| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,161,969 | C→T | P372S (CCT→TCT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,161,969 | 0 | C | T | 98.5% | 760.8 / ‑0.9 | 196 | P372S (CCT→TCT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (157/36); total (158/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
Only 100 of 290 total aligned reads displayed.
CTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161896‑4162041 | ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct < 2:7495637/76‑1 (MQ=255)ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct > 1:1487448/1‑76 (MQ=255)ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct < 2:6769286/76‑1 (MQ=255)ctctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGctct > 1:5123456/1‑76 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:2119229/76‑1 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 1:8393618/76‑1 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:8306102/76‑1 (MQ=255) tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTg < 2:5370964/76‑1 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:4365176/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:409712/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg < 2:8369610/76‑1 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 1:7969618/1‑76 (MQ=255) ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg > 2:2864676/1‑76 (MQ=255) gAAACCTTACGTGTCGGCCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg < 1:3342967/76‑1 (MQ=255) gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg > 2:5951864/1‑76 (MQ=255) gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCg < 2:7422903/76‑1 (MQ=255) gAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCAGGCg < 2:432344/76‑1 (MQ=255) aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGa < 1:7006206/76‑1 (MQ=255) aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGa > 2:4407586/1‑76 (MQ=255) aCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAgc > 1:1081253/1‑76 (MQ=255) cTTCCGGGTCGCCCAAACTAACGCCCGTCGGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCTCGGGGGAgccg < 2:4624239/76‑1 (MQ=255) cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccg > 2:1469719/1‑76 (MQ=255) cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGAGGCGCTCTGGCGAgcca < 2:4212130/76‑2 (MQ=255) ttACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgc > 1:2526119/1‑76 (MQ=255) tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgccgcc > 1:2899108/1‑76 (MQ=255) cGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGa > 2:7613247/1‑76 (MQ=255) cGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCAGa > 2:2705907/1‑76 (MQ=255) tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACt > 2:6608699/1‑76 (MQ=255) gtCGACCCAACTAACGACCGTCTGGGCGCACTGGTAAAAATCTACCGCATGATGCGCTCTGGGGAGCCGCCGACTc < 2:865412/76‑1 (MQ=255) gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTc > 1:56398/1‑76 (MQ=255) gccGACACAACTAACGACCGTCTGAGCGCACGGTTAGAAACCTACCGCATGTTCCGCTCGGGGGAGCCGCCGACTc < 2:6566645/74‑1 (MQ=255) gACCCAACTACCGACCGTCGGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTg < 2:4845578/76‑1 (MQ=255) gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:5690041/1‑76 (MQ=255) gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTg > 2:616046/1‑76 (MQ=255) caCCCAATTAAGGACCGCCTGAGCCCGAGGTAAAAAATCTCCGGCATAATGCGCGCGGGGGAGCCGCCGACTCGTg < 2:235153/75‑1 (MQ=255) cccAACTACCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGaa < 1:1606716/76‑1 (MQ=255) ccACCTAACGACGGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAag < 2:7860487/76‑1 (MQ=255) ccAACTAACGACCGTCTGGGCGCCCTGGTAAAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAag < 2:3128273/76‑1 (MQ=255) cAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc > 1:6512640/1‑76 (MQ=255) cAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc > 1:1247384/1‑76 (MQ=255) aaCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagca > 2:1619000/1‑76 (MQ=255) aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcag > 1:7678235/1‑76 (MQ=255) aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCACTCTGGCGAGCCGCCGACTCGTGAagcag > 1:211170/1‑76 (MQ=255) cTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagcagc > 2:6175614/1‑76 (MQ=255) aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 1:2639948/1‑76 (MQ=255) cGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa > 1:7258838/1‑76 (MQ=255) gcccGTCTGAGCGCCCTGGTAGAAATCTACCGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 1:5231508/74‑1 (MQ=255) gcccGTCTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 1:7829139/74‑1 (MQ=255) gACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:3091259/1‑76 (MQ=255) gACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 2:1563644/1‑76 (MQ=255) cccGTCTGAGCGCCCTGGTAGAAATCTACCGCATGATGCGTTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:2348395/75‑1 (MQ=255) cccGTCTGAGCGCATGGGTAGAAATCTCCCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 2:3466271/75‑1 (MQ=255) ccGTCTGAGCGCCGTGGTAGAAATCTCCCGCATGATGCGCGCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGc < 2:6015129/76‑1 (MQ=255) cGTCTGCGCGCGTGGGAAGAAATCTCCGGCATGATGCCCTCTGGCGGGCCGCCGACTCGTGAAGCAGCGGAAAGcc < 1:4811943/76‑1 (MQ=255) cGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGcc > 1:4191984/1‑76 (MQ=255) gTCTGAGCGCCCTGTTAGAAACCTCCCGCATGATGCGCGAGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:1199202/76‑1 (MQ=255) gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt > 2:883991/1‑76 (MQ=255) gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:4362766/76‑1 (MQ=255) tCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTg > 1:7376714/1‑76 (MQ=255) cttaGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt > 2:1233967/4‑76 (MQ=255) cggAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt < 1:7235263/74‑1 (MQ=255) tGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:4945144/76‑1 (MQ=255) tGAGCGCACTGGTAAAATTCTCCCGAATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:3697682/76‑1 (MQ=255) ggAGCCCCCTGGAAGAAATCTACCGCATGATGCGCTTGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 2:3913649/75‑1 (MQ=255) gAGCGCACTGGTAAAAATCTACCGCAGGAGGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc < 2:2561688/76‑1 (MQ=255) aGCGCCCGGGTAGAAATCTCCCGCATGAGGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCg < 1:7344929/76‑1 (MQ=255) cgcACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag > 2:564410/1‑76 (MQ=255) cgcACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag > 1:1808161/1‑76 (MQ=255) gcACGGGTAGAAATCTCCCGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgaga < 1:453785/76‑1 (MQ=255) ccTGGAAAAAACCTCCCGCATGAGGCGCTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAc < 2:8430222/75‑1 (MQ=255) aCTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAc > 2:1993780/1‑76 (MQ=255) cTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 1:8319469/1‑76 (MQ=255) aGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttc > 1:4605819/1‑76 (MQ=255) aGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttc > 1:951139/1‑76 (MQ=255) atttctcCCACATGATGCGCTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 2:1755571/73‑1 (MQ=255) aaaTCTCCCGCGTGAGGCGTCTGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:5954464/76‑1 (MQ=255) aaaTCTACCTCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 2:2235395/76‑1 (MQ=255) aaaTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt > 1:673652/1‑76 (MQ=255) aaaTCTACCCCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:6469420/76‑1 (MQ=255) tCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGACGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTtctc > 1:5322092/1‑76 (MQ=255) cTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTcc > 1:7520039/1‑76 (MQ=255) tACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg > 2:691119/1‑76 (MQ=255) aCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGa > 1:8618722/1‑76 (MQ=255) aCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGa > 1:6797428/1‑76 (MQ=255) ccGCATGATGCGCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGaa < 2:802356/76‑1 (MQ=255) ccGAATAATGCCCTCGGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGaa < 2:3824755/76‑1 (MQ=255) cGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg < 1:8871808/76‑1 (MQ=255) cGCATGAGGCCCTGTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg < 2:2457069/76‑1 (MQ=255) cATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc > 1:295645/1‑76 (MQ=255) cATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc > 2:5082482/1‑76 (MQ=255) cATGAGGCCCTCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAc < 1:3394089/76‑1 (MQ=255) atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 2:6393136/1‑76 (MQ=255) atgatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc > 1:8458615/1‑76 (MQ=255) gatgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGt > 1:868358/1‑76 (MQ=255) atgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt < 1:3872000/76‑1 (MQ=255) atgCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt > 2:5191574/1‑76 (MQ=255) agccgcTCTGGGGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt < 1:5688705/73‑1 (MQ=255) tgCGCTCTGGCGAGCCGCCGACTCGTAAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTa > 2:7283092/1‑76 (MQ=255) gcgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTAt < 1:102205/76‑1 (MQ=255) cgcTCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATg > 2:3217162/1‑76 (MQ=255) | CTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATG > REL606/4161896‑4162041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |