| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433 | 0 | T | G | 44.9% | 111.1 / 41.5 | 89 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base T (12/37); new base G (39/1); total (51/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 180 total aligned reads displayed.
GTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCC > REL606/358‑507 | gTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGt < 1:6756500/76‑1 (MQ=255)gTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGt < 2:664108/76‑1 (MQ=255) cATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCC‑Gggggg > 2:4987103/1‑75 (MQ=255) cATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑Gggggg > 2:2940756/1‑75 (MQ=255) aTCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑Ggggggc > 1:6337378/1‑74 (MQ=255) aTCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGcc < 2:4574192/76‑1 (MQ=255) aTCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGcc < 1:7985518/76‑1 (MQ=255) tCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCa < 2:5367664/76‑1 (MQ=255) tCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCa < 2:6717824/76‑1 (MQ=255) aGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCAcc < 2:2805675/76‑1 (MQ=255) aGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCAcc > 2:6541649/1‑76 (MQ=255) tGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCGCCgc > 2:4807823/1‑75 (MQ=255) tGGCAAATGAAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGt < 2:7331040/76‑1 (MQ=255) cAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCct < 2:4552899/76‑1 (MQ=255) cAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCct < 1:1877957/76‑1 (MQ=255) aTGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCctctc < 2:7035695/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCctctct < 1:2493000/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCctctct < 2:2439890/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCctctct < 1:5573993/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCctctct < 2:7028719/76‑1 (MQ=255) gCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTg < 2:7413155/76‑1 (MQ=255) gCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTg < 2:3306943/76‑1 (MQ=255) gCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCCCCccctctc > 2:797924/1‑75 (MQ=255) gCAGAACGTTTCCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTg < 1:3289036/76‑1 (MQ=255) gAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGccc < 1:7213316/76‑1 (MQ=255) aCGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGccccc < 1:5090803/76‑1 (MQ=255) aCGTTTGCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGccccc < 2:136898/76‑1 (MQ=255) cGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCg < 2:1615844/76‑1 (MQ=255) cGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGTCCTCTCTGcccccc > 2:3590366/1‑75 (MQ=255) ttttCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCCCCGTCCCCTCTTcccccccc > 1:6759935/1‑76 (MQ=255) tttcCTGCGGGTT‑GCGGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGcc < 2:7464633/76‑1 (MQ=255) ttCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGGGCCCCCCCCTCTGCCCCCGcccc > 1:6457123/1‑74 (MQ=255) ttCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCCTCCCCTCTGccccccccca > 1:4514552/1‑74 (MQ=255) ttCGGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCaa < 1:7451852/76‑1 (MQ=255) tCTGCGGGTT‑GCGGATATTCGGGAAAGCAATGCCGGGCA‑GGGGCC‑GGCGCCCCCCGTCCCCTCTGCCCCCGCCaaa < 2:6955676/76‑1 (MQ=255) tCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCCCCGCCCCCTCTTCCCCCGCCcaa > 1:3239589/1‑76 (MQ=255) tCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGCCACCCCTGCCCCCCCCcaa > 1:6983290/1‑76 (MQ=255) tCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGCCACCGGCCTCTCTTcccccccccca > 2:5254788/1‑73 (MQ=255) cTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGCCCTCCCTCCCCCCCCCacaa > 1:3592169/1‑76 (MQ=255) tGCGGGTG‑CCCGATATTCGGGAAAGCAATGCCAGGCA‑GGGGCG‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAAt < 1:1634570/76‑1 (MQ=255) gCGGTTT‑GCCGATATTCTGGAAAGCAATCCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATc < 1:4826947/76‑1 (MQ=255) cGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGGACCGGTCTCTCTTCCCCCGCCcaaaaaa > 1:2843103/1‑73 (MQ=255) cGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGGCCTCTCTGCCCCCGCCCAAAACa > 2:1747053/1‑76 (MQ=255) gggTTGGCCGATATTCGGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGCCCACCGTCCTCTCTGCCCCCGCCAAAATCa < 2:394542/76‑1 (MQ=255) ggTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCacc > 2:4301909/1‑76 (MQ=255) gTT‑GCTGATATTCTGGAAAGCAATGCCAGGCG‑GGGGCA‑GGTGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCacca < 1:7735370/76‑1 (MQ=255) tt‑GCCGATATTCTGGAAAGAAATCCCAGGCG‑GGGGCG‑GGTGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCaccaa < 1:2345123/76‑1 (MQ=255) t‑GCCGATATTCTGTAAAGAAATGCCAGGGG‑GGGGCG‑GGGGGCCTCCGTCCTCTCTGCCCCCGCCAAAATCaccaac < 2:6058280/76‑1 (MQ=255) t‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGCCCCCCTCCCCTCTTCCCCCCCCAAAATAaccaac > 1:106014/1‑76 (MQ=255) t‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGCCACCGTCCTCTCTGCCCCCGCCAAAATCaccaac > 1:1933725/1‑76 (MQ=255) t‑GCCGATATTCTGGAAAGCAAGGCCAGGCA‑GGGGCA‑GGGGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCaccaac < 1:70562/76‑1 (MQ=255) cGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCCCCCAAATCcccaacccc > 1:3995933/1‑74 (MQ=255) gATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGTCCTCTCTTCCCCCCCCCAAATCCCCCaccacc > 1:1530424/1‑76 (MQ=255) atatTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCCCCCTCCTCTCTTCCCCCCCCCAAATCCCCAACCACCt > 2:3120609/1‑76 (MQ=255) atatTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGCCCCCCTGCTCCTTCCCCCCCCCCACAAGACCCaccaccc > 1:1260143/1‑75 (MQ=255) ttttCTGGAAAG‑AATGGCCGGCAGGGGGCA‑GGGGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtg < 2:4495428/74‑1 (MQ=255) tatTCTGGAAAGCAAGGCCAGGCG‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtg < 2:1699705/76‑1 (MQ=255) atTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCCCCCCCCCCTCTCCCCCCGCCAAAAAAACCAACCACCtcg > 2:4417711/1‑74 (MQ=255) atTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCCCCATCCTCTCCTCCCCCGCCAAAAACAACCACCCGCtgg > 1:2240664/1‑76 (MQ=255) ttCTGAAAAGAAAGGCCGGGCG‑GGGGCG‑GGTGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtggt < 2:7088870/76‑1 (MQ=255) tcgggAAGGCATTGCAGGGAA‑GGGGTA‑GGTGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtggtg < 1:7191128/73‑1 (MQ=255) tCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCGGCCTCTCTGCCCCCGCCAAAAACAACCACCACCtgggg > 2:6075971/1‑74 (MQ=255) tCTGAAAAGCAAGGCCAGGCA‑GGGGAA‑GGTGGCCACCGTCCTCTCTCCCCCCGCCAAAATCACCAACCACCtggtg < 2:5495873/76‑1 (MQ=255) tGGAAAGCAATGCCAGGCG‑GGGGCA‑GGGGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGc < 2:2888832/76‑1 (MQ=255) tGGAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCCTCCCCTCTGCCCCCCCCAAAAACACCCACCACCTGGTGGc > 1:4758315/1‑76 (MQ=255) tGGAAAGCAAGGCCGGGCG‑GGGGCG‑GGTGCCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGc < 1:7472474/76‑1 (MQ=255) ggAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGGCACCCTCCTCCCTGCCCCCCCCAAAAACACCAACCACCCGGTGGCg > 2:7552697/1‑76 (MQ=255) ggAAAGCAATGCCAGGCA‑GGGGCA‑GGGGGCCACCGTCCTCCCTTCCCCCGCCCAAAAAACCAACCACCtggggggg > 2:2997679/1‑74 (MQ=255) aaaGCAAGGCAGGGAA‑GGGGGA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgat < 2:591548/76‑1 (MQ=255) aaGAAATGCCAGGCA‑GGGGGG‑GGGGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgatg < 2:8230485/76‑1 (MQ=255) aacAATGCCGGGCG‑GGGGCA‑GGTGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgatga < 1:6188300/74‑1 (MQ=255) aGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCt > 2:2030073/1‑65 (MQ=255) gCAATGCCAGGCA‑GGGGCA‑GGGGGCCCCCGTCCTCTCTCCCCCCGCCAAAATCACCACCCACCCGGTGGCgatgat > 2:3982730/1‑76 (MQ=255) cAATGCGAGGCA‑GGGGCA‑GGGGGCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGAtt < 1:6160570/76‑1 (MQ=255) aaTGCCGGCCG‑GGGGCG‑GGTCCCCCCCGTCCTCTCTCCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTg < 1:4306308/76‑1 (MQ=255) aaTGCCAGGCA‑GGGGCC‑GGGGGCCCACGTCCTCCCTGGCCCCCCCAAAAAAAACAAACACCCGGAGGCGATGAGTg > 2:277535/1‑76 (MQ=255) agcccAGGCG‑GGGGCA‑GGGGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGa < 2:7802108/73‑1 (MQ=255) aagCCCGGCC‑GGGGCCGGGTGCCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTg < 1:4802347/74‑1 (MQ=255) tGCCAGGCA‑GGGGCA‑GGGGGGCACCGCCCCCTCTGCCCCCCCCAAAAACACCAACCACCCGGGGGCGGTGGTTGaa > 2:4608739/1‑76 (MQ=255) gCCGGGCG‑GGGGCG‑GGGGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGaaa < 1:5668143/76‑1 (MQ=255) gCCAGGCA‑GGGGCA‑GGGGGGCCCCGTCCTCTCTGCCCCCGCCCAAAACACCAAACACCTGGTGGCGATGATTTaaa > 2:5980504/1‑76 (MQ=255) gCCAGGCA‑GGGGAA‑GTTGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGaaa < 2:6186738/76‑1 (MQ=255) ccAGGCA‑GGGGCA‑GGGGGGCCCCCCCCCCTCTTCCCCCCCCAAAAACACCCACCACCCCGGGGGGATGATTTaaaa > 2:7505221/1‑76 (MQ=255) ccAGGCA‑GGGGCA‑GGGGGGCACCGTCCTCTCTCGCCCCGCCAAAAACACCAACCACCTGGGGGCGAGGATTTaaaa > 2:1078479/1‑76 (MQ=255) caggca‑ggGGCA‑GGGGGCCACCCGCCTCTCTGCCCCCCCCAAAAAAACCAAACACCCGGGGGCGGTGAATTaaaaa > 2:6723638/1‑76 (MQ=255) aggca‑ggGGCA‑GGGGGGCACCGTCCTCTCTTCCCCCGCCAAAATCACCAACCACCCGGTGGCGGTGATTGaaaaaa > 2:2618211/1‑76 (MQ=255) aggca‑ggGGCA‑GGGGGGCACCGTCCCCTCTGCCCCCCCCAAAAACACCAACCACCTGGTGGCGGTGATTGtaaaaa > 2:57462/1‑76 (MQ=255) ta‑ggggca‑ggGGGGCACCGTCCTCTCTTCCCCCGCCAAAAACACCAACCACCTGGTGGCGATGATTTAAAAAAcaa > 2:1920165/2‑74 (MQ=255) ta‑ggggca‑ggGGGGCACCGCCCTCTCTGCCCCCCCCAAAATCACCAACCCCCCGGTGGCGATGATTGTAAAAAACa > 2:272695/2‑76 (MQ=255) gagggggcg‑ggCGCCCCCCGTCCTCTCTCCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAAcc < 1:490148/73‑1 (MQ=255) ca‑ggggca‑ggggGGCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAAccc > 2:2395827/1‑75 (MQ=255) ca‑ggggca‑ggggGGCACCGTCCTCTCTGCCCCCCCCAAAATCAACAACCACCTGGGGGCGATGATTGAAAAAAACa > 2:1048400/1‑76 (MQ=255) ca‑gggccg‑ggTGCCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCa < 1:1169446/76‑1 (MQ=255) a‑ggggca‑ggGGGGCCCCCCCCTCTCTGCCCCCCCCCAAAAAACCAACCACCCGGGGGCGCTGATTGAAAAAAACaa > 2:4854215/1‑75 (MQ=255) ggggaa‑ggGGGCCCCCGTCCTCTCTGCCCCCTCCAAAAACACCAATCACCTCGGGGCGCAGACTTAAAAACACAtt > 2:2180537/1‑76 (MQ=255) gggcg‑ggTGCCCCCCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTa < 1:6563881/76‑1 (MQ=255) gggcg‑ggTGCCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTa < 2:7171309/76‑1 (MQ=255) a‑ggTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCgg > 1:927062/1‑76 (MQ=255) ggTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGc > 1:2892986/1‑76 (MQ=255) gTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGACGATTGAAAAAACCATTAGCGGcc > 2:3291908/1‑76 (MQ=255) | GTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTT‑GCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCA‑GGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCC > REL606/358‑507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |