| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,326,724 | A→G | S15P (TCT→CCT) | mreD ← | cell wall structural complex MreBCD transmembrane component MreD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,326,724 | 0 | A | G | 98.8% | 1013.3 / 0.4 | 251 | S15P (TCT→CCT) | mreD | cell wall structural complex MreBCD transmembrane component MreD |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (137/111); total (138/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01 | |||||||||||
Only 100 of 272 total aligned reads displayed.
ACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTT > REL606/3326651‑3326797 | acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGACaggga > 1:2125917/1‑76 (MQ=255)acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:848508/76‑1 (MQ=255)acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:2781974/76‑1 (MQ=255)acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 2:6956901/76‑1 (MQ=255)acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAaggga < 1:3454891/76‑1 (MQ=255) cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAagggag < 2:3662796/76‑1 (MQ=255) cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc > 1:6848734/1‑76 (MQ=255) cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc > 1:8032336/1‑76 (MQ=255) cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGcc < 1:8874926/76‑1 (MQ=255) gTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAg < 1:3205770/76‑1 (MQ=255) gTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAg > 1:8403443/1‑76 (MQ=255) tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa > 2:5509054/1‑76 (MQ=255) tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa < 2:5955816/76‑1 (MQ=255) tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGa > 2:1927607/1‑76 (MQ=255) ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt > 1:463357/1‑76 (MQ=255) ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 1:5885943/76‑1 (MQ=255) ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 2:8222072/76‑1 (MQ=255) ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt > 2:7098853/1‑76 (MQ=255) ttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAt < 2:3184905/76‑1 (MQ=255) tGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGAtt > 1:2155015/1‑76 (MQ=255) ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa > 2:7845775/1‑76 (MQ=255) ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa > 2:5092718/1‑76 (MQ=255) ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTa < 1:7034313/76‑1 (MQ=255) gCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTAc < 1:8531951/76‑1 (MQ=255) ggAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCa < 1:5586544/76‑1 (MQ=255) gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAg < 2:2998952/76‑1 (MQ=255) gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAg > 1:1920687/1‑76 (MQ=255) aaaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGc > 2:1081428/1‑76 (MQ=255) aaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCCATGAGGAAAGGGAGCCAGATTACCCAGCg > 2:2684030/1‑76 (MQ=255) cAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTcc < 2:1226132/76‑1 (MQ=255) cAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTcc > 1:1091085/1‑76 (MQ=255) aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt < 1:1496730/76‑1 (MQ=255) aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt > 1:7379414/1‑76 (MQ=255) aTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCt < 1:6965582/76‑1 (MQ=255) tCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTg < 2:662395/76‑1 (MQ=255) cAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTgg > 1:2867018/1‑76 (MQ=255) cAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTgg > 1:6671108/1‑76 (MQ=255) ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCt < 2:5016740/76‑1 (MQ=255) gTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTa < 2:8895418/76‑1 (MQ=255) gTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTa < 2:6765198/76‑1 (MQ=255) ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCTTGGCTAc > 1:5729497/1‑76 (MQ=255) ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc < 2:7767748/76‑1 (MQ=255) ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc < 1:3047978/76‑1 (MQ=255) ttATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTAc > 2:8739795/1‑76 (MQ=255) tATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACg < 2:6229096/76‑1 (MQ=255) aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGa > 2:6475195/1‑76 (MQ=255) tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCGGGCTACGAt < 2:2118480/76‑1 (MQ=255) ccGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATa > 1:4523728/1‑76 (MQ=255) cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAg > 1:6747228/1‑76 (MQ=255) cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAg > 1:7882632/1‑76 (MQ=255) gCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCt > 1:6600647/1‑76 (MQ=255) cAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTcc > 2:8040173/1‑75 (MQ=255) aGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGc > 1:8350613/1‑76 (MQ=255) gCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCAc > 2:6108610/1‑76 (MQ=255) gCATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCAc > 1:7180946/1‑76 (MQ=255) cATGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACt > 2:4826802/1‑76 (MQ=255) aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTTGCCACTa > 2:7187443/1‑76 (MQ=255) aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCCCTa > 1:1773730/1‑76 (MQ=255) aTGATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTa > 2:4793232/1‑76 (MQ=255) gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt > 2:2731566/1‑76 (MQ=255) gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt < 2:3987467/76‑1 (MQ=255) gATTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAtt < 1:6827454/76‑1 (MQ=255) aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg < 2:2313583/76‑1 (MQ=255) aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg < 2:186/76‑1 (MQ=255) aTTTGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTg > 1:6392165/1‑76 (MQ=255) tttGCAGAAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACCATAGCTCGCCACTATTGc > 2:7479917/1‑76 (MQ=255) tGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGccc > 2:3593974/1‑76 (MQ=255) tGCAGCAACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGccc < 2:4362295/76‑1 (MQ=255) cggcaACAGCGCAAGGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTc < 1:599764/74‑1 (MQ=255) cagcaACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTc < 2:3098944/76‑1 (MQ=255) gcaACAGCGCAATGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTccc < 1:3581958/76‑1 (MQ=255) gcaACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTccc > 1:492003/1‑76 (MQ=255) caACAGCGCAATGAGGAAAGGGAGCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCg < 1:1331775/76‑1 (MQ=255) caACAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCg < 2:1468276/76‑1 (MQ=255) aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCgg > 1:4187216/1‑76 (MQ=255) aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCgg > 2:8420241/1‑76 (MQ=255) aaCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCgg < 1:1061663/76‑1 (MQ=255) aCAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgc < 1:5336512/76‑1 (MQ=255) cAGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcg > 1:6564168/1‑76 (MQ=255) aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcgc > 1:2726370/1‑76 (MQ=255) aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGgcgc < 2:34438/76‑1 (MQ=255) aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCCgcgc > 1:509027/1‑76 (MQ=255) aGCGCAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCATGGCTACGATAGCTCGCCACGATTGCCCTCCCGgcgc > 1:8933470/1‑76 (MQ=255) gcgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCa < 2:5226568/76‑1 (MQ=255) gcgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCa > 1:6243546/1‑76 (MQ=255) cgcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAc > 1:8191041/1‑76 (MQ=255) gcAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAcg > 1:7709948/1‑76 (MQ=255) cAATGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCAcgc < 1:1285676/76‑1 (MQ=255) aaTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCCTcccgcgcccgcg > 2:1623649/1‑76 (MQ=255) aggAGGAAGGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACgcgc < 1:42758/74‑1 (MQ=255) aTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACgcgc > 1:4803383/1‑76 (MQ=255) aTGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCCCCCGG‑GCAcgccaa > 1:3802591/1‑73 (MQ=255) tGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCa < 1:5249776/76‑1 (MQ=255) tGAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCa < 1:7944484/76‑1 (MQ=255) gAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAg < 1:873430/76‑1 (MQ=255) gAGGAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAg < 2:6670475/76‑1 (MQ=255) aGGAAAGGGACCCAGATTCCCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAgg < 2:2535304/76‑1 (MQ=255) gAAAGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCg > 1:755942/1‑76 (MQ=255) aaaGGGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCgg > 1:2626411/1‑76 (MQ=255) agagagCCAGCTTCCCCAGCGGCCCTGGCTACAATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGtt < 1:6916355/76‑1 (MQ=255) | ACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTT > REL606/3326651‑3326797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |