| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,044,357 | 0 | A | C | 36.1% | 359.9 / 93.5 | 196 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Reads supporting (aligned to +/- strand): ref base A (112/12); new base C (0/70); total (113/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-40 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 225 total aligned reads displayed.
CATCATGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGA > REL606/2044282‑2044431 | catcatGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCAcaccca > 1:832800/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCAcacccac < 2:2178686/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCt < 1:3971744/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCt < 1:6957265/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg < 1:4172710/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg < 1:3658992/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg < 2:5720109/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg > 2:6467491/1‑76 (MQ=255) aGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCa < 1:2754169/76‑1 (MQ=255) aGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCa > 2:309080/1‑76 (MQ=255) aGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCa > 2:7471179/1‑76 (MQ=255) gCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAg > 2:1298459/1‑76 (MQ=255) cGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGt > 1:7228348/1‑76 (MQ=255) gagaTGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTc < 2:7295987/76‑1 (MQ=255) gagaTGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTc > 1:1593781/1‑76 (MQ=255) agaTGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCa > 1:6429230/1‑76 (MQ=255) taaaaaatTTTGTTAAA‑TCG‑CCGTAGTCTAAAATAATTCGTGGACCATTCGTTTCACCCCCCCCTGCCAGTCAGGc < 2:650500/73‑1 (MQ=255) tGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGc > 1:2338432/1‑76 (MQ=255) aaaaaTTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAg > 2:4805103/1‑76 (MQ=255) aaaaTTTTTTAAA‑TTGCCCGTAGTCGAAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAg < 2:2139495/76‑1 (MQ=255) aaTTTTTTAAT‑TTG‑CCGTATTCTTAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACa < 1:5301931/76‑1 (MQ=255) aaTTTTTAAAA‑TTG‑CCGTAGTCAAAAATATTTCGTTGAGCATTCTTTTCCCCCCCCCCTGCCAGTCAGGCAAGACa < 2:2428752/76‑1 (MQ=255) tttttttAAA‑TTG‑CCGTAGTGGAAAATATTTCGTTGAGCATTCTTTTCACCCCCCCCTGCCAGTCAGGCAAGACaa < 1:629100/75‑1 (MQ=255) aTTTTTAAAA‑TCG‑CCAAAGTTTTAAATAATTGGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACaa < 1:1024221/76‑1 (MQ=255) aTTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACaa > 2:5251994/1‑76 (MQ=255) aTTTGTAAAA‑TTG‑CCGTAGTCGTAAATAATCCGTGAACCATGCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACaa < 2:7357357/76‑1 (MQ=255) ttttttAAATTTG‑CCGTAGTGTAAAATATTCCGTGGAGCATTGGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACaa < 2:1030228/76‑1 (MQ=255) tttGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAg > 2:1729641/1‑76 (MQ=255) tttGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAg > 1:6087976/1‑76 (MQ=255) ttGTTAAT‑TTG‑CCGTAGTCTAAAATAATTCGTTGAGCATTCGTTTCCCACCCCCCTGCCAGTCAGGCAAGACAAgc < 1:1691441/76‑1 (MQ=255) tGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcg > 1:3596023/1‑76 (MQ=255) tGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcg > 1:3729393/1‑76 (MQ=255) tttAAA‑TTG‑CGGTATTCTTAAAAATTTCGTTGAGCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAgcgc < 2:4879588/75‑1 (MQ=255) ttAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCa > 2:4481184/1‑76 (MQ=255) ttAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCa > 2:6290454/1‑76 (MQ=255) tAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCaa > 1:514165/1‑76 (MQ=255) aaa‑TTG‑CGGTGTTCGAAAATATTTCTTTGAGCTTTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCaaa < 2:4283027/76‑1 (MQ=255) aaa‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCaaa > 1:7621010/1‑76 (MQ=255) aa‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAg > 1:3085367/1‑76 (MQ=255) aa‑TGG‑CCGTAGTCGAAAATAATTCGTGGAGCATTCTTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAg < 2:7254451/76‑1 (MQ=255) atttG‑CCGGAGTCTTAAATAATTCTTTGAGCTTTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAg < 1:43196/74‑1 (MQ=255) tG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACCCCCACCTGCAAGTCAGGCAAGACAAGCGCAAAGTTc > 2:363665/1‑76 (MQ=255) g‑CCGTAGTCTTAAATAATTGGTTGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct < 2:2713449/76‑1 (MQ=255) g‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct > 2:3223410/1‑76 (MQ=255) gcggTAGTCGTAAATAATTCGTGGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct < 2:1329198/73‑1 (MQ=255) ccGTGGTCGTAAATAATTCGTTGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg < 1:4920534/76‑1 (MQ=255) ccGTAGTCTAAAATATTTCGTTGAGCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg < 1:2152296/76‑1 (MQ=255) ccGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg > 2:6771092/1‑76 (MQ=255) ccGTAGTCGTAAATAATTCGTGGAGCTTTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg < 2:1108481/76‑1 (MQ=255) cGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgc > 1:1098333/1‑76 (MQ=255) gTAGTCAAAAATAATTGTTTGACCATTGTTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgct < 1:7414694/76‑1 (MQ=255) tattCGTAAATATTTCGTTGAGCATTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgctg < 1:3875574/73‑1 (MQ=255) aGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 1:4681748/1‑76 (MQ=255) tCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa > 1:6748946/1‑76 (MQ=255) tCGAAAATATTTCTTTGGGCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa < 1:126639/76‑1 (MQ=255) gtttAAATATTTCGTTGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa < 2:6701791/73‑1 (MQ=255) cGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAc > 2:4455299/1‑76 (MQ=255) cGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAc > 1:4731722/1‑76 (MQ=255) tAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 1:2250373/1‑76 (MQ=255) tAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 2:526933/1‑76 (MQ=255) aaaTAATTCGTTGAGCATTCGTTTCACAGCCACCTGCCAGTCAGGCAAGCCAAGCGCAAAGTTCTGCTGAAACttt > 2:1387044/1‑76 (MQ=255) aaaTAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACttt > 2:6377018/1‑76 (MQ=255) aataatTCGTTGAGCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt < 2:2932338/76‑1 (MQ=255) atattTGGTTGAGCATTCGTTTCCCACCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTc < 2:3729639/76‑1 (MQ=255) aatttCTTTGACCATTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCt < 1:2920769/73‑1 (MQ=255) aatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTg > 1:6611515/1‑76 (MQ=255) ttCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTa > 2:6980734/1‑76 (MQ=255) tCGTTGAGCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAt < 2:3971235/76‑1 (MQ=255) gTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:1306372/76‑1 (MQ=255) ttGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa > 1:596038/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 1:2288876/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 2:4623085/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 1:6209583/1‑76 (MQ=255) gAGCTTTCGTTTCACACCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg < 2:7183955/76‑1 (MQ=255) gAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg > 1:7126542/1‑76 (MQ=255) gCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCg > 1:5716051/1‑76 (MQ=255) atgcGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag < 2:1538415/73‑1 (MQ=255) aTTCGTTTCACACCCACCTGCCAGTCAGGCCAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag > 1:6494512/1‑76 (MQ=255) ttCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgaga > 1:5062159/1‑76 (MQ=255) ttCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgaga > 2:6011843/1‑76 (MQ=255) ttCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgaga > 1:245612/1‑76 (MQ=255) cttttCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGt < 2:3164367/74‑1 (MQ=255) cGTTTCACACCCTCCTGCCAGTCAGTCAAGACAAGCGCAAAGATCTGCTGAAACTTTTCTGTATTGCGGCGAGAGt > 2:1832485/1‑76 (MQ=255) cGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGt > 2:6603407/1‑76 (MQ=255) gTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGtt > 1:5446677/1‑76 (MQ=255) gTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGtt > 1:1412551/1‑76 (MQ=255) tttCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa < 2:7621867/76‑1 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGGGTTAt > 1:587661/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 2:6184103/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 2:6817383/1‑76 (MQ=255) tCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg > 2:390591/1‑76 (MQ=255) ccaccCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg < 2:3244944/75‑1 (MQ=255) ccaccCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg < 1:996961/75‑1 (MQ=255) cacaCCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgg > 1:3394271/1‑76 (MQ=255) acacCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATggg > 2:1758416/1‑76 (MQ=255) cccccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgggg < 2:7396373/74‑1 (MQ=255) acccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGCGTTATGGGGt > 1:1250587/1‑76 (MQ=255) acccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGt > 2:5566924/1‑76 (MQ=255) cccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTc > 1:5915237/1‑76 (MQ=255) cccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTc > 1:4020001/1‑76 (MQ=255) | CATCATGAGCGAGATGCAAAAATTTGTTAAA‑TTG‑CCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGA > REL606/2044282‑2044431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |