BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	506.4 / NA	127	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (61/66); total (61/66)

Only 100 of 159 total aligned reads displayed.

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        >  1:4268529/1‑76 (MQ=255)

 gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca                                                                       <  2:7280637/76‑1 (MQ=255)

 gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca                                                                       <  2:3742883/76‑1 (MQ=255)

  aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      >  2:3506419/1‑76 (MQ=255)

  aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      <  1:2991227/76‑1 (MQ=255)

  aGTCCCCGGCAAAAGACAAAACGATCAACAAATATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      <  2:4112576/76‑1 (MQ=255)

   gTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATc                                                                     <  1:5694648/76‑1 (MQ=255)

   gTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATc                                                                     >  1:5836127/1‑76 (MQ=255)

    tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  1:4736707/1‑76 (MQ=255)

    tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    <  2:6514150/76‑1 (MQ=255)

     ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   <  2:2792227/76‑1 (MQ=255)

       ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  1:3468002/76‑1 (MQ=255)

       ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  2:8129436/76‑1 (MQ=255)

         ggCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAt                                                               <  1:4905433/76‑1 (MQ=255)

           cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:4442309/76‑1 (MQ=255)

           cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  1:5708140/76‑1 (MQ=255)

           cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  2:1005751/76‑1 (MQ=255)

           cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt                                                             <  1:4980051/76‑1 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            <  2:8240403/76‑1 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            <  2:1417132/76‑1 (MQ=255)

             aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  1:6562251/1‑76 (MQ=255)

             aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGGTACATCCGCGATTTGc                                                           >  1:682870/1‑76 (MQ=255)

              aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  2:6736092/1‑76 (MQ=255)

               aGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCg                                                         <  2:2641204/76‑1 (MQ=255)

                 ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAc                                                       <  1:2711206/76‑1 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  1:3848232/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  2:388141/1‑76 (MQ=255)

                   aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     >  2:7724742/1‑76 (MQ=255)

                   aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     <  2:1744391/76‑1 (MQ=255)

                       cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGtcg                                                 >  2:1589068/1‑74 (MQ=255)

                       cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTgg                                                 >  2:2218544/1‑76 (MQ=255)

                       cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGAgg                                                 >  1:3698004/1‑76 (MQ=255)

                        gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                <  1:2979249/76‑1 (MQ=255)

                         aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               >  2:2364704/1‑76 (MQ=255)

                          tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATTCGCGATTTGCCGACCAGTGGCTc                                              >  2:7780252/1‑76 (MQ=255)

                          tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              >  1:2228094/1‑76 (MQ=255)

                          gcaacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              <  2:3637723/75‑1 (MQ=255)

                           caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                             <  1:6770250/76‑1 (MQ=255)

                           caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                             <  1:8207165/76‑1 (MQ=255)

                            aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            >  2:8162578/1‑76 (MQ=255)

                            aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            <  1:5335652/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:6191537/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:6399463/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:7488319/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:8064574/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:4505142/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:1160718/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          >  1:4084690/1‑76 (MQ=255)

                              caaGTATCGGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:1327588/76‑1 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         <  2:802857/76‑1 (MQ=255)

                                aGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGc                                        <  1:3750293/76‑1 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGccc                                      >  1:6757724/1‑75 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCATCTGccca                                     >  2:6527732/1‑74 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     >  2:5217233/1‑76 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     >  2:6158531/1‑76 (MQ=255)

                                    tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaa                                    <  1:6466479/76‑1 (MQ=255)

                                    tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaa                                    >  2:3060500/1‑76 (MQ=255)

                                     cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAACTGCCaaaa                                   >  2:2255004/1‑76 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  <  1:824580/76‑1 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  <  1:6320079/76‑1 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  <  1:1284090/76‑1 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAACTGCCcaaaa                                  >  2:4569283/1‑76 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 <  1:5035027/76‑1 (MQ=255)

                                         gTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagag                               >  1:7089263/1‑76 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagagg                              >  2:581303/1‑75 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              >  1:3946643/1‑76 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              >  1:7207803/1‑76 (MQ=255)

                                           aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  1:1027317/1‑76 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:4046430/76‑1 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  1:3668394/76‑1 (MQ=255)

                                               aCTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGa                         <  1:11129/76‑1 (MQ=255)

                                                cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGGCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  1:2165432/1‑76 (MQ=255)

                                                cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  2:243194/1‑76 (MQ=255)

                                                 tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                       >  2:632250/1‑76 (MQ=255)

                                                  aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      >  2:7546669/1‑76 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  1:7327995/76‑1 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  1:2030556/76‑1 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     >  2:7743998/1‑76 (MQ=255)

                                                    tgggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:2951454/74‑1 (MQ=255)

                                                    gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    >  1:766965/1‑76 (MQ=255)

                                                    gtggtaAAATCCAGCGACGGTTACAGCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:7197869/76‑1 (MQ=255)

                                                     tggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCCGCTGCCAAAAAGAGTGCCGACTCTAc                   >  1:6428867/1‑76 (MQ=255)

                                                     tggtgAAACCCTGCGTCGTTTACATCCGCGTTTTGCCGACCAGTGGCCCAGCTGCCAAAAAGAGTGCCGACTCTAc                   <  2:6924709/76‑1 (MQ=255)

                                                        tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc                >  2:5236849/1‑76 (MQ=255)

                                                        gaaaaTCCACGTTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc                <  2:5673855/74‑1 (MQ=255)

                                                          aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcca              >  2:3309932/1‑76 (MQ=255)

                                                            acccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:1011247/74‑1 (MQ=255)

                                                            aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:3440706/76‑1 (MQ=255)

                                                             tCCAGGGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca           <  2:397455/76‑1 (MQ=255)

                                                              cccgCGTCGGTTACATCCCCGATTTCCCGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  2:4268529/73‑1 (MQ=255)

                                                              ccAGCGTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  1:2359250/76‑1 (MQ=255)

                                                              ccAGCGTCGGTTCCATCCGCGTTTTGCCGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  2:5402740/76‑1 (MQ=255)

                                                              ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  1:3194321/76‑1 (MQ=255)

                                                               cAGCGTGGTTTACATCCGCGATTTGCGGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  2:3477997/76‑1 (MQ=255)

                                                               cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         >  2:4864755/1‑76 (MQ=255)

                                                               cAGCGTCGGTAACATCGGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  2:7630831/76‑1 (MQ=255)

                                                                   gTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACgg     <  1:3345687/76‑1 (MQ=255)

                                                                   gTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACgg     >  2:6906452/1‑76 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  1:2055495/76‑1 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGGGGCTCAGCTGCCAAAAAGAGTGGCGACTCTACCTCCAACAAGACGGCt   >  1:2705326/1‑76 (MQ=255)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`