| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,079,196 | C→T | L290L (CTG→CTA) | cbpA ← | curved DNA‑binding protein, DnaJ homologue that functions as a co‑chaperone of DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,079,196 | 0 | C | T | 99.0% | 808.9 / ‑3.0 | 209 | L290L (CTG→CTA) | cbpA | curved DNA‑binding protein, DnaJ homologue that functions as a co‑chaperone of DnaK |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (110/97); total (111/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 220 total aligned reads displayed.
AAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGA > REL606/1079123‑1079268 | aaaGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAg > 1:1665984/1‑76 (MQ=255)aaaGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAg < 2:5849125/76‑1 (MQ=255) aaGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGt > 2:4557392/1‑76 (MQ=255) gTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtg < 2:7602364/76‑1 (MQ=255) tCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtgc > 2:7346258/1‑76 (MQ=255) tCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtgc > 2:4389598/1‑76 (MQ=255) aCCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtgctg > 1:4554282/1‑76 (MQ=255) ccGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtgctgc < 1:6339418/76‑1 (MQ=255) ccGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTtgctgc < 1:1434253/76‑1 (MQ=255) gTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCca > 1:6701187/1‑76 (MQ=255) tAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCcac < 1:1933918/76‑1 (MQ=255) tAACATTAGCCATTATGCTTCCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCcac < 2:8299093/76‑1 (MQ=255) aaCATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCcaca > 1:6957506/1‑76 (MQ=255) aaCATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCcaca > 2:7741355/1‑76 (MQ=255) aCATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAg < 2:598797/76‑1 (MQ=255) aCATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAg < 2:3877588/76‑1 (MQ=255) cgttAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAgc > 1:2614462/3‑76 (MQ=255) aTTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAgcg < 1:2958914/76‑1 (MQ=255) ttAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAgcgc < 2:2836328/76‑1 (MQ=255) aGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCgg < 2:1681543/76‑1 (MQ=255) aGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCgg < 1:7547686/76‑1 (MQ=255) cATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAg > 2:8175544/1‑76 (MQ=255) cATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAg > 2:302027/1‑76 (MQ=255) aTTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAgg > 2:7847013/1‑75 (MQ=255) aTTATGCTTTCCCCCAATCTTTACGTGGAGCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAgt < 2:1216071/76‑1 (MQ=255) ttATGCTTTCCCCCAATCTTTACGTGAATCAAAAGACGACTGGGCGTCTGGTAGTTGCTGCCACAGCGCGGCAgtg < 2:7919828/76‑1 (MQ=255) tATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAgtgt < 2:3361511/76‑1 (MQ=255) tGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGttt < 1:7953315/76‑1 (MQ=255) tcTTCCCCCCAATCTTTACGGGGAACAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGtttt < 1:3307158/75‑1 (MQ=255) gCTTTCCCCCATTCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCAACAGCGCCGAAGTGtttt > 2:3971205/1‑76 (MQ=255) gCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGtttt < 1:5128985/76‑1 (MQ=255) gCTTTCCCCAAATTTTTACGTGGATCAAAAGACGACGGGGCGTCTGCTATTTGCTGCCACAGCGCGGCAGTGtttt < 2:8422756/76‑1 (MQ=255) cTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTc > 1:1094500/1‑76 (MQ=255) cTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGCGTTTTc > 2:6095239/1‑76 (MQ=255) tttCCCCCAATCTTTCCGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCa < 1:5606305/76‑1 (MQ=255) tttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCa > 2:2541843/1‑76 (MQ=255) tttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCa > 1:1547026/1‑76 (MQ=255) ttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTTCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCAt < 2:786987/76‑1 (MQ=255) ttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCAt > 2:3822183/1‑76 (MQ=255) ttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCAt < 1:6264288/76‑1 (MQ=255) ttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGGTTTCAt > 2:41487/1‑76 (MQ=255) tCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATc > 2:4729418/1‑76 (MQ=255) tCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATc > 2:8007837/1‑76 (MQ=255) cccccAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATcc > 1:880627/1‑76 (MQ=255) cccccAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGGGTTTTCATcc > 1:4847882/1‑76 (MQ=255) ccccAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCg > 2:8126058/1‑76 (MQ=255) cccAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATTCgg > 1:5701556/1‑76 (MQ=255) cAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGtt < 2:7025839/76‑1 (MQ=255) aaTCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGttt < 2:5286599/76‑1 (MQ=255) aaTCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGttt < 1:8383142/76‑1 (MQ=255) aTCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTc > 2:2148631/1‑76 (MQ=255) cTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcgg > 2:3173065/1‑76 (MQ=255) cTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcgg > 2:1334820/1‑76 (MQ=255) cTTTACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcgg > 1:2938516/1‑76 (MQ=255) tttACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc > 1:3079614/1‑76 (MQ=255) tttACGGGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc < 1:4151131/76‑1 (MQ=255) ttACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggcg > 1:1853015/1‑76 (MQ=255) ttACGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggcg > 1:8176945/1‑76 (MQ=255) aCGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCggcggc < 2:5537630/76‑1 (MQ=255) aCGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCggcggc > 1:545074/1‑76 (MQ=255) cGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCggcggcc > 2:4772835/1‑75 (MQ=255) cGTGGATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCa > 2:1826586/1‑76 (MQ=255) gggATCAAAAGACGCCTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATc < 2:2434165/75‑1 (MQ=255) gggATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATc < 1:5955880/75‑1 (MQ=255) gATCAAAAGGCGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCAc < 1:4019674/76‑1 (MQ=255) gATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCAc < 2:311568/76‑1 (MQ=255) gATCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCAc > 1:30026/1‑76 (MQ=255) tCAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGGCTCACGa > 2:4853306/1‑76 (MQ=255) cAAAAGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAt < 1:987729/76‑1 (MQ=255) aaagGACGCCCGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAtt < 2:4266779/76‑1 (MQ=255) aaaaGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAtt > 2:3703326/1‑76 (MQ=255) aaaGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAttt > 1:2025377/1‑76 (MQ=255) aaaGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCCGCGGCATCACGAttt > 2:7538712/1‑76 (MQ=255) aaGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAtttt < 1:4287786/76‑1 (MQ=255) aGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTc < 1:4400404/76‑1 (MQ=255) aGACGACTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTc > 1:6178378/1‑76 (MQ=255) cgacTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCCGt > 2:2279484/1‑76 (MQ=255) cgacTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGt < 2:1286486/76‑1 (MQ=255) cgacTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGt < 2:659736/76‑1 (MQ=255) cgacTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGt < 1:2633210/76‑1 (MQ=255) acTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCCTCACGATTTTCAGTAc > 2:1770159/1‑76 (MQ=255) acTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTAc > 1:2747211/1‑76 (MQ=255) cTGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCCTCACGATTTTCAGTAcc > 2:6647836/1‑76 (MQ=255) cTGGGCGTCTGCTAGGTGCTGCCACAGCGCGGCAGTGATTTCATCCGGGTTCGGCGGCATCACGATTTTCAGTAcc > 2:6405361/1‑76 (MQ=255) cTGGGCGTCTGCCAGTCGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTAca < 2:2342968/76‑2 (MQ=255) tGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCg < 1:6836858/76‑1 (MQ=255) tGGGCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGGACCg > 1:489645/1‑76 (MQ=255) tGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCg > 2:1606817/1‑76 (MQ=255) ggggCTTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCg < 2:8859737/75‑1 (MQ=255) gCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCAt < 2:6696289/76‑1 (MQ=255) gCGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCAt < 2:4343377/76‑1 (MQ=255) cGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCCTCACGATTTTCAGTACCCCATa > 2:6364635/1‑76 (MQ=255) cGTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATa < 2:2926897/76‑1 (MQ=255) gTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCCTCACGATTTTCCGTAACGCacac > 2:4522403/1‑76 (MQ=255) gTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGGTTTTCCGGACCGCATAc > 2:1461711/1‑76 (MQ=255) gTCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACCATTTTCAGTAACCCATaa > 2:5680595/1‑75 (MQ=255) tCTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACa < 1:1795908/76‑1 (MQ=255) cTGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAg > 1:1199567/1‑76 (MQ=255) tGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGa < 1:2291315/76‑1 (MQ=255) tGCTAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGGTTAGGCCGCATCACGATTTTCAGGACCGCATACAGa > 2:7494495/1‑76 (MQ=255) | AAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGA > REL606/1079123‑1079268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |