BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,498,410	A→C	S360A (TCG→GCG)	glgA ←	glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,498,410	0	A	C	100.0%	689.2 / NA	168	S360A (TCG→GCG)	glgA	glycogen synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (90/78); total (90/78)

Only 100 of 226 total aligned reads displayed.

AGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTC  >  REL606/3498337‑3498482

aGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCCCCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGcaa                                                                        <  1:5180987/76‑1 (MQ=255)

aGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGcaa                                                                        <  1:2116621/76‑1 (MQ=255)

  ttGCGTTAAGCCGCACGGTTCAAAACGGCTGGGGACCAGAATGACGTCCGCGCCGCCCATAATGCGATGGGCAAAt                                                                      >  2:4608195/1‑76 (MQ=255)

  ttGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAAt                                                                      <  1:5401058/76‑1 (MQ=255)

  ttGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAAt                                                                      >  1:2335978/1‑76 (MQ=255)

  ttGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAAt                                                                      >  1:2951107/1‑76 (MQ=255)

   tGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATg                                                                     >  1:7446929/1‑76 (MQ=255)

    gCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGc                                                                    <  1:3538512/76‑1 (MQ=255)

     cGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCt                                                                   >  2:1077993/1‑76 (MQ=255)

     cGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCt                                                                   >  2:8118148/1‑76 (MQ=255)

     cGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCt                                                                   >  2:6271053/1‑76 (MQ=255)

     cGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAAAGCt                                                                   >  1:208159/1‑76 (MQ=255)

      gTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCtt                                                                  >  1:1722857/1‑76 (MQ=255)

      gTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCtt                                                                  >  2:4342725/1‑76 (MQ=255)

       ttAAGCCGCACGGTTCAAAACGGCTGGGCACCCGAATGACGTCCGCGCCGCCCATAATGCCATGCGCCAAttcttc                                                                 >  2:2135985/1‑76 (MQ=255)

       ttAAGCCGCACGGTTCAAAACGGCTGGGCACCCGAATGACGTCCGAGCCGCCCATAATGCGATGCGCAAATGCTTc                                                                 >  2:6612542/1‑76 (MQ=255)

       ttAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTc                                                                 <  2:1737561/76‑1 (MQ=255)

        tAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGGTTCg                                                                >  1:92266/1‑76 (MQ=255)

        tAAGCCGCACGGTTCAAAACCGCTGGGTCACACCACGACGGGCCCGCCGCCCCTAATGCGAAGCGCAAAAGCTTCg                                                                >  2:1611795/1‑76 (MQ=255)

         aaGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGt                                                               <  2:5710919/76‑1 (MQ=255)

         aaGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGt                                                               >  2:2998115/1‑76 (MQ=255)

           gCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGa                                                             <  1:1831904/76‑1 (MQ=255)

           gCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCCATGCGCAAATGCTTCGGGa                                                             >  2:307289/1‑76 (MQ=255)

            ccGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGAt                                                            >  1:4691745/1‑76 (MQ=255)

            ccGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGAt                                                            <  1:768159/76‑1 (MQ=255)

            ccGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGAt                                                            >  1:1180643/1‑76 (MQ=255)

             cGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATa                                                           <  2:5114036/76‑1 (MQ=255)

             cGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATa                                                           <  2:6883617/76‑1 (MQ=255)

             cGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATa                                                           <  1:3478615/76‑1 (MQ=255)

              gCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAg                                                          >  1:1111046/1‑76 (MQ=255)

              gCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAg                                                          <  2:6072683/76‑1 (MQ=255)

               cACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGc                                                         <  2:322130/76‑1 (MQ=255)

                  ggTTCAAAACGGCTGGGCACCAGGATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCaa                                                      >  2:1948083/1‑76 (MQ=255)

                   gTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAAt                                                     >  2:5540869/1‑76 (MQ=255)

                   gTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAAt                                                     <  1:7967966/76‑1 (MQ=255)

                   gTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAAt                                                     <  1:5086760/76‑1 (MQ=255)

                   gTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAAt                                                     >  2:6684088/1‑76 (MQ=255)

                   gTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAAt                                                     >  1:4047634/1‑76 (MQ=255)

                    ttCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATc                                                    <  2:4098740/76‑1 (MQ=255)

                    ttCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATc                                                    <  2:409020/76‑1 (MQ=255)

                    ttCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATc                                                    <  1:2857773/76‑1 (MQ=255)

                     tCAAAACGGCTGGGCGACAGAAAGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCCATCt                                                   >  2:2891935/1‑76 (MQ=255)

                      cAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCCTAATGTGATGCGCAAATGATTCGTGATAGCCAATCTg                                                  >  2:7876093/1‑76 (MQ=255)

                      cAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTg                                                  <  1:7894906/76‑1 (MQ=255)

                      cAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTg                                                  <  1:302914/76‑1 (MQ=255)

                      cAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTg                                                  >  2:3472628/1‑76 (MQ=255)

                      cAAAACGGCTGGGCACCAGAATGACGCCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTg                                                  >  2:4951359/1‑76 (MQ=255)

                        aaaCGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTgta                                                >  2:1381778/1‑74 (MQ=255)

                        aaaCGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGaa                                                <  1:6044391/76‑1 (MQ=255)

                          aCGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATTTGAACg                                              >  1:6558390/1‑76 (MQ=255)

                          aCGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACg                                              <  2:5212352/76‑1 (MQ=255)

                            ggCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGcc                                            >  2:246623/1‑76 (MQ=255)

                             gCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGccc                                           <  1:5942453/76‑1 (MQ=255)

                             gCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGccc                                           <  1:6453282/76‑1 (MQ=255)

                              cTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCa                                          <  1:4732460/76‑1 (MQ=255)

                              cTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCa                                          >  1:7628010/1‑76 (MQ=255)

                              cTGGGCACCAGAATCACGTCCGCGCCGCCCATAATGCGAGGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCa                                          <  2:3152194/76‑1 (MQ=255)

                                gggCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCAcc                                        >  1:5302726/1‑76 (MQ=255)

                                 ggCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCAccc                                       >  2:3412813/1‑75 (MQ=255)

                                 ggCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCt                                       <  1:8192477/76‑1 (MQ=255)

                                  gCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTg                                      <  2:6045027/76‑1 (MQ=255)

                                  gCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTg                                      >  1:4227858/1‑76 (MQ=255)

                                   cACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGa                                     >  1:1009507/1‑76 (MQ=255)

                                   cACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGa                                     >  2:6770010/1‑76 (MQ=255)

                                   cACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGa                                     <  2:935682/76‑1 (MQ=255)

                                     ccAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGAcc                                   <  2:2721477/76‑1 (MQ=255)

                                     ccAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGAcc                                   <  2:7151036/76‑1 (MQ=255)

                                      cAGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCGCCCTGACCg                                  >  1:6196762/1‑76 (MQ=255)

                                       aGAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCgg                                 >  2:7421858/1‑76 (MQ=255)

                                        gAATGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCggg                                <  2:1065161/76‑1 (MQ=255)

                                          aTGACGTCCGCGCCGCCCATAATGCGATGCTCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGt                              <  2:4829107/76‑1 (MQ=255)

                                          aTGACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGt                              <  1:5818555/76‑1 (MQ=255)

                                            gACGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTAt                            >  2:780832/1‑76 (MQ=255)

                                             aCGTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTAtt                           >  2:6558546/1‑76 (MQ=255)

                                               gTCCGCGCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTcc                         <  1:6333565/76‑1 (MQ=255)

                                                  cgcgCCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCt                      >  1:4180367/1‑76 (MQ=255)

                                                   gcgcCGCCCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTg                     <  1:6847004/76‑1 (MQ=255)

                                                     gccgccCATAATGCTATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTgcc                   >  1:2280978/1‑76 (MQ=255)

                                                     gccgccCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTgcc                   <  1:7044216/76‑1 (MQ=255)

                                                        gccCATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTgccgcc                >  2:3176079/1‑76 (MQ=255)

                                                         cccATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGccgccg               <  2:2253415/76‑1 (MQ=255)

                                                           cATAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCa             >  2:3389587/1‑76 (MQ=255)

                                                             tAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAg           <  1:1048294/76‑1 (MQ=255)

                                                             tAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAg           >  1:7804440/1‑76 (MQ=255)

                                                             tAATGCGATGCGCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAg           >  2:1265288/1‑76 (MQ=255)

                                                              aaggcgaggcgaAAATGCTTCGTGATAGCCAATCTGAACGCCCCCCGGCCCGGGGTTTTCCGCTGCCGCCGCAAgg          <  2:38038/73‑1 (MQ=255)

                                                               attcgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGa         >  1:4387431/4‑76 (MQ=255)

                                                               atgcgatgcgCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGa         <  1:6663494/76‑1 (MQ=255)

                                                               atgcgatgcgCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGa         >  1:236078/1‑76 (MQ=255)

                                                               atgcgatgcgCAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGa         <  1:3227501/76‑1 (MQ=255)

                                                               atgcaaggcgCAAAGGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGa         <  1:2550206/76‑1 (MQ=255)

                                                                tgcgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGaa        >  2:371931/1‑76 (MQ=255)

                                                                tgcgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGaa        <  1:3585682/76‑1 (MQ=255)

                                                                tgcgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGaa        <  1:4546657/76‑1 (MQ=255)

                                                                 gcgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGaaa       >  2:4906146/1‑76 (MQ=255)

                                                                  cgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAAc      >  1:3775507/1‑76 (MQ=255)

                                                                  cgatgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAAc      >  2:2124543/1‑76 (MQ=255)

                                                                    atgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCt    >  2:5978862/1‑76 (MQ=255)

                                                                    atgcgcAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCt    >  1:7231511/1‑76 (MQ=255)

                                                                     tgcgaAAATGCTTCGTGATAGCCAATCTGACCGCCCCCCTGCCCGGGGTATTCCGCTGCCGCCGCAAGGAAACCtt   <  2:1872382/76‑1 (MQ=255)

AGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTC  >  REL606/3498337‑3498482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`