| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 35.9% | 245.1 / 113.0 | 181 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (77/39); new base T (0/65); total (77/104) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 282 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 1:5394177/76‑1 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 2:4178515/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:6110313/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa > 2:548644/1‑76 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 1:5601635/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 1:4209837/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGcc > 1:372208/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:6683291/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 2:1665482/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 2:244550/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 1:8152920/76‑1 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTACCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 2:7449271/76‑1 (MQ=255) tataCCTGTCGGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 2:7155877/76‑1 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 2:8774380/1‑76 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAgca > 2:5414065/1‑74 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTTGCCAGTAt > 1:1143832/1‑76 (MQ=255) ccTGTTGGATGGCGCGCTAGGGGAAGGGGTACCGGGTGAGGGTGCTGGCTGGGATGGCTTTTTTTTAGCCAGTAtt < 2:2732318/76‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTTGGGCTTTTCTAACCAGTAtt > 1:6065454/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGTTTTTCTAGCCAGTATTg > 1:2084459/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGGGATTTTCTAGCCAGTATTg > 1:7454763/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGGCAGTATTg > 2:522087/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 2:6232230/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCAGTAACGCGTGAAGGTGCTGGCTGGGGTAGAGGTTTTCTAGCCCGTATTg > 1:768291/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTggg > 1:2279571/1‑75 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCTGTTTTCTACCCAGGATTgggt > 1:1347457/1‑74 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTc > 2:8217244/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTc < 2:4125376/76‑1 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGGca > 1:3851428/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGTATTTGGcag > 1:4137846/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTTGTtagc > 2:7964542/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcagc > 2:2201387/1‑76 (MQ=255) agggCGCGCGCTGCGAAGCGGTAACGCGTGAAGGGGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcagca < 2:5137825/74‑1 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGGGCTGGCTGGGGTAGCGGTTTTCTAGCCCGAATTGGTccgcc > 1:984810/1‑75 (MQ=255) tGGCGCGCTCTGCAAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTcagcag < 1:6828720/76‑1 (MQ=255) ggCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGt < 1:4454201/76‑1 (MQ=255) gcgcgcTCTGCGAGGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGtt < 1:3307429/76‑1 (MQ=255) ggcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTa < 1:1073115/75‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGGGTGAGGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAg < 1:3634674/76‑1 (MQ=255) cgcTCTGCGAAGCGGTACCGGGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 2:4835179/76‑1 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGGCAGCCGTTAgg > 1:5777864/1‑76 (MQ=255) cgcTCTGCCAAGCGCGAACGCGTGAAGGTGCTGGCCGGGAAAGCCTTTTTCTAGCCAGTATTGGTCAGCAGTTAgg < 1:8599006/76‑1 (MQ=255) cgcTCGGCGAAGCGGTAACGGGTGAGGGTGCTGGCGGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg < 1:6536429/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGGAGGTAGGa > 1:5285192/1‑76 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTTGTCACCAGTTCggga > 2:920297/1‑74 (MQ=255) ctcgGCGAAGCGTTAACGCGTGAAGGTGCTGGGTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:2730496/76‑1 (MQ=255) ctcgGCGAAGCGGTAACGCGTGAAGGTGCGGGCTGGGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:1608639/76‑1 (MQ=255) ctGGGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATACGGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 1:6618329/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg < 2:6608401/76‑1 (MQ=255) cggCGAGGCGTTAACGCGTGAGGGTGCTGGCGGGAATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg < 1:3785998/74‑1 (MQ=255) tGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGGCAGCAGTTAGGAACgg > 1:5152498/1‑76 (MQ=255) tGCGAAGCGGTAACGCGTGAAGGTGCTGGCCGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgg > 1:2926533/1‑76 (MQ=255) aggCGGTAAGGCGGGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:8279568/74‑1 (MQ=255) aaGGGGTAACGCGTGAAGGTGCTGGCGGGGAGAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:2569889/76‑1 (MQ=255) aaGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCCGTTTGGAAAGGCTGa > 1:4735218/1‑76 (MQ=255) aaGCGGAAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:8749578/76‑1 (MQ=255) gggTAACGCTTGAGGGTGCTGGTTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATc < 1:2487659/75‑1 (MQ=255) cGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAACCAGTCTTGGGCAACAGTTAAGAACGGCTGaaat > 2:6863607/1‑74 (MQ=255) tAACGGGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt < 2:6404882/76‑1 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt > 2:144734/1‑76 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt > 1:7263674/1‑76 (MQ=255) aCGCGTGAAGGTGCTGGGGGGGATGGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTg < 1:8311366/76‑1 (MQ=255) ggggTGAAGGTGCGGGCGGGGATAGCTATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 1:4172521/73‑1 (MQ=255) gggTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:7329017/74‑1 (MQ=255) gtaaaGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt < 2:1185871/73‑1 (MQ=255) gggAAGGTGCTGGCGGGGATAGCTATTTTTTAGCCAGTACTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt < 1:2450533/74‑1 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAATg > 2:4571929/1‑76 (MQ=255) agggTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg < 1:6970275/74‑1 (MQ=255) aGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg > 1:222168/1‑76 (MQ=255) gTGCTGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 1:1551964/76‑1 (MQ=255) tGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgg > 1:8189880/1‑76 (MQ=255) gtggctggGATAGCGTTTTTTTAGCCAGGATGGGTCAGCAGTTAGGAACGGCTGAAGCCCTGGAACTGGGGCGGCt < 2:7771972/75‑1 (MQ=255) tggctggGTTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTTAACTGGGGCGGCTg > 2:6714364/1‑76 (MQ=255) tggctggGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 2:5936476/76‑1 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg > 2:1829824/1‑76 (MQ=255) ggctggGATAGGGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:2596679/76‑1 (MQ=255) gcggggATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 1:1250050/73‑1 (MQ=255) gtggGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg < 1:8523988/75‑1 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg > 1:1745368/1‑76 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg > 1:7526403/1‑76 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggca > 2:8090219/1‑73 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGcca > 1:7797188/1‑74 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCgg > 1:3514971/1‑75 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGa > 1:735780/1‑76 (MQ=255) gggATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGaa < 1:8763496/76‑1 (MQ=255) gggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCgga > 1:1016673/1‑74 (MQ=255) ggATAGTGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAt < 1:848234/76‑1 (MQ=255) aTAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 2:2828277/76‑1 (MQ=255) aTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGAtgg > 1:5840325/1‑75 (MQ=255) tAGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgtg < 2:4007149/76‑1 (MQ=255) tAGCGATTTTCTAGCCAGTATTGGTCAGCCGTTAGGTACGGCTGAATTCCTTGTACTGGGGCGGCTGGGGAAttag > 2:3097087/1‑73 (MQ=255) aGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa > 2:3400350/1‑53 (MQ=255) aGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTTGCGGAAGTGa > 2:6068820/1‑76 (MQ=255) cttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:4844623/73‑1 (MQ=255) gtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 1:6313889/74‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 2:3779179/75‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 1:8690069/75‑1 (MQ=255) ttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCg < 1:144734/76‑1 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGCATGGTAATCCGc > 1:3652859/1‑76 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGc > 1:6192727/1‑76 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGAGAATcccc > 1:4325426/1‑74 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |