| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,595,070 | 0 | A | G | 36.4% | 283.4 / 108.1 | 173 | G11G (GGA→GGG) | yjjZ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/98); new base G (63/0); total (75/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-35 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 251 total aligned reads displayed.
TCATATGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATT > REL606/4594995‑4595145 | tatGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAgtg < 1:3159644/76‑1 (MQ=255) atGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGgtgt > 1:1776505/1‑76 (MQ=255) gATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTtg < 1:2018640/76‑1 (MQ=255) atatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTtgc > 1:6528592/1‑76 (MQ=255) tatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTtgct > 1:5963414/1‑76 (MQ=255) tatTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTtgct < 2:1857351/76‑1 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTtgctg > 2:4679036/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTtgctg > 2:4236263/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTtgctg > 2:3248391/1‑76 (MQ=255) atTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGtgctg > 1:6245701/1‑76 (MQ=255) ttGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTtgctgc > 2:7683633/1‑76 (MQ=255) tGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGcc < 1:1629753/76‑1 (MQ=255) tGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGcc < 1:2740251/76‑1 (MQ=255) tGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGcc < 1:3659262/76‑1 (MQ=255) ggTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCg > 2:5868704/1‑76 (MQ=255) ggTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCg > 1:281717/1‑76 (MQ=255) ggTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCg > 1:8205905/1‑76 (MQ=255) ggTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCg < 1:8002885/76‑1 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCgg > 2:650469/1‑76 (MQ=255) gTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGGTGCCgg > 1:1904741/1‑76 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGGCggg > 2:3378927/1‑76 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCggg < 1:6297015/76‑1 (MQ=255) ttatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGGGTTGCTGCCggg > 1:2557313/1‑76 (MQ=255) tatcattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCcggg > 2:2290141/1‑75 (MQ=255) tattatcaATGAGAGAGATGAAAACATGTTGCAGCGTGCGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTg < 2:3261235/75‑1 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCACCGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTg < 2:3002499/76‑1 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGTTGCCGGGGTTg > 2:2664244/1‑76 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTg < 2:1396424/76‑1 (MQ=255) cattatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTg < 1:6376331/76‑1 (MQ=255) attatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGc > 2:4442464/1‑76 (MQ=255) attatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCGGGAGTGc > 1:2199926/1‑76 (MQ=255) attatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGAGCTGCTGCCCGGGTTgg > 2:5606171/1‑75 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGGTTGCt > 2:3888232/1‑76 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGGCGGGGGTGCt > 1:8025243/1‑76 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTggt > 1:950933/1‑74 (MQ=255) ttatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCt < 2:3854675/76‑1 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAGCGTACGCTGGGCAGTGGCTGGGGGGGGGTTCTTGCCGGGATTggt > 2:6579103/1‑74 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCCGGGTTGGTg > 1:1299297/1‑76 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTg < 1:244342/76‑1 (MQ=255) tatcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTg < 1:6844040/76‑1 (MQ=255) atcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGGTGCTGCCGGGGTTGGTGa > 1:7850395/1‑76 (MQ=255) atcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGAGTGCTgg > 2:905971/1‑75 (MQ=255) tcaATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGGGGTGCTGCCGGGAGTGCTggt > 2:6962293/1‑74 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGaat > 1:1316206/1‑74 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGGTGCTGAtt > 2:2325668/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCGGCCTGGGTGGCTGGtt > 1:4058278/1‑76 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGAtt < 1:7610826/76‑1 (MQ=255) caATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGAtt < 2:6192377/76‑1 (MQ=255) aaTGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTg > 1:4866774/1‑76 (MQ=255) aaTGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCGGGGTTGCTGATTg > 1:3419771/1‑76 (MQ=255) tGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGAtttcc > 2:4337561/1‑73 (MQ=255) tGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGGTGCCGGGATTGCTGATTGcg > 1:2932756/1‑75 (MQ=255) tGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGGGTTGGTGCCGGGATTGCTCATTGCa > 2:1709689/1‑76 (MQ=255) gagagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAggg < 2:5829379/76‑1 (MQ=255) agagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTTCAgggg > 1:5419374/1‑75 (MQ=255) agagaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGAGTTGCTGCCGGGAGTGCTGATTGCAgagg > 2:8104382/1‑73 (MQ=255) agaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGGTTGGTGTTTTCAGGGCtc > 1:8127874/1‑75 (MQ=255) agaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGAGTGCTGATTGTAggggag > 2:7891891/1‑73 (MQ=255) agaTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCGGGGGTGGTGGTTGCAGGGccg > 1:1008066/1‑74 (MQ=255) aTGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCGGGGTTGCTGATTGCCGGGGTggt > 1:7931805/1‑74 (MQ=255) aTCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATg < 2:7317062/76‑1 (MQ=255) tCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGc < 1:3619287/76‑1 (MQ=255) cATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGATTGCTGATTGCAGGGGTGGGGTATGCg > 1:5749188/1‑76 (MQ=255) cATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCg < 2:2495356/76‑1 (MQ=255) aTGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGGTTGCTGATTGCCGGGCTGGTGTATGCgg > 2:1896932/1‑76 (MQ=255) aTGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGGTGTATGCgg > 2:6311618/1‑76 (MQ=255) tGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTGTAGGGGTGGTGTATGTggg > 1:6689302/1‑75 (MQ=255) ttGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGAtt < 1:2180022/76‑1 (MQ=255) tGCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGGTTGCAGGGCTGATGTATGCGGGttt > 1:7062873/1‑76 (MQ=255) gCAACGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGGTTGCAGGGCTGATGTATGCGGGtttt > 1:7304910/1‑75 (MQ=255) gCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTa < 2:5433681/76‑1 (MQ=255) aaCGTACGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGATTGCTGATTGCAGGGGTGGTGGATGCGGGTTTATc > 1:3237256/1‑76 (MQ=255) aaCGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGGTTGCAGGGCTGGTGGATGCGGGTTTAtt > 1:2117686/1‑75 (MQ=255) aaCGTACGCTGGGCAGTGGCT‑GGGGGGGGGTGCCGGCGGGGTTGGTGATTGCAGGGCCGGTGTATGCGGATTTTTc > 2:6390020/1‑76 (MQ=255) aCGTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGATGCTGATTGCAGGGGTGATGTATGCGGATTTATCg > 2:8162727/1‑76 (MQ=255) gTACGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGGTTGCAGGGCTGATGTATTCGGGTTTTTCGTc > 2:6944304/1‑76 (MQ=255) aCGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGGTTGCTGGTTGCAGGGCTGGTGGATGCGGATTTATTGTCAg > 2:2765036/1‑76 (MQ=255) aCGCTGGGCAGTGGCT‑GGGGGGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATTGTCaa > 2:2166185/1‑75 (MQ=255) aCGCTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATTCGGGTTTTTTGTCAg > 2:7165355/1‑76 (MQ=255) aCGCTGGGCAGTGGCT‑GGGGGGGGGTGCTGCCGGGGGTGGTGATTGCAGGGGCGACGTATGCGGACTTTTTGTccg > 2:6686811/1‑74 (MQ=255) gCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGAGTGCAGGGCTGATGTATGCGGATTTATCGTCAGAt < 1:53913/76‑1 (MQ=255) cTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTTCAGGGGTGGTGTATGCGGGTTTTTCGTCCGAta > 2:371355/1‑75 (MQ=255) cTGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGATTGCTGATTGCAGGGGTGATGTATGCGGATTTATCGTCAGATc > 2:1090050/1‑76 (MQ=255) cTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATc < 2:7591305/76‑1 (MQ=255) tGGGCAGTGGCT‑GGGGGGGGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCa > 2:4005326/1‑76 (MQ=255) tGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCa < 1:4704930/76‑1 (MQ=255) tGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCa < 2:1171726/76‑1 (MQ=255) gggCAGTGGCT‑GGGGGGGGTTGCTGCCGGGGTTGCTGATTGCCGGGGTGATGTATTCGGATTTATCGTCAGATCAg > 2:491946/1‑76 (MQ=255) gggCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAg < 1:7492099/76‑1 (MQ=255) aGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGc < 1:3590079/76‑1 (MQ=255) tGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCgg < 1:7940547/76‑1 (MQ=255) tGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCgg < 1:902438/76‑1 (MQ=255) ggCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCggg > 2:1841047/1‑75 (MQ=255) gCT‑GGGGATGGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAt < 2:6291154/76‑1 (MQ=255) t‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTg > 1:3772045/1‑76 (MQ=255) ggggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGGTTGt > 2:3752753/1‑76 (MQ=255) ggggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGt > 2:6054638/1‑76 (MQ=255) ggggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGt < 2:3122456/76‑1 (MQ=255) ggAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGGTTGGCa > 2:7801378/1‑76 (MQ=255) aGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAtt < 2:352640/76‑1 (MQ=255) | TCATATGATATTGGTTATCATTATCAATGAGAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCT‑GGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATT > REL606/4594995‑4595145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |