| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 650,624 | T→G | K60Q (AAG→CAG) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 650,624 | 0 | T | G | 97.2% | 871.0 / 4.6 | 211 | K60Q (AAG→CAG) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (65/140); total (68/143) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 233 total aligned reads displayed.
GTGCGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGAT > REL606/650551‑650696 | gCGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATg > 1:1449537/1‑76 (MQ=255) gCGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATg < 2:653703/76‑1 (MQ=255) cGGTTGAGTGCCAGAGGGATCCCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGc < 2:736513/76‑1 (MQ=255) cGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGGGCGATAGGCACCCGCTGAATgg > 2:6457004/1‑75 (MQ=255) cGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGc < 2:7981347/76‑1 (MQ=255) cGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGc < 2:2327018/76‑1 (MQ=255) cGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGc < 2:337891/76‑1 (MQ=255) ggTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCg > 1:3742045/1‑76 (MQ=255) gTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCgg > 1:5054916/1‑76 (MQ=255) gTTGAGTGCAAGAGGGATACCGTTACGTTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCgg < 2:3182308/76‑1 (MQ=255) tGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGGTGAATGCgggt > 2:2159749/1‑74 (MQ=255) gTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGGTTTc > 2:1304651/1‑76 (MQ=255) tGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCa < 2:3124288/76‑1 (MQ=255) tGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCa < 2:4180184/76‑1 (MQ=255) gCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCCGCTGAATGCGGGTTTcct > 1:7578463/1‑74 (MQ=255) gCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCAt < 1:8165229/76‑1 (MQ=255) gCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGGTTTCAt > 1:6527800/1‑76 (MQ=255) gCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCCATAAGCACCAGCTGCATGCGGGTTTCAt > 2:253800/1‑76 (MQ=255) ccAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCAtt < 2:580306/76‑1 (MQ=255) ccAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCAtt > 1:1086337/1‑76 (MQ=255) cAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCCGCTGAATGCGGGTTTCATTa > 1:3340059/1‑76 (MQ=255) cAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTa < 2:3922479/76‑1 (MQ=255) cAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTa < 2:2955088/76‑1 (MQ=255) agagGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTag < 2:4419591/76‑1 (MQ=255) agagGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTag < 2:1408094/76‑1 (MQ=255) gagGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTaga < 2:355384/76‑1 (MQ=255) gagGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTaga < 2:7150978/76‑1 (MQ=255) atggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTagag < 2:2242599/74‑1 (MQ=255) agGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTagag < 2:515290/76‑1 (MQ=255) agGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTagag > 1:3697974/1‑76 (MQ=255) gggATGCCGTTACGGTCATGGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGc < 2:5669895/76‑1 (MQ=255) gggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGc < 1:4020893/76‑1 (MQ=255) gggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGc < 1:3009775/76‑1 (MQ=255) gggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGc < 1:3535471/76‑1 (MQ=255) ggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCg < 1:5683626/76‑1 (MQ=255) ggATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCg < 1:5658416/76‑1 (MQ=255) gATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCgg < 2:2785738/76‑1 (MQ=255) gATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCgg < 1:1532787/76‑1 (MQ=255) aTACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCggg > 2:7653353/1‑76 (MQ=255) aTACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCggg > 2:5545587/1‑76 (MQ=255) tACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGt > 1:4000335/1‑76 (MQ=255) tACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGGTTTCATTAGAGCGGGt > 1:7072581/1‑76 (MQ=255) ccGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCt < 1:3262667/76‑1 (MQ=255) gTTACGGTCATGGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTgg < 1:4361876/76‑1 (MQ=255) gTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTgg < 2:5945272/76‑1 (MQ=255) gTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTgg > 2:1004880/1‑76 (MQ=255) ttACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGt < 1:7343202/76‑1 (MQ=255) ttACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGt > 2:1662581/1‑76 (MQ=255) aCGGTCATAGATAATGCCGCGGCTGGGGGCGATAGGGACCAGCTGAATGCGGTTTTTATTAGAGCGGGTCTGGttg > 1:6685187/1‑74 (MQ=255) cGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGt < 1:8084434/76‑1 (MQ=255) gTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTCGAGCGGGTCTGGTAGTCg > 2:6674185/1‑76 (MQ=255) gTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCg < 1:3177114/76‑1 (MQ=255) cATAGATAATGCCGCGGCTGGGCGCGATAGGGACCAGCTGAATGCGGGTTTCATTAGCGCGGGTCTGGTAGTCCGt > 2:2630575/1‑76 (MQ=255) cATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGt > 2:7511819/1‑76 (MQ=255) cATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGt < 1:2555157/76‑1 (MQ=255) cATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGt < 1:4710082/76‑1 (MQ=255) cATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGt < 2:3656444/76‑1 (MQ=255) aTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTa < 1:2622580/76‑1 (MQ=255) tAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTaa < 2:3628276/76‑1 (MQ=255) tAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTaa < 1:7359604/76‑1 (MQ=255) tAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTaa < 2:4881505/76‑1 (MQ=255) tAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGGaa > 2:4798245/1‑76 (MQ=255) aGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTaaa < 1:6192180/76‑1 (MQ=255) aGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTaaa > 1:2485091/1‑76 (MQ=255) gATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAg > 2:3955452/1‑76 (MQ=255) gATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAg < 2:3192230/76‑1 (MQ=255) gATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAg > 1:2870857/1‑76 (MQ=255) gATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAg < 2:1758370/76‑1 (MQ=255) aTAATGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGc < 1:6941415/76‑1 (MQ=255) aaTGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCAATAGAGCGGGTCTGGTAGTCGGTAAAGCGa < 1:2069708/76‑1 (MQ=255) aTGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGaa > 1:4114066/1‑76 (MQ=255) aTGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGaa < 1:439296/76‑1 (MQ=255) tGCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAAc < 1:7018141/76‑1 (MQ=255) gCCGCGGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACa < 2:6183006/76‑1 (MQ=255) gcgGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAAtt < 2:3967136/76‑1 (MQ=255) cgGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAAttt > 1:8001714/1‑76 (MQ=255) cgGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAAttt < 2:789601/76‑1 (MQ=255) cgGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAAttt < 2:3101235/76‑1 (MQ=255) cgGCTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGGAAAGCGAACAAttt > 1:5342099/1‑76 (MQ=255) ggCTGTGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTg < 2:1770001/76‑1 (MQ=255) cTGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCa > 1:1035328/1‑76 (MQ=255) tGGGCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAg < 1:1880718/76‑1 (MQ=255) gggCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGa > 1:5937111/1‑76 (MQ=255) gggCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGa > 2:5355882/1‑76 (MQ=255) ggCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGAt > 2:4740220/1‑76 (MQ=255) ggCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGAt < 2:7306200/76‑1 (MQ=255) ggCGCGATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGAt < 1:7753343/76‑1 (MQ=255) cgcgATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATta < 1:7909816/76‑1 (MQ=255) gcgATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATtat < 1:792693/76‑1 (MQ=255) cgATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATtata < 2:4259437/76‑1 (MQ=255) gATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATAc > 1:6084511/1‑76 (MQ=255) gATAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATAc > 2:6981423/1‑76 (MQ=255) aTAGGCCCCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACa < 1:2752950/76‑1 (MQ=255) aTAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACa < 1:5611514/76‑1 (MQ=255) tAGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAg < 2:7817754/76‑1 (MQ=255) aGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAgg < 1:2190306/76‑1 (MQ=255) aGGCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAgg < 2:8000120/76‑1 (MQ=255) ggCACCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGt < 1:6367987/76‑1 (MQ=255) aCCAGCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTgg < 2:6767285/76‑1 (MQ=255) gCTGAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGAt < 1:5119297/76‑1 (MQ=255) | GTGCGGTTGAGTGCCAGAGGGATACCGTTACGGTCATAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGAT > REL606/650551‑650696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |