| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,164,266 | C→T | D526N (GAT→AAT) | yehU ← | predicted sensory kinase in two‑component system with YehT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,164,266 | 0 | C | T | 99.0% | 1141.5 / 0.0 | 285 | D526N (GAT→AAT) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (148/134); total (150/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
Only 100 of 304 total aligned reads displayed.
CGGGTGTAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATCCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAG > REL606/2164193‑2164338 | ggTGTAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATtcacc < 2:7785984/76‑1 (MQ=255) ggTGTAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATtcacc < 1:6075485/76‑1 (MQ=255) gtgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATtcacca < 2:4208358/76‑1 (MQ=255) gtgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATtcacca < 1:7627731/76‑1 (MQ=255) tgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAg < 1:5475712/76‑1 (MQ=255) tgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAg > 1:5904723/1‑76 (MQ=255) tgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAg > 2:2700575/1‑76 (MQ=255) tgtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAg < 2:14875/76‑1 (MQ=255) gtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGa < 2:3054627/76‑1 (MQ=255) gtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGa > 2:1378466/1‑76 (MQ=255) gtAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGa > 1:8294921/1‑76 (MQ=255) aaCTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGAtt > 2:163121/1‑76 (MQ=255) aaCTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGAtt > 1:8556166/1‑76 (MQ=255) aCTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTc < 1:7036800/76‑1 (MQ=255) cTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCa > 2:7225149/1‑76 (MQ=255) cTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCa < 1:6202079/76‑1 (MQ=255) tATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCAt < 1:931095/76‑1 (MQ=255) tATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCAt > 2:6454582/1‑76 (MQ=255) gtgcAGGCGCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGGTCACGTAAACGCTTTTTCACCAGATTCAt < 2:8693841/73‑1 (MQ=255) aTCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATc > 1:8661607/1‑76 (MQ=255) aTCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATc > 1:1108431/1‑76 (MQ=255) cAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATccc > 2:5858577/1‑76 (MQ=255) cAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTAACGTAAACGCTTATTCACCAGATTCATccc > 1:6733576/1‑76 (MQ=255) ggCTCTCAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCa < 1:5958644/76‑1 (MQ=255) ggCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCCCCAGATTCATCCCCa < 2:7628464/76‑1 (MQ=255) ggCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCa > 1:3407108/1‑76 (MQ=255) ggCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCa < 2:8636179/76‑1 (MQ=255) ggCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCa > 1:6273056/1‑76 (MQ=255) cTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGc > 2:7299982/1‑76 (MQ=255) cTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGc < 2:7439375/76‑1 (MQ=255) cTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGc < 2:1072079/76‑1 (MQ=255) cTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGc > 1:7113582/1‑76 (MQ=255) tCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGcc > 1:410143/1‑76 (MQ=255) cacaGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGccc < 2:8747655/76‑1 (MQ=255) caGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCAc > 2:5484288/1‑76 (MQ=255) aGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACt < 2:212509/76‑1 (MQ=255) aGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACt < 1:5357153/76‑1 (MQ=255) aGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACt > 2:431816/1‑76 (MQ=255) ggCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCGAGCCCACTg < 1:1356795/76‑1 (MQ=255) ggCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTg < 1:7382597/76‑1 (MQ=255) cGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGc < 1:7881474/76‑1 (MQ=255) cGACCCTTATTCCAAAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGc < 1:2368933/76‑1 (MQ=255) gACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCa > 1:1864878/1‑76 (MQ=255) aCGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCAt > 2:266185/1‑76 (MQ=255) aCGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCAt < 2:4421426/76‑1 (MQ=255) gCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTg < 1:2922363/76‑1 (MQ=255) cTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGCTTCATCCCCAGCCCACTGGCATTgg > 1:8725931/1‑76 (MQ=255) cTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTgg > 1:3590391/1‑76 (MQ=255) cTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTgg > 2:7960609/1‑76 (MQ=255) tATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGtt < 1:381230/76‑1 (MQ=255) tATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGtt < 2:3510863/76‑1 (MQ=255) tATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGtt < 1:6557498/76‑1 (MQ=255) aTTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTa < 1:6569214/76‑1 (MQ=255) aTTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTa > 2:2261246/1‑76 (MQ=255) ttCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTAc > 2:5108718/1‑76 (MQ=255) ttCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTAc > 1:4846926/1‑76 (MQ=255) ttCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTGAc > 2:182824/1‑76 (MQ=255) ttCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATCCACCAGATTCATCCCCAGCCCACTGGCATTGGTTAc > 2:7016787/1‑76 (MQ=255) tCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTAcc > 1:1502702/1‑76 (MQ=255) ccATAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCg < 2:4784513/76‑1 (MQ=255) aTAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGt < 1:8239868/76‑1 (MQ=255) tAGTCATCGCCAATCCGTTCACGTAAACGCTTAGTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGtt < 1:263002/76‑1 (MQ=255) tAGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGtt < 1:2056145/76‑1 (MQ=255) aGTCATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTg < 1:2848729/76‑1 (MQ=255) cATCGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATa < 2:3846381/76‑1 (MQ=255) cGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACaa < 2:6206021/76‑1 (MQ=255) cGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACaa > 2:6071576/1‑76 (MQ=255) cGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACaa > 2:6959999/1‑76 (MQ=255) cGCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACaa < 1:5155167/76‑1 (MQ=255) gCCAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACaaa < 2:3117328/76‑1 (MQ=255) ccAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAAc < 1:807576/76‑1 (MQ=255) ccAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAAc < 1:6974202/76‑1 (MQ=255) cAAACCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAAcc < 2:789816/76‑1 (MQ=255) aaaCCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCg > 2:7579261/1‑76 (MQ=255) aaaCCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCg > 2:8298323/1‑76 (MQ=255) aaaCCGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCg < 1:7244050/76‑1 (MQ=255) aaCCGTTCACGTAAACGCTTATCTACCAGATTACTCCCGAGCCCACTGGCATTGGATAGTGGTTCATACAAAGCgg > 2:8342581/1‑76 (MQ=255) ccGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCGCTGGCATTGGTTACCGGTTGATACAAACCGGCa < 1:1680902/76‑1 (MQ=255) ccGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCa > 1:4619258/1‑76 (MQ=255) cGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCAt < 1:4946258/76‑1 (MQ=255) cGTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCAt < 2:5655375/76‑1 (MQ=255) gTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCAtt < 2:5033020/76‑1 (MQ=255) gTTCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCAtt < 2:4057874/76‑1 (MQ=255) ttCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTg < 2:6749004/76‑1 (MQ=255) ttCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTg > 1:8797240/1‑76 (MQ=255) tCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGt > 2:8895162/1‑76 (MQ=255) tCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGt > 2:337289/1‑76 (MQ=255) tCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGt > 1:4242910/1‑76 (MQ=255) tCACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGt < 1:3082749/76‑1 (MQ=255) cACGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTc < 1:3191480/76‑1 (MQ=255) cGTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCtt < 2:4649457/76‑1 (MQ=255) gTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTc < 2:5578212/76‑1 (MQ=255) gTAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAAACGGCATTGTCTTc > 2:3695174/1‑76 (MQ=255) ctAAACGCTTATCCTCCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTc < 2:6305869/75‑1 (MQ=255) tAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGGTACAAACCGGAGTTGTCTTcc > 2:1299386/1‑75 (MQ=255) tAAACGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCg < 1:1032962/76‑1 (MQ=255) aaaCGCTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGa > 1:7491597/1‑76 (MQ=255) cTTATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTcc > 1:2989376/1‑76 (MQ=255) ttATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCa > 2:2935725/1‑76 (MQ=255) tATTCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAg < 2:6659492/76‑1 (MQ=255) | CGGGTGTAACTATCAGGCTCACAGGCGACGCTTATTCCATAGTCATCGCCAAACCGTTCACGTAAACGCTTATCCACCAGATTCATCCCCAGCCCACTGGCATTGGTTACCGGTTGATACAAACCGGCATTGTCTTCGATCTCCAG > REL606/2164193‑2164338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |