| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 37.1% | 341.9 / 193.1 | 221 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (4/135); new base G (82/0); total (86/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 280 total aligned reads displayed.
CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 | cGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggctggc < 1:6935750/76‑1 (MQ=255) aaTGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGtc‑t < 2:799914/76‑1 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑Tccc < 1:1134417/76‑1 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑Tccc < 2:4198616/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCa < 2:2142492/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCa < 2:2022358/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCa < 1:8416116/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCAt < 1:4885369/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCAt < 2:8613057/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCAt < 2:8275602/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCAt < 2:1094097/76‑1 (MQ=255) aaaaaTATCGGTATAACAGAACATCATATATAACATTCCGTTATCGAAAATAATGTGGCTTGCGGAGTA‑TCCAATg < 2:4394984/76‑1 (MQ=255) aaaaaTATCGGTATAAAATAGCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATg < 2:8489120/76‑1 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑Tcccctc > 2:3823491/1‑73 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATg < 2:5779963/76‑1 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGG‑TCCCCTGccc > 2:3148238/1‑75 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCATGCCa > 1:6251445/1‑76 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATa < 1:8248262/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATa < 1:3615651/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATa < 2:4595040/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGG‑TCCCCTGCCCTaa > 1:2140039/1‑76 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCATaa > 2:645025/1‑76 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATaa < 1:8034708/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATaa < 1:6465435/76‑1 (MQ=255) atCGGTATAAAAGAACAAAATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATaa < 1:8795453/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAAc < 1:7299774/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAAcc < 1:5589660/76‑1 (MQ=255) gTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTTGCTGGCGGCGTC‑TCCCATGCCATAACC‑aa < 2:4927754/76‑1 (MQ=255) gTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCATGCCCTAACC‑aa > 1:2821196/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCacaaac‑aaa > 1:3092705/1‑75 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCCTAACC‑AAt > 1:5854863/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGGGCC‑TCCCCTGCCACAAcccca > 2:2458839/1‑73 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGG‑TCCCCTTCCCTAAAC‑aact > 1:1497665/1‑74 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTCCCCTAAAC‑AAtt > 1:8749721/1‑76 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTTCCCCTGCCCTACCA‑AAtt > 2:6249533/1‑76 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCCCAAAC‑AAttt > 2:8101627/1‑76 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AAttt < 2:385572/76‑1 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCCGCCATAACA‑AAattt > 1:71383/1‑76 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AAtttt < 2:917608/76‑1 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCtt < 1:4969966/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCttt < 1:8150593/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTa < 1:7077330/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTa < 2:1792153/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTa < 2:1898325/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTa < 2:4138907/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTa < 1:483473/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCATAACC‑AAATTTTTCttttt > 1:3353400/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTCCCCTAACC‑AATTTTCTCtttta > 1:526193/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGGGGC‑CCCCCCGCCCAAAAC‑CATTTTCTttttta > 1:8636509/1‑74 (MQ=255) tcatATATAACATTACGTTATCGAAAATAAGGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAAc < 2:2669685/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGCCGCCCCCTCCCAAAACA‑AATTTCTTCTTTaatg > 2:4771986/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACgg < 1:2344492/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACgg < 2:7698911/76‑1 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGa < 2:8748327/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGG‑CCCCATGCCCTAACC‑AATTTTCTCTTTTACGGac > 2:4286466/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGac < 2:7183335/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCATTAAC‑AATTTTTTTTTTTAAGGaca > 1:8501053/1‑76 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGaca < 1:1613833/76‑1 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGacaa < 1:169778/76‑1 (MQ=255) taACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGacaac < 1:5509320/76‑1 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGCGGC‑GCCCCTAGCAAAAAC‑AAATTTCTCTTTTAAGGaaaaca > 2:2966525/1‑76 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACt < 1:7728280/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGGGGGGTC‑TCCCCTGCCCTAAAC‑AAATTTCTTTTTTACGGACAACACtt > 1:3739113/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACtt < 1:5474419/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACtt < 1:7811310/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACtt < 2:3391695/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTc < 1:1007484/76‑1 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCCGCCCTAACC‑CATTTTTTCTTTTAAGGACAACAcctccctc > 2:5349695/1‑76 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCATGCCATAACC‑AAATTTTTCTTTTACGGACAACACCTCActc > 1:991775/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGG‑TCCCCTGCCCTAACC‑AAATTTTTCTTTAACCGACAACACTTTTctct > 2:1302999/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCCTAAAC‑AAATTTTTCTTTTACGGACAAAACTTTCctct > 2:6864857/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCATAACC‑AAATTTTTCTTTTACCGGCAACAACTCActct > 1:7444642/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGCGGC‑TCCCCCGCCCGCACC‑AAATTTCTCTTTAACGGACAAAACCTCCctct > 2:4734761/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCActct < 1:574455/76‑1 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGG‑CCCCCTGCCATAACCAAATTTTTTTTTTAAC‑GACAACACGTTACTCtt > 1:2673606/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCCTAACC‑CAATTTTTCTTTTTCCGACAACACCTTACCCtt > 2:6043434/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGC‑TCCCCTGCCATAACC‑AATTTTTTCTTTTACGGACAAAAATTCACTCtt > 1:5886681/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCtt < 1:654903/76‑1 (MQ=255) aTCGAAAATAATGTGGCTGGGGGGGTC‑CCCCCTGCCATAAAC‑AATTTTCTTTTTTACGGGCAAAACTTCACTCTTc > 2:6576740/1‑76 (MQ=255) aTCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTc < 2:8850142/76‑1 (MQ=255) tCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCa < 2:508809/76‑1 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCAt < 1:4334960/76‑1 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCAt < 1:1927076/76‑1 (MQ=255) ataatGTGGCTGGGGGGGGC‑TCCCCTCCCATAACC‑AATTTTTTTTTTTACGGAAAACACTTTCCtcttcttcttct > 2:2832504/1‑76 (MQ=255) taatGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtt < 2:3380585/76‑1 (MQ=255) taatGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtt < 1:4574259/76‑1 (MQ=255) aaggtgGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCttt < 1:1078579/73‑1 (MQ=255) atGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt > 1:90248/1‑76 (MQ=255) atGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:2571847/76‑1 (MQ=255) gtgGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTcc < 2:2201467/76‑1 (MQ=255) tggctgggGGGGCC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCCTCTGCTTTTCCt > 2:55983/1‑76 (MQ=255) tggctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt < 2:1544187/76‑1 (MQ=255) tggctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt > 2:8460855/1‑76 (MQ=255) ggctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt < 1:7635691/76‑1 (MQ=255) ggctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt < 1:1233408/76‑1 (MQ=255) gctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTg < 1:6670854/76‑1 (MQ=255) ctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 2:7810505/1‑76 (MQ=255) ctggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCAGCTTTTCCTTGa > 2:5969258/1‑76 (MQ=255) tggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGAt < 2:3450422/76‑1 (MQ=255) tggcGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGAt > 1:4724927/1‑76 (MQ=255) | CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTC‑TCCCATGCCATAACC‑AATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |