| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 98.4% | 742.3 / 2.7 | 183 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (69/111); total (70/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 232 total aligned reads displayed.
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑T‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 | aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑a < 1:8241792/76‑1 (MQ=255)aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑a > 1:7901960/1‑76 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑t > 2:6660277/1‑76 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tt < 2:705666/76‑1 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑ttt > 1:6465236/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑ttt > 1:7535998/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttt > 2:305319/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttt < 2:7193290/76‑1 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttt > 2:2156603/1‑76 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑ttttttt > 1:1867897/1‑75 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑ttttttt > 2:6884919/1‑75 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑ttttttt > 2:3609147/1‑75 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttc > 1:8078132/1‑74 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttc > 2:7943840/1‑74 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttc < 2:7879614/76‑3 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttc < 2:4018263/76‑3 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttc > 1:3995195/1‑74 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttca < 1:6995441/76‑4 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t‑tttttttca < 1:3623761/76‑4 (MQ=255) aCATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCg < 1:1100232/76‑1 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGt > 2:2350319/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTg < 2:2774950/76‑1 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGa > 1:4747818/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGa < 1:5194857/76‑1 (MQ=255) aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGaa < 2:5186448/76‑1 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGaaa < 1:5274347/76‑1 (MQ=255) ttcTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGa < 2:824423/74‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGa < 2:4279128/76‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGa > 1:8152649/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGa < 1:7768330/76‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGa > 2:5649932/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGAc < 1:4642351/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGAc < 2:450941/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGAc < 1:7877103/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGAc > 1:6023155/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGAc < 1:6424489/76‑1 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACa < 2:4224681/76‑1 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACa < 1:3158898/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACag < 2:3408493/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCATGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACag > 1:4246319/1‑76 (MQ=255) tGACTGGAATTTGAGAACGAATTTAAACGGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACag > 1:3798045/1‑76 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACaga < 1:145246/76‑1 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACagag < 1:4090816/76‑1 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACagag < 2:7367716/76‑1 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAgg < 2:483829/76‑1 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAgg < 2:3723901/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGa > 1:4145671/1‑76 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATa < 2:6522686/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATa > 2:8046875/1‑76 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaa < 2:8191546/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaaa < 1:2477330/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaaa < 1:913601/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaaa > 1:6073718/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaaa < 2:6931244/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATaaa < 1:6166125/76‑1 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAAt > 1:7971102/1‑76 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAAt < 1:4366326/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAAtt < 1:7588088/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAAtt < 2:1926266/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTg < 1:7407192/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTg > 1:2205796/1‑76 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTg > 1:8025513/1‑76 (MQ=255) gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGt > 1:6749965/1‑76 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 1:5051318/76‑1 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:6820975/76‑1 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa > 2:3529119/1‑76 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 2:911511/76‑1 (MQ=255) cGAATTTAAACCGCGTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 1:2075740/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:1654996/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:3292701/76‑1 (MQ=255) aagttAAACCGCTTCACCTTGAAAAAACTAC‑A‑TGTTTTTTCATCGTGAAATGGATAGAGGATAAATTGTTCAATaa < 2:8069960/73‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:3871089/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:7827614/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 1:5303623/76‑1 (MQ=255) aTTTAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc < 2:2644679/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 2:3202879/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 1:1168897/76‑1 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:6113524/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:6955739/1‑76 (MQ=255) aaaCCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 1:4174789/1‑76 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACTT‑T‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:6625146/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:5011486/76‑1 (MQ=255) cGCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 2:1843279/1‑76 (MQ=255) gCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc > 1:6806698/1‑76 (MQ=255) gCTTCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc < 2:7981337/76‑1 (MQ=255) tCACCTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACACAGGATAAATTGTTCAATAACGGATTATCCCCg < 1:7719989/76‑1 (MQ=255) cTTGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 1:4945406/76‑1 (MQ=255) ttGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 2:1046707/76‑1 (MQ=255) tGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 2:5254765/1‑76 (MQ=255) tGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:1389792/1‑76 (MQ=255) tGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:5423432/1‑76 (MQ=255) tGAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:2865785/1‑76 (MQ=255) gAAAAAACTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 2:2299637/76‑1 (MQ=255) aaaaaCTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:120792/76‑1 (MQ=255) aaaaaCTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:1562332/1‑76 (MQ=255) aaaaaCTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:6438704/76‑1 (MQ=255) aaaaCTTC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 2:1401833/76‑1 (MQ=255) aaaaCTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 1:2192782/76‑1 (MQ=255) aaaaCTAC‑ATT‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 2:5963558/76‑1 (MQ=255) aCTAC‑A‑T‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc > 2:7487833/1‑76 (MQ=255) | ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑T‑TTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |