| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,268,951 | T→G | I129S (ATC→AGC) | atoC → | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,268,951 | 0 | T | G | 99.6% | 931.1 / ‑5.0 | 225 | I129S (ATC→AGC) | atoC | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (112/112); total (113/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
Only 100 of 231 total aligned reads displayed.
AAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGAT > REL606/2268878‑2269023 | aaaCCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGcc > 1:2629660/1‑76 (MQ=255) aaCCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCg > 1:7503090/1‑76 (MQ=255) ccGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTc < 2:3841992/76‑1 (MQ=255) cGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCa > 2:183156/1‑76 (MQ=255) cGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCa < 1:5815656/76‑1 (MQ=255) tttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATc < 2:3328759/76‑1 (MQ=255) ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt < 2:6208768/76‑1 (MQ=255) ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt < 1:1117681/76‑1 (MQ=255) ttGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCt > 1:8685725/1‑76 (MQ=255) tGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTg > 2:4829872/1‑76 (MQ=255) tGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTg > 1:3571985/1‑76 (MQ=255) gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGc < 1:7637332/76‑1 (MQ=255) gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGc > 1:6203603/1‑76 (MQ=255) aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCa > 1:1544426/1‑76 (MQ=255) tctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAc < 2:7049610/76‑1 (MQ=255) tctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAc > 2:218287/1‑76 (MQ=255) ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:2571666/1‑76 (MQ=255) ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:7178281/1‑76 (MQ=255) ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCAcc > 1:5908348/1‑76 (MQ=255) tcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCa > 1:180832/1‑76 (MQ=255) cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg > 1:8171779/1‑76 (MQ=255) cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg > 2:1350787/1‑76 (MQ=255) cGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAg < 2:4652153/76‑1 (MQ=255) gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg < 1:6701225/76‑1 (MQ=255) gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg < 1:5133705/76‑1 (MQ=255) gATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACACCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAgg > 1:4600788/1‑76 (MQ=255) aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:7432305/1‑76 (MQ=255) aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:6809184/1‑76 (MQ=255) aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc > 1:7967890/1‑76 (MQ=255) aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc < 1:5367973/76‑1 (MQ=255) aTGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGc < 2:1323373/76‑1 (MQ=255) tGNNNNGAATTTAATCGTTCAGCGCGCTTTACAACNCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa > 2:4133173/1‑76 (MQ=255) tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa < 2:2913737/76‑1 (MQ=255) tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCa < 2:8279862/76‑1 (MQ=255) gAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCAc > 1:5482126/1‑76 (MQ=255) gAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCAc > 1:8456238/1‑76 (MQ=255) aGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACt > 1:6557186/1‑76 (MQ=255) tGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAg < 2:5589722/76‑1 (MQ=255) tttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCAcc > 1:8672382/1‑76 (MQ=255) tttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCAcc > 2:316040/1‑76 (MQ=255) ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa < 2:258107/76‑1 (MQ=255) ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa > 2:2317087/1‑76 (MQ=255) ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCa < 2:1276486/76‑1 (MQ=255) tAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAg > 1:120360/1‑76 (MQ=255) aTCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCt > 2:1138583/1‑76 (MQ=255) cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg > 2:815714/1‑76 (MQ=255) cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg < 2:7773869/76‑1 (MQ=255) cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTgg > 1:165143/1‑76 (MQ=255) ttCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCa > 1:1337281/1‑76 (MQ=255) tccAGCGCGCTTTCCAACTCCAGTCAAGGAAAAAAAAGAGCCGTCATCTGCTCCAGGCACTGAGCACCAGCTGGCa < 2:5479041/74‑1 (MQ=255) tCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCaa > 2:6246016/1‑76 (MQ=255) cAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAAt < 2:8710076/76‑1 (MQ=255) cAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAAt > 1:3022135/1‑76 (MQ=255) gcgcgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATgg < 2:847978/76‑1 (MQ=255) gcgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATgggg < 1:7215749/76‑1 (MQ=255) cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:8944602/76‑1 (MQ=255) cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 1:2880536/76‑1 (MQ=255) cgcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:1474186/76‑1 (MQ=255) gcTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc > 2:4295393/1‑76 (MQ=255) cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 2:6588714/76‑1 (MQ=255) cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 1:6585156/76‑1 (MQ=255) cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca < 1:3924184/76‑1 (MQ=255) cTTTACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGca > 1:2673706/1‑76 (MQ=255) tttACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac < 2:6972066/76‑1 (MQ=255) tttACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac > 1:5851680/1‑76 (MQ=255) tttACAACTCCAGTCAAGGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac < 1:2200078/76‑1 (MQ=255) tACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAt > 2:6876875/1‑76 (MQ=255) tACAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAt > 1:6412541/1‑76 (MQ=255) aCAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACAtt > 1:5232500/1‑76 (MQ=255) aCAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGAACAtt > 1:7828879/1‑76 (MQ=255) cAACTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtc > 2:4944852/1‑76 (MQ=255) aaCTCCAGTGAATGAAAAAAGAGAGCCGTCTTCTGCCCCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:316040/76‑1 (MQ=255) aaCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:8294691/76‑1 (MQ=255) aaCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:2225849/76‑1 (MQ=255) aaCCCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct < 1:4937733/76‑1 (MQ=255) aCTCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtctc < 2:8685725/76‑1 (MQ=255) tCCAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAc > 2:8355690/1‑76 (MQ=255) ccAGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAcc > 1:974484/1‑76 (MQ=255) aGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCaa > 1:4478700/1‑76 (MQ=255) aGTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCaa < 2:2827732/76‑1 (MQ=255) gTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAAc < 1:3218331/76‑1 (MQ=255) gTCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAAc > 1:3745816/1‑76 (MQ=255) tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa < 2:5415466/76‑1 (MQ=255) tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa > 1:433854/1‑76 (MQ=255) tCAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa < 2:6448892/76‑1 (MQ=255) cAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg > 1:1637801/1‑59 (MQ=255) cAATGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg < 2:1637801/59‑1 (MQ=255) aaTGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc < 1:6264139/76‑1 (MQ=255) aTGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGcc > 1:2067569/1‑76 (MQ=255) tGAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGccc < 1:7317072/76‑1 (MQ=255) gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 1:6079737/76‑1 (MQ=255) gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 2:7486950/76‑1 (MQ=255) gAAAAAAGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg < 2:5961939/76‑1 (MQ=255) aaaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCgg < 2:3441663/76‑1 (MQ=255) aaaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCgg > 1:4543831/1‑76 (MQ=255) aaaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGc < 1:4822702/76‑1 (MQ=255) aaaaGAGAGCCGTCATCTGCACGAGGCACTGAGCAACAGCTGGCAATGGGGGCACATGCTCACAAACAGCCCGTCg > 2:1887844/1‑76 (MQ=255) aaaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCg > 2:2387785/1‑76 (MQ=255) aaaGAGAGCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCga > 1:6785349/1‑76 (MQ=255) agagCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCgatgat > 2:3587057/1‑76 (MQ=255) | AAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGAT > REL606/2268878‑2269023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |