCCCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACtt < 1:4799787/76‑1 (MQ=255) ggcgcCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACtt < 2:576749/75‑1 (MQ=255) gCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTg > 1:763866/1‑76 (MQ=255) | TCTTCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTGCACTCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGA > REL606/2534934‑2535084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |