| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 47.4% | 285.3 / inf | 462 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (174/69); new base T (219/0); total (393/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 505 total aligned reads displayed.
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT > REL606/1747828‑1747978 | agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGc > 2:673103/1‑76 (MQ=255) agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGc > 2:5527064/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCa > 1:7223789/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCa > 1:2289549/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCa > 2:7511823/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:524632/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:6693233/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:303460/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:4377853/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:2668424/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:1186357/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATCCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAc > 1:420173/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACCTCCAGTCAc > 1:3485642/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcc > 1:7304224/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAca > 1:6087034/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAca > 1:5447221/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:2017704/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:7538750/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:426155/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:1873361/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:1454869/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:2874572/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:148389/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:2464859/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACt > 1:5082433/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTAATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAca > 1:3695053/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcct > 1:5412541/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcct > 1:215077/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcca > 1:4042232/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcac > 1:1344555/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtt > 1:7667334/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtt > 1:7406860/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtt > 1:815972/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtt > 1:1095459/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACta > 1:5616506/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTc < 2:2187549/76‑1 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTc > 2:5030463/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTc > 2:5409092/1‑76 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCAcgat > 1:4819636/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:7474963/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:7631041/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:5693536/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:881178/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:1028870/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:5341510/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:4626563/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:4572843/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtta > 1:2369566/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTcc > 2:3365402/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACttag > 1:3200106/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACttag > 1:6069175/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACttag > 1:2959935/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACttag > 1:7088173/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACtgac > 1:5519483/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCg > 1:4980799/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCg > 2:1535334/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGTCACGccaat > 1:4500324/1‑73 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCat < 2:775542/76‑1 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATAtt > 1:3380220/1‑76 (MQ=255) tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTg > 2:4371606/1‑76 (MQ=255) atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGc > 2:1762899/1‑76 (MQ=255) atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGc > 1:3102720/1‑76 (MQ=255) ttttttttTGTTTATTTCATCCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGcc < 2:3894885/74‑1 (MQ=255) tttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCCCTCCGCCATATTTTTGCCa < 2:5784778/75‑1 (MQ=255) atTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCa > 1:6955240/1‑76 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCCCTCCGCCATATTTTTGCCATg < 2:3534898/76‑1 (MQ=255) tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATg < 1:6221455/76‑1 (MQ=255) ttttttttTCAGGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCCCTCCGCCATATTTTTGCCATGACTAt < 1:1646733/74‑1 (MQ=255) tATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:4908551/1‑76 (MQ=255) tATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 2:1170038/1‑76 (MQ=255) tCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt > 2:4679897/1‑76 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAta < 2:3233552/76‑2 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg > 1:995114/1‑76 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 2:3106330/1‑76 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 1:2522484/1‑76 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATCACTATTGATGAATgc > 2:6251618/1‑76 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 2:6999409/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 1:3821743/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAA‑CCCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg < 2:2815466/76‑1 (MQ=255) cATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt > 2:247201/1‑76 (MQ=255) ttACACGATGAACGCTTATGAC‑CTCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg < 1:6554931/76‑1 (MQ=255) ttACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg > 1:5784259/1‑76 (MQ=255) ttACACGATGAACGCTTATGAA‑CTCCAGCCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg < 1:1216177/76‑1 (MQ=255) cacGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt < 1:4144362/76‑1 (MQ=255) cGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGc > 2:6024834/1‑76 (MQ=255) cGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATCAATGCGCGACCTGGATTGc > 1:3622069/1‑76 (MQ=255) gATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGcc > 1:3261550/1‑76 (MQ=255) gAACGCTTATGAA‑CTCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg < 1:978824/76‑1 (MQ=255) ttATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt > 1:3447883/1‑76 (MQ=255) tATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg > 1:6332408/1‑76 (MQ=255) tGAC‑CTCCAGGCACCCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc < 1:3918109/76‑1 (MQ=255) gAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa > 1:1697443/1‑76 (MQ=255) aa‑CCCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg < 1:5900150/76‑1 (MQ=255) aa‑CCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg < 2:4191380/76‑1 (MQ=255) a‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt > 1:6762418/1‑76 (MQ=255) a‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt > 2:2039171/1‑76 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa > 1:1598905/1‑76 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa < 1:3546807/76‑1 (MQ=255) aGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAg > 2:1434669/1‑76 (MQ=255) gCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt < 2:7213572/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAA‑CTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT > REL606/1747828‑1747978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |