| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,668 | C→T | W213* (TGG→TAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,668 | 0 | C | T | 97.3% | 676.8 / 5.0 | 185 | W213* (TGG→TAG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (102/78); total (104/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01 | |||||||||||
Only 100 of 219 total aligned reads displayed.
CCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCA > REL606/474595‑474740 | cccTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTAc > 1:6546096/1‑76 (MQ=255) cTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACgg < 1:4168722/76‑1 (MQ=255) tAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGc < 1:6484663/76‑1 (MQ=255) aaCGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCa > 1:720704/1‑76 (MQ=255) aaCGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGCTGCCGCGCTCCGTGCCATAATCTACGGCa > 1:8015777/1‑76 (MQ=255) aCGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCAc < 1:5088331/76‑1 (MQ=255) cgccagcgccagCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGGGCTGAGTGCCATAATCTACGGCAcc > 2:750128/1‑76 (MQ=255) gccagcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCa > 1:4322894/1‑76 (MQ=255) ccagcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAg < 1:6310338/76‑1 (MQ=255) ccagcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAg > 1:4033154/1‑76 (MQ=255) ccagcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAg < 2:2484820/76‑1 (MQ=255) cagcccctgcTCCGACAGGGTAAACAGCTGGCGAGAACCGATTGCCGAGCTGCGTGCCATAATCTCCGGCACCAGa < 2:4818902/76‑1 (MQ=255) agcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGAc > 1:6444511/1‑76 (MQ=255) gcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACg < 2:2204957/76‑1 (MQ=255) cgccagcACCGACAGGGTAAACAGCTCGCGGGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACgg < 2:4422253/76‑1 (MQ=255) cgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTGCGGGACCAGACgg > 2:7536469/1‑76 (MQ=255) cgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACgg > 2:5954170/1‑76 (MQ=255) cgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACgg > 1:8190187/1‑76 (MQ=255) cgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACgg > 1:4884352/1‑76 (MQ=255) ccagcCCCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCg < 1:6736047/76‑1 (MQ=255) cACCGACAGGGTAAACAGCTCGCGGGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCt < 1:5719911/76‑1 (MQ=255) cACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCt > 1:1063250/1‑76 (MQ=255) cACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCt > 2:6118469/1‑76 (MQ=255) cACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCt > 2:411956/1‑76 (MQ=255) cGACAGGTGAAACAGCCCGCGAGAACCGGTTGCCGCCCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTAcc < 1:702392/76‑1 (MQ=255) cGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGAGACCTAcc > 2:1384833/1‑76 (MQ=255) gACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCa < 2:2896149/76‑1 (MQ=255) cAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGc < 1:7889937/76‑1 (MQ=255) cAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGc > 1:6131218/1‑76 (MQ=255) aaGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGc < 2:7946647/75‑1 (MQ=255) aGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGca > 2:5411344/1‑76 (MQ=255) aGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGca > 1:1456268/1‑76 (MQ=255) gggTAAACAGCTCGCGAGACCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGcat < 1:6947525/76‑1 (MQ=255) gggTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGcat < 1:7446347/76‑1 (MQ=255) gTAAACGGCTCGCGAGAACCGTTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGcatca < 2:3283578/76‑1 (MQ=255) gTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGcaaca > 2:2856607/1‑76 (MQ=255) gTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatca > 1:1269068/1‑76 (MQ=255) tAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGcatcat > 1:4174412/1‑76 (MQ=255) aaaCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATa < 1:2034467/76‑1 (MQ=255) aaaCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATa > 1:3920850/1‑76 (MQ=255) aaaCAGCCCGGAAGAACCGTTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATa < 1:700245/76‑1 (MQ=255) aaaCACCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATa < 2:920799/76‑1 (MQ=255) aaCGGCTCGCGGGACCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCCCCAGACGGCGACCTACCAGCATCATaa < 2:4692560/76‑1 (MQ=255) aaCAGCTCGCGAGACCGGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATaa < 2:5125612/76‑1 (MQ=255) aaCAGCTCGCGAGACCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATaa < 2:3178111/76‑1 (MQ=255) aaaGCACGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAAt < 2:6538240/74‑1 (MQ=255) aCAGCTCGCGAGAACCGTTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAAt < 1:5324197/76‑1 (MQ=255) aCAGCTCGCGAGAACCGGTTGCCTCGTGGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAAt < 2:6158530/76‑1 (MQ=255) aCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAAt < 1:5234578/76‑1 (MQ=255) cAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATg > 2:1800388/1‑76 (MQ=255) cAGCGCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATg < 2:1019881/76‑1 (MQ=255) gcgcgcgAAATCCGGTTGCCGCGCGGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGc < 2:1238575/73‑1 (MQ=255) cgcgcgGGAACCGGTTGCCGCGCTGCGGGCCATAAACTCCGGCACCAGACGGCGACCTACCAGCATCATAATGGCg < 2:5109160/74‑1 (MQ=255) cccgcgAGAACCGGTGGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCg < 2:1601186/74‑1 (MQ=255) cgcgAGACCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGAt < 2:6854196/76‑1 (MQ=255) cgcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGAt > 2:7335457/1‑76 (MQ=255) cgcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGAt > 1:1829253/1‑76 (MQ=255) cgcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGAt > 1:1751572/1‑76 (MQ=255) gcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATa > 2:7188471/1‑76 (MQ=255) ccaagaCCCGGTTGCCGCGTTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATa < 1:261356/73‑1 (MQ=255) cgAGAACGGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATaa < 1:1158664/76‑1 (MQ=255) cgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATaa > 1:3610606/1‑76 (MQ=255) cgAGAACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATaa > 2:1172832/1‑76 (MQ=255) gagaACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATaaa < 2:5932461/76‑1 (MQ=255) gagaACCGGTTGCCGCGCTGCGCGCCATAATCTACGGCACCAGACGGCGCCCTACCAGCATCATAATGGCGATaaa > 2:7917391/1‑76 (MQ=255) gaaaaCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATaaa < 2:7777777/73‑1 (MQ=255) agaACCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAAt > 1:7308059/1‑76 (MQ=255) aaCCGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATCGCGATAAATGc > 2:2330691/1‑76 (MQ=255) ccGGTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGa > 2:5368085/1‑76 (MQ=255) cGGTTGCCGCGCTGCGTGCCATAATCTACGGCCCCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGAt < 1:5156255/76‑1 (MQ=255) cGGTTGCCGCGCTGCGGGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGAt < 2:1987021/76‑1 (MQ=255) gtttGCCGCGCGGCGGGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATc < 1:3116464/74‑1 (MQ=255) ggTTGCCGCGCTGCGTCCCATAATCTACGGCCCCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATc < 1:573650/76‑1 (MQ=255) gTTGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCa < 2:8053192/76‑1 (MQ=255) gTTGCCGCCCTGAGTCCCATAATCTACGACACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCa < 2:4317101/76‑1 (MQ=255) ttGCCGCGCTGCGTGCCATAATCTCCGGCCCCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCAc < 1:924444/76‑1 (MQ=255) ttGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCAc < 2:740342/76‑1 (MQ=255) tGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACt > 1:3176631/1‑76 (MQ=255) tGCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACt > 1:4369903/1‑76 (MQ=255) gCCGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACtt < 1:3639538/76‑1 (MQ=255) cccGCGCGGGGTGCCATATTCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACtt < 2:6754818/75‑1 (MQ=255) ccGCGCTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACttt < 2:3672328/76‑1 (MQ=255) gcgcTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGc > 2:6022748/1‑76 (MQ=255) gcTGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCg > 2:1949368/1‑76 (MQ=255) tGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGAt > 1:5544788/1‑76 (MQ=255) tGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGAt > 2:3960428/1‑76 (MQ=255) tGCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGAt > 2:4335699/1‑76 (MQ=255) gCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATg > 1:4469378/1‑76 (MQ=255) gCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATg > 2:2262100/1‑76 (MQ=255) gCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATg > 2:2687110/1‑76 (MQ=255) gCGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATg > 2:5290243/1‑76 (MQ=255) cGTGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATgg > 2:725631/1‑76 (MQ=255) tGCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTg > 1:3676979/1‑76 (MQ=255) gCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa > 2:5665018/1‑76 (MQ=255) gCCATAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGa < 1:5829451/76‑1 (MQ=255) aTAATCTACGGCACCAGACGGGGACCTCCCAGCATCATAACGGCGATAAATGCGAAAACTTTGCCGACGGTGATcc > 2:8219444/1‑76 (MQ=255) aTAATCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATcc > 1:234571/1‑76 (MQ=255) aaTCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATcccc > 1:3810325/1‑76 (MQ=255) aaTCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATAGTGATcccc < 2:3920850/76‑1 (MQ=255) aTCTACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCa < 2:1260032/76‑1 (MQ=255) | CCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCA > REL606/474595‑474740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |