| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,344,466 | 0 | T | G | 36.1% | 499.0 / 108.6 | 263 | F515V (TTT→GTT) | acrF | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (33/135); new base G (95/0); total (128/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-43 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 345 total aligned reads displayed.
CCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGAT > REL606/3344391‑3344541 | tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGttt > 1:3711256/1‑76 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGttt < 2:4101642/76‑1 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGgtt > 2:6973859/1‑76 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGgtt > 1:4159205/1‑76 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGgtt > 1:280642/1‑76 (MQ=255) gCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTa < 2:1218037/76‑1 (MQ=255) gCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTa < 1:7460770/76‑1 (MQ=255) gCGTTATGTGCAACGCGGCTTAAGCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTa < 1:522483/76‑1 (MQ=255) cGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTaa > 2:4934773/1‑76 (MQ=255) ttATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTtata > 1:2997356/1‑76 (MQ=255) aTGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATacc < 2:8349292/76‑1 (MQ=255) gCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCt > 1:6318670/1‑76 (MQ=255) cAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATaacaccct > 2:4735553/1‑74 (MQ=255) aCGATGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCg < 1:5005516/76‑1 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGAt < 1:4333219/76‑1 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGAt < 1:1449162/76‑1 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGAt < 1:3443009/76‑1 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGAt > 1:8251903/1‑76 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATAACACCCTCTAt > 2:3326866/1‑76 (MQ=255) ttAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATaa > 1:5611824/1‑75 (MQ=255) ttAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAg > 1:575618/1‑76 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGc < 2:1595176/76‑1 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGc < 1:4506178/76‑1 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCCTAGc > 1:663051/1‑76 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGc > 1:464241/1‑76 (MQ=255) aaaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACAACCTTAGATCATAGCg > 2:8645010/1‑76 (MQ=255) aaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGt < 1:1549483/76‑1 (MQ=255) aaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGt < 1:1981127/76‑1 (MQ=255) aaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCCCCTTCGTTTATAGCGt > 1:6360043/1‑76 (MQ=255) cccGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTa > 2:5683270/1‑76 (MQ=255) ccGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTaa < 2:8815530/76‑1 (MQ=255) cGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑c < 1:2593648/76‑1 (MQ=255) cGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGTTAA‑c > 2:3253497/1‑76 (MQ=255) cGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCCATCATCACGTTAA‑c > 2:4398614/1‑76 (MQ=255) gTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑cc < 2:3481493/76‑1 (MQ=255) gTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGTTAA‑cc > 2:712787/1‑76 (MQ=255) ctctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCAc > 1:7199893/1‑76 (MQ=255) ctctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCCTCGGTCCTAGCGTTca‑ccac > 1:2771728/1‑76 (MQ=255) ctctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCCTCGATCATAGCGTTAA‑CCAc > 1:5821716/1‑76 (MQ=255) ctgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTa < 1:8907144/76‑1 (MQ=255) ctgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCCCCCTCGATCATATCGTTAA‑ACACTa > 2:6804757/1‑76 (MQ=255) ctgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTa > 1:4303735/1‑76 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTac > 2:4289574/1‑76 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCCGGCACAGCGTTTA‑CCACTac > 2:1756893/1‑76 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCCTCGATCATAGAGTTAA‑CCCCTac > 1:5342793/1‑76 (MQ=255) ctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTacac < 2:5172148/76‑1 (MQ=255) ctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGTTAA‑CCACTacac > 2:4206866/1‑76 (MQ=255) ctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTccac > 1:6268652/1‑76 (MQ=255) tgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACAcc < 1:1483190/76‑1 (MQ=255) tgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATAACACCTTCGATCATAGCGTTAA‑ACACTACAcc > 1:1659232/1‑76 (MQ=255) gAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCa < 1:1247162/76‑1 (MQ=255) gAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCa < 1:3579972/76‑1 (MQ=255) cATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACa < 2:2772343/76‑1 (MQ=255) cATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACa > 1:6959715/1‑76 (MQ=255) cACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCCCTACACCAACAGCg > 1:7619752/1‑76 (MQ=255) aCGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCTTCGATCATAGCGGTAA‑CCACTACACCAACAGCgc > 1:2421461/1‑75 (MQ=255) cGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTc < 2:5630861/76‑1 (MQ=255) aaaTAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTTA‑CCCCTACACCAAAAGCGTCGGc > 2:8505099/1‑76 (MQ=255) aaTAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCa < 2:7388861/76‑1 (MQ=255) aaTAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCCTCGGTCATAGCGTTAA‑ACACTACACCCACACCGGCGGCa > 2:3364133/1‑76 (MQ=255) aTAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCgggca > 1:8073503/1‑73 (MQ=255) aTAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCTAGCATAGCGGTTA‑ACACTACCCCAACAGCGTCGGcca > 2:8005156/1‑74 (MQ=255) aTAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGcca > 2:5876959/1‑74 (MQ=255) tAAGGGCGGTTTCTTCGGCTGGGTTTATCCCACCTTCGCTCATATCGCTAAGCCAATAC‑CCATCAGCGCCGGccga > 2:2464850/1‑73 (MQ=255) tAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCaaa > 1:5887591/1‑76 (MQ=255) aaGGGCGGTTTCAACGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCaaaa < 1:8759550/76‑1 (MQ=255) gggCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATc < 1:3164175/76‑1 (MQ=255) cGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTc < 2:2243236/76‑1 (MQ=255) ggTTTCTTCGGCTGGTTTAATGCCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCg < 2:1001162/76‑1 (MQ=255) gTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAAACCTCgg > 2:2382832/1‑76 (MQ=255) tttCTTCGGCTGGTTTAATACCCCCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGt < 2:656687/76‑1 (MQ=255) ttcttcGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGGAAAATCCTCGGtt > 2:6422190/1‑76 (MQ=255) ttcttcGGCTGGGTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAAACCTCGGtt > 2:1925437/1‑76 (MQ=255) tcttcGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTc < 2:3845666/76‑1 (MQ=255) cttcGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTcc < 2:6080694/76‑1 (MQ=255) cGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCcaca < 2:2839113/76‑1 (MQ=255) ggCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAg < 1:8844305/76‑1 (MQ=255) ggCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGGTCCACAg > 1:3352467/1‑76 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 2:1413576/76‑1 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 1:2157150/76‑1 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg > 1:1017133/1‑76 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg > 1:6687941/1‑76 (MQ=255) cgggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGa < 2:7296905/74‑1 (MQ=255) cTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGa > 2:7720565/1‑76 (MQ=255) tGGTTTAATCCCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc < 1:6437561/76‑1 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc < 2:4829246/76‑1 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 2:7960892/1‑76 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 2:2687357/1‑76 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 1:1913204/1‑76 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg > 1:7333776/1‑76 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg < 1:7786668/76‑1 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg < 2:4460755/76‑1 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg > 2:7792755/1‑76 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTCCCACAGGACg > 1:1148596/1‑76 (MQ=255) gTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGa < 2:5987978/76‑1 (MQ=255) gTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGa > 2:4548374/1‑76 (MQ=255) tttAATCCCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGat < 1:853946/76‑1 (MQ=255) tttAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGat > 1:4929075/1‑76 (MQ=255) tttAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGat > 2:3128905/1‑76 (MQ=255) tttAATACCACCTTCGATCATAGCGTTAA‑CCACTACAACAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGat > 1:4599035/1‑76 (MQ=255) | CCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAA‑CCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGAT > REL606/3344391‑3344541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |