| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,570 | T→A | I572N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,570 | 0 | T | A | 98.9% | 1080.1 / 1.4 | 262 | I572N (ATC→AAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base A (128/131); total (131/131) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
Only 100 of 271 total aligned reads displayed.
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162497‑4162642 | cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACa < 2:5575536/76‑1 (MQ=255)cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACa > 2:5306832/1‑76 (MQ=255) gaggTACGCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAAct < 1:1540007/73‑1 (MQ=255) gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctc > 1:3159857/1‑76 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctct > 1:130113/1‑76 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctct > 2:2521708/1‑76 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTg < 2:2275720/76‑1 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTg > 1:7167576/1‑76 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTg > 1:3572894/1‑76 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTg > 1:939377/1‑76 (MQ=255) cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGt > 2:6998197/1‑76 (MQ=255) acCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTc > 1:7729265/1‑76 (MQ=255) ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCg < 2:2081265/76‑1 (MQ=255) ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCg > 2:5473779/1‑76 (MQ=255) ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCg < 2:48304/76‑1 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtg > 1:4217993/1‑76 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtg > 1:5783497/1‑76 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtg < 2:1842515/76‑1 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtg > 2:2882741/1‑76 (MQ=255) gcTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt < 2:5956537/75‑1 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt > 1:7416946/1‑76 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt > 2:409960/1‑76 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt > 2:1069910/1‑76 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTa > 1:6921925/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGca < 1:5058330/76‑1 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGca < 1:823345/76‑1 (MQ=255) tACGGTCGCGTATGCCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGcac < 1:5707565/76‑1 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGcaca > 2:5791199/1‑76 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGcaca < 1:6679773/76‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAg < 2:6004415/76‑1 (MQ=255) ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGa > 1:5873377/1‑76 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGAc > 1:544950/1‑76 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACt > 2:6329214/1‑76 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACt < 1:5609242/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa > 1:4454382/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa > 2:4429153/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa < 2:6845629/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa < 1:7911675/76‑1 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTaa < 1:6299661/76‑1 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTaa > 1:7958969/1‑76 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc > 2:370743/1‑76 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg > 1:5369245/1‑76 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg < 2:5712500/76‑1 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg < 2:900987/76‑1 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg < 2:499907/76‑1 (MQ=255) ctATGTCGAATCTAACACCTTGACGTTCCGAACAGCCGTCTAAACACTTCTCAGTCCGTGGACCCACAGACTAACg < 2:499908/75‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGaa < 2:6950066/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 1:1847477/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 1:6492485/76‑1 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt > 1:6074360/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 1:1200203/76‑1 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 1:2448327/76‑1 (MQ=255) attCCAATCGAAACCCCTGAAGGTCCGACCATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 2:753301/74‑1 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 2:8240877/76‑1 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 2:6792162/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 1:2997459/1‑76 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 1:1627318/1‑76 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:7671762/1‑76 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:392624/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:7358257/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:1238594/76‑1 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:2775406/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACACACTAACGAATACgg > 2:796068/1‑76 (MQ=255) aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 1:7106411/76‑1 (MQ=255) aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 1:3968926/76‑1 (MQ=255) tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:1074099/76‑1 (MQ=255) tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:7772756/76‑1 (MQ=255) cGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 1:2065588/1‑76 (MQ=255) gAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtttc > 2:3460027/1‑74 (MQ=255) gAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtttc > 1:2713182/1‑74 (MQ=255) acccccGGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:2599198/74‑1 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:3376252/76‑1 (MQ=255) cccTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:7251637/76‑1 (MQ=255) cTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTTAGAc > 2:6648747/1‑76 (MQ=255) tGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 1:3717910/76‑1 (MQ=255) tGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 2:4290615/76‑1 (MQ=255) tGAAGGTCCAAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt > 1:6158921/1‑76 (MQ=255) gAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTTCGACTc > 2:7793930/1‑76 (MQ=255) aaGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACttc > 2:7249803/1‑74 (MQ=255) aGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg < 2:3240418/76‑1 (MQ=255) ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTccct > 1:1678527/1‑74 (MQ=255) ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 1:3058636/76‑1 (MQ=255) gatCCGAACATCGGTCTGAACACGTCGCTGTCCGTGTACGCACAGCCTAACGAATACGGCTTCCTTGAGACTCCGt < 2:1679463/74‑1 (MQ=255) gTCCGAACATCGGTCTGAACAACTCTCTGTCTGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa < 2:2250634/76‑1 (MQ=255) gTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa > 1:1007379/1‑76 (MQ=255) tCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt < 2:1577635/76‑1 (MQ=255) ccGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCGTATc > 1:4051143/1‑76 (MQ=255) ccGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc > 1:3466724/1‑76 (MQ=255) cGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg > 1:8100237/1‑76 (MQ=255) gAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt < 1:1485993/76‑1 (MQ=255) aaCATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCGTATCGTa > 2:3018529/1‑76 (MQ=255) aaCATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa < 2:1431140/76‑1 (MQ=255) cATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa < 2:6209279/76‑1 (MQ=255) aTCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGGATCCGTATCGTaaaa > 2:4634965/1‑75 (MQ=255) ggTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 2:7102407/76‑1 (MQ=255) tCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCGTATCGTAAAgagaac > 2:3760832/1‑74 (MQ=255) tCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc < 1:4281859/76‑1 (MQ=255) tCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc < 2:3579540/76‑1 (MQ=255) cTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTTAGACTTCGTATCCTAAAATGACCg > 2:4536983/1‑76 (MQ=255) tGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAccca > 1:1597189/1‑74 (MQ=255) | CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162497‑4162642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |