| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,180,249 | 0 | T | G | 40.8% | 227.4 / 55.7 | 147 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (33/54); new base G (60/0); total (93/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-17 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 268 total aligned reads displayed.
GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 | ggCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGt < 2:3478602/76‑1 (MQ=255) gCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTg < 2:93920/76‑1 (MQ=255) aGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTgcg < 1:7762693/76‑1 (MQ=255) tGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCt > 2:140652/1‑76 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTg > 2:6632955/1‑76 (MQ=255) cAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGgtt > 1:2675188/1‑76 (MQ=255) aGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtc < 1:6983410/76‑1 (MQ=255) ttttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTtttc > 2:4834428/1‑76 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCa < 1:8145209/76‑1 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGGTGGTtttccc > 1:3314791/1‑74 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTTACt > 2:3209667/1‑76 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACt > 2:1716636/1‑76 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTg < 1:8081539/76‑1 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTctctc > 1:148600/1‑75 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCCGTTTTTTACTAcc > 2:3594685/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCt < 2:5183991/76‑1 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCt < 2:4081618/76‑1 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTTccc > 2:2731669/1‑75 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTg < 1:1043010/76‑1 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGt < 2:7906228/76‑1 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTTACTGcccgg > 2:6188779/1‑73 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTGCCCGt > 1:3932082/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCACTGCCTGTa > 1:6467894/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 2:2153954/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 1:4435648/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 2:7455454/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTa > 2:1330804/1‑76 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:5843920/76‑1 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCca < 2:5060413/76‑1 (MQ=255) ggtTTCCCCCCTTTTGTTTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTTTAGCcac < 2:3913831/76‑1 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCCGTAGcccc > 2:860756/1‑74 (MQ=255) gtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGGTTCTTACCGCCTGTAGCcaca > 2:8467018/1‑76 (MQ=255) gtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTCACTGCCTGTCGCcaca > 1:3473667/1‑76 (MQ=255) tttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTTCCTCTAGCCACaa > 2:2697878/1‑76 (MQ=255) ttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTGCCTGTAGGCACAAc > 1:5027825/1‑76 (MQ=255) tccccccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAAct < 1:659493/73‑1 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGGTTCTTCCTGCCTTTAGCCACAAct > 1:6325102/1‑76 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTTTCACTGCCTGTAGCCACaaac > 1:670598/1‑74 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTTCATGCCTGTAGCCCCAAct‑c > 1:7192437/1‑76 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCAGTTTTTCACTGCCTGCAGCCCCAAct‑ct > 2:8239837/1‑76 (MQ=255) ctttttGTCTTTCTGGCGTCGATCATTGATGCTGGCGGGTGCGCTTTTTCTCACTGCCTTTAGCCACAACT‑CTTCa < 2:8917711/74‑1 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCa < 1:6191286/76‑1 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCa > 1:2011513/1‑76 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCACTGCCTGTAGCCACAACT‑CTTccc > 2:5431848/1‑74 (MQ=255) ttttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCCCTGCCTGTAGCCACAACT‑CTTCACt < 1:1332859/76‑1 (MQ=255) ttttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTTCCTGTAGCCCCCACC‑CCTCACt > 1:2275759/1‑76 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACT‑CTTCACtt > 1:414961/1‑76 (MQ=255) tGTTTTTCGGGCGTCGATCATTGATGCTGGCGGGCGCGCTGTTCCTCCCTGCTTGTAGCCACAACT‑CTTCACTtcc < 2:5260221/76‑1 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCACTGCCTGTAGCCACCACC‑CTTCACTtcc > 2:4660130/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTtcc > 1:850568/1‑76 (MQ=255) ttCTTTCGGGCGTCGATCATTGATGCTGGCAGGTGCGCTTTTTCCCCCTGCCTTTAGCCACAACT‑CTTCACTtcct < 1:984997/75‑1 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTTCCTGTAGCCCCAACA‑CTTCACTtcccc > 1:4242584/1‑74 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTCACCACAACT‑CTTCACTtcctc > 1:2093676/1‑76 (MQ=255) tCTTTCTGGCGTCGACCAGTGATGCTGGCAGGTGCGTTGTTTCTCCCTGCCTGTAGCCACAACT‑CTTCACTtcctc < 1:3157155/76‑1 (MQ=255) ttttCGGGGGTCGTTAATGGATGTGGGCGGGTGCGCTTTTTCTCCCTGCTTTTAGCCACAACT‑CTTCACTtcctcc < 2:3349232/75‑1 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTGGCCGTAGCCACAACT‑CTTCACTttcttc > 1:5176316/1‑74 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGACACAAAT‑CTTCACCtcctcc > 2:2507802/1‑76 (MQ=255) cTTTCTGGCGTAGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAAAT‑CTTCACTtcctcc > 2:320485/1‑76 (MQ=255) tttCGGGCGTCGATCATTGATGCGGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTccc < 1:3019903/76‑1 (MQ=255) ttgtGGCGTCTTTCTTTGATGCTGGCAGGTGCGTTTTTTCTCACTCCCTTTAGCCACAACT‑CTTCACTTCCTCCCt < 2:4270489/73‑1 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCt > 1:5988687/1‑76 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACCGCCTGTAGCCACAACT‑CTTTACTTCCTCCCtt > 2:5875941/1‑76 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCCGTAGCCACAACT‑CTTCACTTCCTCCCtt > 2:6149089/1‑76 (MQ=255) cTGGCGTGGATTTTTGATGCTGGCGGGTGCTCTTTTTCTCCCTTCCTGTAGCCACAACT‑CTTCACTTCCTCCCttt < 2:7288994/76‑1 (MQ=255) cTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTTGCCACAACT‑CTTCACTCTCACCCttt > 2:4221268/1‑76 (MQ=255) cTGGCGTCGATCATTGATGCTGGCAGGGGGGCGGTTTTTCACTGCCTGTAGCCACCACT‑CTTCACTTCCTCCCctt > 1:7610387/1‑76 (MQ=255) tGGCGTCGATCATTGATGCTGGCAGGTGGGCTGTTTCTTACTGCCTGTTGGCAAAACT‑CTTCACCTCCTCCCtttt > 1:7403111/1‑75 (MQ=255) tGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCcttt > 2:7329269/1‑75 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTTACTGCCTGTAGCCCCAAATCCTCCACTT‑CTCCCttttc > 1:6776311/1‑74 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGGCACAAAT‑CTTCACTTCCTCCCTTTAc > 2:771558/1‑76 (MQ=255) gCGTGGATCTTTAAGCCTGGCGGGTCCCTTTTTTCCCCCTCCTTGTAGCCACAACT‑CTTCACTTCCTCCCTTTAcc < 1:7370165/76‑1 (MQ=255) gCGTCGATCTTTGCTGCTGGGAGGTGCGTTGTTTCCCCCTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTAcc < 1:1853130/76‑1 (MQ=255) cGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTGCCCGTAGCCACAAAT‑CTTCACTTCCTCCCTTTTccc > 2:7961844/1‑75 (MQ=255) gTCGATTTTTGATGCTGGCGGGGGCGCGTTTTCCCCCTGCTTTTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGc < 2:7640782/76‑1 (MQ=255) gTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCCGTAGGCACAACT‑CTTCACTTTCTCCCTTTACCGc > 1:1806155/1‑76 (MQ=255) cGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCa > 2:3091773/1‑76 (MQ=255) cGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGccc > 1:4677411/1‑75 (MQ=255) gATCATTGATGCTGGCAGGGGCGCTGTTTTTCACCGCCTGTAGCCACCAAC‑CTTCACTTTCTCCCCTTTCCCCCac > 2:594703/1‑75 (MQ=255) aTTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGa > 1:4823569/1‑76 (MQ=255) tGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTTCTCCCTTTACCGCCCGTGGAtt > 1:7973826/1‑76 (MQ=255) tGATGCTGGCAGGGGCGCGGTTTTTCACTTCCCGTGGCGCCCACA‑CTTCCACTCCTCCCCTTACCCCCCGCGGAtt > 2:4706375/1‑76 (MQ=255) cTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGccg > 2:4018688/1‑74 (MQ=255) cTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTg > 1:6029128/1‑76 (MQ=255) cTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTg > 1:7460086/1‑76 (MQ=255) cTGGCAGGGGCGCTGTTTCTCACTGCCTATAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTg > 2:1544583/1‑76 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGGCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGCGGATTTGCTGa > 2:2016161/1‑76 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 2:8421196/1‑76 (MQ=255) tGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 2:2925360/1‑76 (MQ=255) ggCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGaa > 2:2298007/1‑76 (MQ=255) ggCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGaa > 2:7174762/1‑76 (MQ=255) ggCAGGGGCGCCTTTTCGCACTGCTTGTAGCTCCGACT‑CTTCACTTCCTCCTCTTACGCCCAGTGGACTTGCTgca > 2:4857252/1‑74 (MQ=255) gCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAg > 2:4555301/1‑76 (MQ=255) gCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAg > 1:7052992/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:3838301/1‑76 (MQ=255) ggTGCGTTTTTCCTCCCGGCCTTTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAcc < 1:8810470/76‑1 (MQ=255) gTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCa > 1:1426217/1‑76 (MQ=255) tGCTCTGTTTCCCCCCCCTTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg < 1:1518438/76‑1 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:8870246/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:8374273/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:5439938/1‑76 (MQ=255) | GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACT‑CTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |