| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,444 | A→G | S288P (TCC→CCC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,444 | 0 | A | G | 99.6% | 1009.9 / NA | 242 | S288P (TCC→CCC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (139/102); minor base T (1/0); total (140/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 259 total aligned reads displayed.
ATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGA > REL606/474371‑474516 | ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga > 1:6136137/1‑76 (MQ=255)ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga < 1:1343504/76‑1 (MQ=255)ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga > 1:7553880/1‑76 (MQ=255)ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga > 2:5411781/1‑76 (MQ=255)ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga > 1:8581837/1‑76 (MQ=255)ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGggga > 1:3876699/1‑76 (MQ=255) taatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGAc < 2:6672475/76‑1 (MQ=255) agtCGCCGGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACa < 2:163311/74‑1 (MQ=255) aatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACa > 2:2272953/1‑76 (MQ=255) atCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACaa > 1:3177646/1‑76 (MQ=255) tCGCCGGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACaaa < 2:4954598/76‑1 (MQ=255) tCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACaaa > 2:1924915/1‑76 (MQ=255) tCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACaaa > 2:4720476/1‑76 (MQ=255) tCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACaaa < 1:4126796/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAAc < 2:1290953/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAAc > 1:8507330/1‑76 (MQ=255) aGCGTCGCCAGCACTGCCCGCGGTTGCTGAATCAGAATTACCGAATCAAACAACATGCCGACGGGGACAAAAAACa > 2:4282262/1‑76 (MQ=255) aGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACa > 2:7805014/1‑76 (MQ=255) gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCa > 1:2198872/1‑76 (MQ=255) gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCa < 1:8473832/76‑1 (MQ=255) gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCa > 2:6910458/1‑76 (MQ=255) cGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCAcc > 2:4473990/1‑76 (MQ=255) cGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCAcc > 1:4380681/1‑76 (MQ=255) gcaaGCCCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCg < 1:2628605/73‑1 (MQ=255) gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAACAACAGCACCg > 1:2559503/1‑76 (MQ=255) gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCg < 2:8803572/76‑1 (MQ=255) gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCg > 2:4768877/1‑76 (MQ=255) gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCg < 1:232646/76‑1 (MQ=255) gCCAGCACTCCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCg < 2:3438443/76‑1 (MQ=255) ccAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGc > 2:750438/1‑76 (MQ=255) cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCa > 1:3954482/1‑76 (MQ=255) cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCa > 2:8728198/1‑76 (MQ=255) cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCa < 2:7868081/76‑1 (MQ=255) gtcACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCaa < 1:3746368/74‑1 (MQ=255) cACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAAc < 2:6329654/76‑1 (MQ=255) ccTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAAcg < 1:8793956/75‑1 (MQ=255) tGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCCCCGCAAAcgcg < 2:5740191/76‑1 (MQ=255) gCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGt < 2:5297400/76‑1 (MQ=255) ccAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTc < 2:2506489/76‑1 (MQ=255) cAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTcg < 1:6738384/76‑1 (MQ=255) cAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTcg < 1:7649148/76‑1 (MQ=255) gCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTcgcg > 1:7393413/1‑76 (MQ=255) cGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCgcgc < 1:8376443/76‑1 (MQ=255) ggTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCa > 1:5480462/1‑76 (MQ=255) gTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCaa > 1:5392403/1‑76 (MQ=255) ttGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAAt > 1:1598297/1‑76 (MQ=255) ttGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAAt > 1:1361297/1‑76 (MQ=255) tGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg < 1:3019012/76‑1 (MQ=255) tGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg > 1:7997245/1‑76 (MQ=255) tGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg > 1:3447363/1‑76 (MQ=255) gCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATgg > 2:3894307/1‑76 (MQ=255) gCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATgg > 2:3055510/1‑76 (MQ=255) cTGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGc > 1:1185855/1‑76 (MQ=255) tGAATCAGAATTAACGGATCAAACAACATCCCGCCGGTGACAAAAAACCGAACCGCAAACGCGTCGCGCAGTGGCa > 2:7135730/1‑76 (MQ=255) tGAATCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCa < 2:6631585/76‑1 (MQ=255) aaTCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGc > 1:4196580/1‑76 (MQ=255) aTCGGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCg < 2:2586266/76‑1 (MQ=255) aTCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCg > 2:5545300/1‑76 (MQ=255) aTCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCg > 1:6558763/1‑76 (MQ=255) tCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGt < 1:1960019/76‑1 (MQ=255) tCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGt < 1:8762277/76‑1 (MQ=255) tCAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGt < 1:8205544/76‑1 (MQ=255) cAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa < 1:4928119/76‑1 (MQ=255) cAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa > 1:6356254/1‑76 (MQ=255) cAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa > 1:1526505/1‑76 (MQ=255) cAGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa > 1:5623301/1‑76 (MQ=255) aGAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt > 1:4061003/1‑76 (MQ=255) gAATTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATc < 1:3302978/76‑1 (MQ=255) aaTTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCg < 1:2997996/76‑1 (MQ=255) aaTTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCg > 2:6514132/1‑76 (MQ=255) aTTAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGt < 2:1979869/76‑1 (MQ=255) ttAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg > 2:7670264/1‑76 (MQ=255) tAACGGATCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg > 1:1123453/1‑76 (MQ=255) aaCGGATCAAACAACATCCCGACGGGTACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTggg < 2:4193753/76‑1 (MQ=255) aTCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCa < 2:2147108/76‑1 (MQ=255) aTCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCa < 2:3093062/76‑1 (MQ=255) tCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggcggccc > 2:8112070/1‑74 (MQ=255) tCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc > 1:5718331/1‑76 (MQ=255) tCAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc > 1:8333629/1‑76 (MQ=255) acAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc < 1:4782745/75‑1 (MQ=255) cAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACg > 2:8021649/1‑76 (MQ=255) cAAACAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGGTTCGTGgggggcctg > 1:7063793/1‑73 (MQ=255) aaaCACCAACCCGACGGGGACAAAAAACAGCACCTCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACgg < 2:8595859/76‑1 (MQ=255) aaaCAACATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACgg > 2:6118313/1‑76 (MQ=255) aacaacATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGt < 2:3510778/76‑1 (MQ=255) aacaacATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGt < 2:7279949/76‑1 (MQ=255) aacaacATCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGt < 1:1877914/76‑1 (MQ=255) acaacaTCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg < 1:7821235/76‑1 (MQ=255) acaacaTCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg > 1:767001/1‑76 (MQ=255) acaacaTCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg < 2:1366968/76‑1 (MQ=255) caacaTCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGGGa > 2:739090/1‑76 (MQ=255) acaTCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGCGGGCGGCCCGGGGACt > 2:2886161/1‑76 (MQ=255) tCCCGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAg < 2:5067933/76‑1 (MQ=255) cccGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGACCGGGGACTGAGt > 1:6007430/1‑76 (MQ=255) ccGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGtt < 1:5265349/76‑1 (MQ=255) ccGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGtt > 2:4642424/1‑76 (MQ=255) cGACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGGGGGCGGCAGGGTGAGTCAGTTc > 1:5085890/1‑76 (MQ=255) gACGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCa < 2:4373540/76‑1 (MQ=255) aCGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAg < 2:5914675/76‑1 (MQ=255) aCGGGGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAg > 1:6650242/1‑76 (MQ=255) | ATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGA > REL606/474371‑474516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |