| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.4% | 637.5 / NA | 169 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (53/114); total (53/116) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 185 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCC‑GGAAACAAACCCAATAAAAGCAAAACGCCCATcccccc > 2:6880560/1‑74 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:6350169/76‑1 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCG‑GGAAACAACCCCCATAAAACCAAAACGCCAAACCccacca > 1:2196911/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:5235043/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa < 1:4104387/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GAAATCAGCCCCCATAATAGCAAAACGACAAACCCCCACAGa > 1:855107/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GGAATCAGCCCCAATAACAGCAAAAAGCCCAACCCCCCCAGaa > 1:489615/1‑75 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 1:4507951/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:7968055/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGGCG‑GAAATCCGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 2:8278792/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:1164627/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 2:1566924/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGGTTTCGCGAAACCA‑CCCCAATAACAAACAAACACCCAACCCCACCCCATaaa > 2:1661844/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGGCGTGG‑GGAAAAAACCCCCAAAACAGCAAAACACCAATCCCCCCCCGAaaaa > 2:6491266/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:5580542/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:6619311/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:2075476/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGCTGTCG‑GGAAACAGCCCCAATAAAAGCCAAACGCCCAATCCCCCCCGATAAAGc > 1:5467741/1‑76 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 2:4636940/76‑1 (MQ=255) gTCCAGGCGCGGCTGGGCTGGCTGTTG‑GAAAACAACCCCAATAACAGCAAAAAACCAAATCCCACCACATAAAACa > 2:7023067/1‑76 (MQ=255) ccAGGCGCGGCTGGGCTGGGTGTCG‑GAAAATAGCCCCCATAAAAGCAAAACACCAATCCCCACCCGAAAAAACATg > 2:6043154/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:6229406/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:4363936/76‑1 (MQ=255) aGGCGCGGCTGGGCTGGCTGGCG‑GAAAACAGCCCCCAAAAAAGCAAAAAGCCCAACCCCCCCCGATAAAACATGAc > 2:3853594/1‑76 (MQ=255) gcgGGTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:7518362/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:4424714/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:7031581/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:2898250/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:328285/76‑1 (MQ=255) ggCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 1:1215576/1‑76 (MQ=255) ggCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:1613468/76‑1 (MQ=255) gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 1:7007511/76‑1 (MQ=255) gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 1:7058866/1‑76 (MQ=255) gCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 2:1999603/76‑1 (MQ=255) cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACCGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGAAt > 2:6184953/1‑76 (MQ=255) cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTatat > 2:4041302/1‑76 (MQ=255) cTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:832575/76‑1 (MQ=255) tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:7271186/76‑1 (MQ=255) tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:7826567/1‑76 (MQ=255) tGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACACCTGTAtt > 2:630094/1‑76 (MQ=255) ggTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:2008035/1‑76 (MQ=255) ggTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:739679/1‑76 (MQ=255) gTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:5637192/76‑1 (MQ=255) gTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:3668411/76‑1 (MQ=255) cTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 2:202673/76‑1 (MQ=255) cTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:2475740/76‑1 (MQ=255) tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 1:2158861/1‑76 (MQ=255) tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:4441980/1‑76 (MQ=255) tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 1:8155828/76‑1 (MQ=255) tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:4536122/76‑1 (MQ=255) tGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:3400581/1‑76 (MQ=255) gCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 2:536845/76‑1 (MQ=255) cTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 1:5424106/1‑76 (MQ=255) tGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt < 1:6547197/76‑1 (MQ=255) gccG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 1:6436506/74‑1 (MQ=255) gTCG‑GAAATCAGCCCCAATAACGGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 2:734228/1‑76 (MQ=255) tCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt > 1:2524471/1‑76 (MQ=255) cG‑GAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 2:5230574/1‑76 (MQ=255) g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:8139048/76‑1 (MQ=255) g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 1:6717087/1‑76 (MQ=255) g‑gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:4231930/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:7842139/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:4244873/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:3402143/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:6300797/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:3630707/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 1:3294872/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:5536743/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:81030/1‑76 (MQ=255) aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc > 1:6103100/1‑76 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:4239938/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 1:4178431/1‑76 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 2:881514/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 1:5392424/1‑76 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 1:1067489/1‑76 (MQ=255) ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc < 1:2652371/76‑1 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:2798931/1‑76 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 2:7365234/76‑1 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 2:4808743/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:2794447/1‑76 (MQ=255) cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc < 1:7590014/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCTCATCGCGCAc > 2:6778178/1‑76 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc > 1:1673653/1‑76 (MQ=255) taaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc < 2:1133735/75‑1 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:3701123/1‑76 (MQ=255) cAAAACGCAAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:5219015/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:6537430/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:2411256/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:2643039/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:6837256/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 2:6206782/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 1:1444374/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 2:775213/76‑1 (MQ=255) cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:1367455/1‑76 (MQ=255) cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:1230221/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:1588580/1‑76 (MQ=255) ccAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTCCCGATGGCGCATCGCGCACCAgccgcc < 2:489615/76‑1 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 2:6863076/1‑76 (MQ=255) cAATCCCCACCAAATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca < 1:4977025/76‑1 (MQ=255) cAACCCCCACCAAATAAAGCATGACGCCGTTATAAAACAGGCTTTTTACCGATGGCGCATCGCGCCCCAGCCGCca < 2:2531380/76‑1 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCG‑GAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |