| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,147,762 | G→A | intergenic (+57/+22) | rpoD → / ← ygjF | RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,147,762 | 0 | G | A | 99.6% | 856.2 / NA | 224 | intergenic (+57/+22) | rpoD/ygjF | RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (124/99); total (124/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 248 total aligned reads displayed.
GCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAAC > REL606/3147689‑3147834 | cTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCa > 1:7581651/1‑76 (MQ=255) cTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCa > 1:1595415/1‑76 (MQ=255) cTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCa > 2:7937989/1‑76 (MQ=255) cTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCa > 2:1884449/1‑76 (MQ=255) cTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCa < 2:2382372/76‑1 (MQ=255) ccTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCt > 1:1345059/1‑76 (MQ=255) ccTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCt < 1:5609141/76‑1 (MQ=255) ccTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCt < 1:351655/76‑1 (MQ=255) cTGGACGATTAATGGGTGGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCtt < 2:7608406/76‑1 (MQ=38) cTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCtt < 2:598227/76‑1 (MQ=255) cTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCtt < 2:554836/76‑1 (MQ=255) ggACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCtttt < 1:5797369/76‑1 (MQ=255) ggACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCtttt < 2:3295889/76‑1 (MQ=255) aCGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCg > 2:3616563/1‑76 (MQ=255) aCGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCg < 1:812799/76‑1 (MQ=255) gATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGt < 2:4330651/76‑1 (MQ=255) gATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGt < 1:2566486/76‑1 (MQ=255) aTTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTg > 2:1987785/1‑76 (MQ=255) aTTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTg < 2:2195573/76‑1 (MQ=255) aTTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTg < 1:2216077/76‑1 (MQ=255) ttAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTgg > 2:6265684/1‑76 (MQ=255) aaTCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCg > 2:6870347/1‑76 (MQ=255) aaTCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCg > 2:129823/1‑76 (MQ=255) aaTCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCg > 2:2234818/1‑76 (MQ=255) aaTCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAAGCCCTCTGCACAAACACCACCTTTTCGGTGGCg > 2:6757966/1‑76 (MQ=255) aTCGGTAGGCCGGATGAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGt < 1:5384646/76‑1 (MQ=255) tCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCATTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtt < 1:6977498/76‑1 (MQ=255) tCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtt < 1:6126972/76‑1 (MQ=255) cGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGttt > 2:8042164/1‑76 (MQ=255) ggTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtttt > 2:4028580/1‑76 (MQ=255) gTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGttttt < 2:4454702/76‑1 (MQ=255) tAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtttttt > 2:507959/1‑76 (MQ=255) tAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtttttt > 2:4129279/1‑76 (MQ=255) tAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGtttttt < 1:7116309/76‑1 (MQ=255) aGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGTCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTa > 1:3960016/1‑76 (MQ=255) aGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTa < 2:2746957/76‑1 (MQ=255) ggCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTAt < 2:2424633/76‑1 (MQ=255) gCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATc > 2:2754724/1‑76 (MQ=255) ccGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCg < 2:3550955/76‑1 (MQ=255) ccGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCg > 1:4133828/1‑76 (MQ=255) cGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGc > 1:1188641/1‑76 (MQ=255) cGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGc > 2:1627794/1‑76 (MQ=255) cGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGc > 1:3183480/1‑76 (MQ=255) tCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCAc > 1:989422/1‑76 (MQ=255) cAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACg < 2:4515612/76‑1 (MQ=255) cAGGCGTTAACCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGAGGCGTTCTTTACCGACCAcc > 2:663992/1‑75 (MQ=255) aGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGc > 2:82057/1‑76 (MQ=255) aGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGc > 2:782302/1‑76 (MQ=255) gCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCAc < 1:2099638/76‑1 (MQ=255) gCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCAc > 1:6042070/1‑76 (MQ=255) cGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACt > 2:495875/1‑76 (MQ=255) cGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACt < 2:2903050/76‑1 (MQ=255) cGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACt > 1:676350/1‑76 (MQ=255) gTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTa < 1:4001703/76‑1 (MQ=255) gTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTa > 2:7016821/1‑76 (MQ=255) gTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTa > 1:5855929/1‑76 (MQ=255) ttACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAc < 2:8621/76‑1 (MQ=255) ttACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAc < 2:2898624/76‑1 (MQ=255) ttACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAc > 1:7085755/1‑76 (MQ=255) tACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAcc > 1:2398066/1‑76 (MQ=255) tACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAcc < 2:7253110/76‑1 (MQ=255) tACGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAcc > 2:4348115/1‑76 (MQ=255) tACGCCGCACCCGGCACTAGGCCCCCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTAcc < 1:1090028/76‑1 (MQ=255) aCGCCGCACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCa < 2:183708/76‑1 (MQ=255) cgccgcACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAg < 2:1687198/76‑1 (MQ=255) gcACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGcc > 1:7936678/1‑76 (MQ=255) cACCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCt < 1:6287924/76‑1 (MQ=255) ccccGGCACTAGGCCCTCTGCACAAACACCCCCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTg < 1:6298445/75‑1 (MQ=255) aCCCGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTg < 1:5000092/76‑1 (MQ=255) cccGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTgg < 2:1535161/76‑1 (MQ=255) ccGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGt > 2:2141876/1‑76 (MQ=255) ccGGCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGt > 1:5576188/1‑76 (MQ=255) ggCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTcc < 1:475723/76‑1 (MQ=255) gCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCa < 2:3730918/76‑1 (MQ=255) gCACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCa > 2:1069941/1‑76 (MQ=255) cACTAGTCCCTCTGCCAAACCGCCTCCCTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAg < 2:4748127/76‑1 (MQ=255) cACTAGGCCCTCTGCACAAGCACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAg < 1:2007961/76‑1 (MQ=255) cACTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCTCCTGGTCCAg < 2:4617474/76‑1 (MQ=255) aCTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGc > 1:8065097/1‑76 (MQ=255) aCTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGc < 1:5499530/76‑1 (MQ=255) cTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCt > 1:7187680/1‑76 (MQ=255) cTAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCt > 2:6431245/1‑76 (MQ=255) tAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTc < 1:3647024/76‑1 (MQ=255) tAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTc > 1:87670/1‑76 (MQ=255) tAGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTc > 1:5177778/1‑76 (MQ=255) aGGCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTcg > 1:5443090/1‑76 (MQ=255) gCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTcgcg < 1:6411223/76‑1 (MQ=255) gCCCTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTcgcg < 2:3050572/76‑1 (MQ=255) cccTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGa > 2:5146721/1‑76 (MQ=255) cccTCTGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGa > 2:738344/1‑76 (MQ=255) tctGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATAc > 2:2508829/1‑76 (MQ=255) ctGCACAAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACg > 1:4530866/1‑76 (MQ=255) cacaAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCtt > 2:3174980/1‑76 (MQ=255) acaAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTc < 1:3940796/76‑1 (MQ=255) acaAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTc < 2:5455159/76‑1 (MQ=255) acaAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTc > 1:7433870/1‑76 (MQ=255) caAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCa < 2:6027163/76‑1 (MQ=255) caAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCa < 1:2943955/76‑1 (MQ=255) caAACACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCa > 1:1967811/1‑76 (MQ=255) aaCACCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAAc < 1:1780425/76‑1 (MQ=255) | GCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAAC > REL606/3147689‑3147834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |