| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 46.5% | 322.4 / inf | 389 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (165/43); new base T (181/0); total (346/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 425 total aligned reads displayed.
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC > REL606/1747828‑1747977 | ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:5767604/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:5560203/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:4948783/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:1672187/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:4018645/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:1070115/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:7904691/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:4740590/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:4312044/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:3802725/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:3343013/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:3540480/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAca > 1:4989249/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAca > 1:4131393/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:1281935/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:7454380/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:1136720/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:6269786/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:691212/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:1789050/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:371851/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACt > 2:1796369/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTG‑TTAATTCATTCAGGCATTACACGGTGGAAGCTTTTGGACTCCCGGCACtg > 1:3228438/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcct > 1:4698864/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcct > 1:2988073/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:1873794/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcat > 1:5649773/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcat > 1:2202385/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:6830228/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:6977220/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:7065859/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:6079721/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc < 1:4587696/76‑1 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:6681913/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:8133382/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:5838494/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:7345446/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:118713/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:5521098/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:7799325/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:19366/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:2138261/1‑74 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:2043838/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:4396716/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:4884245/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:1745359/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg < 2:2003394/76‑1 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc > 2:4409659/1‑76 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc > 2:7297963/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGccaat > 1:5288386/1‑73 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:5188453/1‑76 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:1537194/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACTTAGGCatat > 1:8266350/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 1:2265542/1‑76 (MQ=255) tatttatATTTTTTTGTTTTTTTAATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt < 1:7645129/76‑1 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACTTAGGCatatc > 1:3931212/1‑75 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt > 1:2771409/1‑76 (MQ=255) atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttt > 2:3867599/1‑76 (MQ=255) ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttttt > 2:6483254/1‑76 (MQ=255) ttttATTGTTTATTCCACGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt < 2:602839/76‑1 (MQ=255) tttattttttatttCATCCAGCCATTACACGATGAACGCTTATGAACCCCAGGCCCTCCGCCATATTTTTGCCATg < 1:1015115/76‑1 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:2583106/1‑76 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa > 1:5380670/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 2:274718/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 1:3394285/1‑76 (MQ=255) tttATTTCATCCAGCCATTACACGATGAACGCTTATGACCCCCAGGCACCCCGCCATATTTTTGCCATGACTATtg < 1:7984549/76‑1 (MQ=255) tttATTCCATCCAGCCATTACACGATAAACGTTTAGGACCCCCAGGCCCTCCGCCATATTTTTGCCATGACTATtg < 1:7298685/76‑1 (MQ=255) ttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 1:2839284/1‑76 (MQ=255) tATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:1465186/1‑76 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 2:4681232/1‑76 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:5910961/1‑76 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:6005190/1‑76 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa > 2:3344439/1‑76 (MQ=255) tCAGGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt < 2:5436367/76‑1 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg < 2:1268457/76‑1 (MQ=255) cATCCAGCCATTACACGATGAACGCTTATGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg < 1:4455482/76‑1 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 2:1117237/1‑76 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 1:2652638/1‑76 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 1:6460598/1‑76 (MQ=255) tccAGCCATTACACGATGAACGCTTATGACCCCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATgcg < 1:6190544/74‑1 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg > 1:6600371/1‑76 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 2:7565995/1‑76 (MQ=255) gCAGACATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 1:4280835/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 2:5875678/1‑76 (MQ=255) aTTACACGATGAACGCTTATGACCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg < 2:5547526/76‑1 (MQ=255) aTTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg > 1:3682727/1‑76 (MQ=255) acacGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt > 1:738949/1‑76 (MQ=255) cacGATGAACTCTTATGAACCCCAGCCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt < 1:4170826/76‑1 (MQ=255) ataaaCGTTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 2:5189595/73‑1 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc > 2:366175/1‑76 (MQ=255) tGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa < 2:6922718/76‑1 (MQ=255) aaCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa > 2:4012744/1‑76 (MQ=255) aaCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa > 1:7151918/1‑76 (MQ=255) cGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 2:2262790/76‑1 (MQ=255) gtttATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg < 2:4280835/74‑1 (MQ=255) cTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg > 1:6505905/1‑76 (MQ=255) ttATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt > 2:6665663/1‑76 (MQ=255) ccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa > 2:7801113/1‑76 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTCACAGTGaaa > 2:4935795/1‑76 (MQ=255) aGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAg < 2:161621/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC > REL606/1747828‑1747977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |