| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,438,531 | T→G | D110A (GAC→GCC) | fbp ← | fructose‑1,6‑bisphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,438,531 | 0 | T | G | 99.2% | 1052.6 / ‑3.9 | 253 | D110A (GAC→GCC) | fbp | fructose‑1,6‑bisphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (101/150); total (101/152) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 257 total aligned reads displayed.
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATG > REL606/4438458‑4438605 | aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 1:6066458/76‑1 (MQ=255)aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 1:909959/76‑1 (MQ=255) aCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCa < 2:6142096/76‑1 (MQ=255) gggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa < 2:7326935/75‑1 (MQ=255) cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa < 2:3960937/76‑1 (MQ=255) ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg > 1:7502521/1‑76 (MQ=255) gcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGc < 1:8140137/76‑1 (MQ=255) ggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcac < 2:7933738/76‑1 (MQ=255) gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc > 2:8166160/1‑76 (MQ=255) gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc > 2:3314370/1‑76 (MQ=255) gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc > 2:2337774/1‑76 (MQ=255) tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 2:4937200/1‑76 (MQ=255) tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 1:4800097/1‑76 (MQ=255) aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac > 1:3934213/1‑76 (MQ=255) aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac < 2:2967103/76‑1 (MQ=255) aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac > 1:879125/1‑76 (MQ=255) aGATGGAGAAAAGGGTACCGACAGAGACGTTAACATCGATGTGGGACGAGCCATCCAGGGGGGCCATCAGcaccac < 2:341978/76‑1 (MQ=255) aTGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGt < 1:3273344/76‑1 (MQ=255) gagaAAATGTTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtt < 2:4288857/76‑1 (MQ=255) agaAAATGGTCCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAttt < 2:7755687/76‑1 (MQ=255) agaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAttt < 2:6368611/76‑1 (MQ=255) gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtttt < 2:1840025/76‑1 (MQ=255) aaaaTGGTCCCGACAGAGACGTTAACATCGATGTTGGAGGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg < 1:4382789/76‑1 (MQ=255) aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg > 2:1999692/1‑76 (MQ=255) aaaGGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGc < 1:1171253/76‑1 (MQ=255) aaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCg > 1:4605970/1‑76 (MQ=255) gTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGtt < 2:751719/76‑1 (MQ=255) cccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca < 2:110392/75‑1 (MQ=255) aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca < 2:6806713/76‑1 (MQ=255) aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca < 2:5690634/76‑1 (MQ=255) aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca > 2:4277058/1‑76 (MQ=255) caaCAGATACGTTAACTTGGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 1:6298079/74‑1 (MQ=255) cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 1:2338501/76‑1 (MQ=255) cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 1:7963101/76‑1 (MQ=255) gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAg < 1:1588806/76‑1 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc < 1:6909720/76‑1 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 1:1076003/1‑76 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc < 2:6090199/76‑1 (MQ=255) cAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGcc > 2:3668/1‑76 (MQ=255) agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 2:5313756/1‑76 (MQ=255) agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCACCAGCACCACGTATTTTGCGTGTTCACAGCCt > 1:3983216/1‑76 (MQ=255) gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt < 1:786058/76‑1 (MQ=255) gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt > 1:1697158/1‑76 (MQ=255) gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt > 1:2861777/1‑76 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:7328791/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:1028166/76‑1 (MQ=255) gtttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa < 1:3433911/74‑1 (MQ=255) cGTTAACATCGAGGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa < 1:1270883/76‑1 (MQ=255) gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg > 2:5955069/1‑76 (MQ=255) gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 1:2792180/76‑1 (MQ=255) ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga > 1:4876590/1‑76 (MQ=255) ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga > 2:1088144/1‑76 (MQ=255) tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 2:412736/76‑1 (MQ=255) tAACATCGATGTGGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 1:5175628/76‑1 (MQ=255) aaCATGGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg < 1:4646375/76‑1 (MQ=255) aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 1:8250354/1‑76 (MQ=255) aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 1:309516/1‑76 (MQ=255) aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 1:5975734/1‑76 (MQ=255) cATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacgac < 1:3493184/76‑1 (MQ=255) cATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacgac < 2:2596372/76‑1 (MQ=255) cATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacgac < 2:4673389/76‑1 (MQ=255) cATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacgac < 1:7047734/76‑1 (MQ=255) aTCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa > 1:1879883/1‑76 (MQ=255) aTCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa > 1:7215127/1‑76 (MQ=255) tCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACaa > 1:4166316/1‑76 (MQ=255) tCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACaa > 2:4758505/1‑76 (MQ=255) aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct > 1:2138911/1‑76 (MQ=255) tGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtctc < 1:7579758/76‑1 (MQ=255) gTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCa > 1:6860537/1‑76 (MQ=255) gTTGAACGAGCCATCCAGGGGGGCCATCAGCGCCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCa < 2:7395384/76‑1 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt > 2:3870527/1‑76 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAGCTCAt > 2:7423704/1‑76 (MQ=255) tgggaggaGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 2:2803332/74‑1 (MQ=255) tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 2:6001130/1‑76 (MQ=255) ggACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct > 1:6665539/1‑76 (MQ=255) ggACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct > 1:6270016/1‑76 (MQ=255) ggACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct > 1:7723036/1‑76 (MQ=255) ggACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct > 1:542657/1‑76 (MQ=255) ggACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct < 2:8209502/76‑1 (MQ=255) gccGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtctt < 2:5057681/74‑1 (MQ=255) cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct > 2:3742397/1‑76 (MQ=255) cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct > 2:6199036/1‑76 (MQ=255) aGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttc < 2:1293370/76‑1 (MQ=255) aGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttc < 2:7604162/76‑1 (MQ=255) aGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttc > 1:5749217/1‑76 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt > 1:4071261/1‑76 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 1:583972/76‑1 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 1:8082355/76‑1 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt > 2:1555199/1‑76 (MQ=255) tCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCag < 2:2268052/76‑1 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 2:6586907/76‑1 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag > 1:1411874/1‑76 (MQ=255) aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg > 1:5112457/1‑76 (MQ=255) aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg > 2:1961031/1‑76 (MQ=255) aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg < 2:886804/76‑1 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa > 2:7113200/1‑76 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa > 1:7408281/1‑76 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:240365/76‑1 (MQ=255) ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa < 1:2972345/76‑1 (MQ=255) gTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATg < 2:5491770/76‑1 (MQ=255) | AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATG > REL606/4438458‑4438605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |