| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 44.0% | 87.2 / 27.1 | 75 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/27); new base C (0/33); total (15/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 115 total aligned reads displayed.
CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTA > REL606/1854326‑1854473 | ctgtCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCaaaa‑aa < 2:475233/73‑1 (MQ=255) tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGGGGGGTGGCCaaaa‑aaa > 1:5700950/1‑75 (MQ=255) tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑c < 1:993996/76‑1 (MQ=255) tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑c < 1:2900167/76‑1 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑Ca < 1:7996846/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGc < 1:1698280/76‑1 (MQ=255) gCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTg < 1:3574431/76‑1 (MQ=255) aaTATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGGGGCACACCCAAAA‑AA‑AAACCGCCGAc > 1:8079821/1‑76 (MQ=255) aaTATCTTGCAGAACGGGCGAAACACGAGCAAGAAGAAAGGGGTCGGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGAc < 2:2865468/76‑1 (MQ=255) aaTATCTTGAAGACCTGGCGCAACACGAGAAAGAAGGAAAGGGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGAc < 2:2689205/76‑1 (MQ=255) tatCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGAccc < 1:832573/76‑1 (MQ=255) tccTTGCAGCCCGGGCGGAAAACGACCAAGAAGGAAAGCGGTGGGCGGAAAGCGCAAAAA‑AC‑CAGCCGCTGACCCt < 2:5861525/74‑1 (MQ=255) atCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCt < 1:1490487/76‑1 (MQ=255) atCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCt < 1:3911003/76‑1 (MQ=255) atCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCt < 1:7957931/76‑1 (MQ=255) tCTTGCAGAACGGGCGCAACACGAGCAAGAAGAAAAGCGGCGGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTg < 2:2936530/76‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGAAAGGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGctct < 2:6736090/76‑1 (MQ=255) cAGACCTGGCGCAACAGGAGAAAGAAGGAAGGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTa < 1:7613149/76‑1 (MQ=255) aGAACGGGCGCAACACGAGCAAAAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTAc < 1:1009726/76‑1 (MQ=255) gAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACg < 2:7370990/76‑1 (MQ=255) gAACTGGCGAAACACGAGCAAGAAGGAAGGCGGCGGGCGGACCTCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACg < 2:155535/76‑1 (MQ=255) aaaCGGGCGCAACACGAGAAAGAAGGAAAGCGGCTGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACg < 2:214739/75‑1 (MQ=255) aaCGGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACgc < 1:2933049/76‑1 (MQ=255) caggCGCAAGACGAGCAAGAAGGAATGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACgcag < 1:7278023/74‑1 (MQ=255) cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGGGGCATCGCCCAAA‑AA‑CAAACCCCGCGCCTGCGCTACgcag > 1:8250232/1‑76 (MQ=255) tGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACgcagc < 1:8107696/76‑1 (MQ=255) ggCGCAACACGAGCAAGAAGAAAAGCGGCTGGCGAACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACgcagca < 1:5250415/76‑1 (MQ=255) cgcAACAGGAGCAAGAAGGAAGGCGGCTGGCGGACACCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCaaa < 2:655166/76‑1 (MQ=255) cgcAACACGAGCAAGAAGGAAAGCGGCGGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:5449943/76‑1 (MQ=255) cgcAACACGAGAAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCaaa < 2:5172817/76‑1 (MQ=255) cacAACACGAGCAAGAAGGAAAGGGGCGGGCGGACATCACAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCaaa < 2:3120724/74‑1 (MQ=255) gcAACACGAGCAAGAAGGAAAGCGGCTGGGGGGGAACGCCCAAA‑AA‑CAAACGCTGCCCCCCCCCTCCGCCGCaaaa > 1:2686482/1‑76 (MQ=255) catcacGAGCAAGAAGGAAAGCGGCTGGCGAACTTCGCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 2:7288578/73‑1 (MQ=255) cccGAGAAAAAGGGAAAGGGGCGGGCGGACATCCCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 1:4925349/74‑1 (MQ=255) cacGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 1:2674913/76‑1 (MQ=255) acGAGCAAGAAGGAAAGCGGCTGGGGGGCATCGCCAAAA‑AA‑CAACCGCCCACCCCCCTCTGCCCACCAAAAAaaac > 2:4923207/1‑76 (MQ=255) acGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAacac < 1:5394183/76‑1 (MQ=255) cgcgCAAGAAGGAAAGCGGCGGGCGGACATGCCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 2:1070333/73‑1 (MQ=255) gagaAAGAAGGAAAGCGGCGGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacca < 2:1306139/76‑1 (MQ=255) gAGCAAGAAGGAAGGCGGCTGGCGGACATCGCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacca < 2:1039345/76‑1 (MQ=255) ggaaagaagGAACGCGGCTGGCGGAACCCGAAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcaccac < 2:454344/73‑1 (MQ=255) gacAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcaccac < 1:5924980/74‑1 (MQ=255) aggaagaagGGAGGGGGGTGGCCGACGTGGCAGAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcaccac < 2:6011758/73‑1 (MQ=255) gaaagaagGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 1:1160820/74‑1 (MQ=255) gCAAGAAGGAAAGCGGCTGGGGGGCCACGCCCAAA‑AA‑AAACCCCCGGCCCCGCCCTACCGCGGAAAAAAaaccaca > 1:5628817/1‑75 (MQ=255) gCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 1:1454721/76‑1 (MQ=255) cAAGAAGAAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 1:1678892/76‑1 (MQ=255) aaagaggGAAAGCGCCGGGCGGACATCCCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 2:7469100/75‑1 (MQ=255) aaagaagAAAGGGGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 2:1499938/75‑1 (MQ=255) aagaagGAAAGCGGCTGGGGGGCAGCGCCAAAA‑AA‑AAGCCGCCGACCCCGCTCTTCCCCGCCAAAAAcaacaccga > 1:6214555/1‑73 (MQ=255) aagaagGAAAGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:5861210/76‑1 (MQ=255) aagaagGAAAGCGGCGGCCGAACACCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:579434/76‑1 (MQ=255) gaagaaAGGCGGCTGCCGGCAGACGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 2:1567258/76‑1 (MQ=255) ggAAAGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 1:3400463/76‑1 (MQ=255) aaaaGCGGCTGGCGGACATCGCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCa < 2:3098303/75‑1 (MQ=255) aaaGCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:2918591/76‑1 (MQ=255) aaaGCGGCGGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:8101516/76‑1 (MQ=255) aaaGCGGCGGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:8091319/76‑1 (MQ=255) acgCGGCTGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 2:478762/74‑1 (MQ=255) ggcggcgGGCGGACATCGCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 2:7273242/75‑1 (MQ=255) gcggcgGGCGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 2:3472772/76‑1 (MQ=255) gcggcgGCCGAACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 2:3097083/76‑1 (MQ=255) gCGGCTGGCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 2:3886996/76‑1 (MQ=255) ggCTGGCGGACATCGCCCAAA‑AA‑CACCCGCTTAACCTTTTCTTGCCACACAAAAACACCACGCAGAAACATgcccc > 2:6076041/1‑73 (MQ=255) gcgggCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 1:2670956/73‑1 (MQ=255) cTGGGGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg < 1:5838668/76‑1 (MQ=255) tGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 2:5688287/1‑76 (MQ=255) tGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 2:4525720/1‑76 (MQ=255) gggCGAACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 2:2676928/75‑1 (MQ=255) ggCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg > 2:2685668/1‑76 (MQ=255) ggCGGACATCGCCAAAA‑AA‑CAGACGCTGACCCTGTTCTACGCAGTAAAAAACACCACGCAGAACCATGCGCTGGTg > 2:1482133/1‑76 (MQ=255) ggCGGACATCCCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 1:2448570/76‑1 (MQ=255) ggggACCTCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc < 2:6567163/74‑1 (MQ=255) gCGGACATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc < 1:4550899/76‑1 (MQ=255) gCGGACATCCCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc < 2:4888939/76‑1 (MQ=255) cGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 1:3117228/1‑76 (MQ=255) cGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:56619/1‑76 (MQ=255) cGGACATCGCAAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 1:7259852/76‑1 (MQ=255) ggACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 2:2128770/76‑1 (MQ=255) gcacacCGCAAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 1:4671967/74‑1 (MQ=255) ccATCGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc < 2:7600960/75‑1 (MQ=255) cATCGCCAAAA‑AC‑CCGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc < 1:2080313/76‑1 (MQ=255) cATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 1:1254937/1‑76 (MQ=255) accGCCAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg < 1:6795871/74‑1 (MQ=255) aTCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCCGAACCATGCGCTGGTGCTGGCCg > 1:2240594/1‑76 (MQ=255) aTCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 1:451307/1‑76 (MQ=255) cGCCAAAACAT‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAACCGCCACGCAGAACCATGCGCTGGTGCTGGCCGa < 2:469124/76‑1 (MQ=255) gcaaaaa‑ac‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 2:4007538/73‑1 (MQ=255) ccaaaaa‑ac‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 2:5702860/73‑1 (MQ=255) ccAAAA‑CC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCGGGTGCTGGCCGACTg < 2:7466432/76‑1 (MQ=255) ccAAAA‑ACCCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACGCCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:6819979/76‑1 (MQ=255) ccAAAA‑AC‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 2:360412/76‑1 (MQ=255) ccAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 2:4147566/1‑76 (MQ=255) cAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCTGACTgg > 1:2000172/1‑76 (MQ=255) aaaa‑aa‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGc > 2:357965/1‑76 (MQ=255) aaa‑ac‑CAGCCGCTTACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:6895467/76‑1 (MQ=255) aaa‑ac‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:6251652/76‑1 (MQ=255) aaa‑aa‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt > 2:4747752/1‑76 (MQ=255) aa‑ac‑CAGCCGCTGGCCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:1306871/76‑1 (MQ=255) aa‑aa‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:6330769/1‑76 (MQ=255) | CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAA‑AA‑CAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTA > REL606/1854326‑1854473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |