| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,144,295 | 0 | A | C | 36.0% | 374.6 / 82.8 | 198 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (108/18); new base C (0/71); total (108/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-37 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 226 total aligned reads displayed.
CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACGG > REL606/2144220‑2144370 | cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCa < 1:5800952/76‑1 (MQ=255)cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCa > 2:2236662/1‑76 (MQ=255)cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCa > 1:2457134/1‑76 (MQ=255) gATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCAc > 2:3194502/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTGAcc > 2:7730046/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCAcc > 2:3608485/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCAca < 2:7465069/76‑2 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCt < 2:2626338/76‑1 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCt > 1:469752/1‑76 (MQ=255) tATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTg < 1:1679701/76‑1 (MQ=255) tATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAACGACTTCACCCTg < 1:5203100/76‑1 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGACTGACTTCACCCCGc > 2:2598428/1‑76 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGc > 1:7219580/1‑76 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGc > 1:5000812/1‑76 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGc > 2:3638169/1‑76 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCa > 1:2057487/1‑76 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCa < 2:4744667/76‑1 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCa > 2:561730/1‑76 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCa < 1:3882035/76‑1 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCa < 2:2824144/76‑1 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAg > 2:193221/1‑76 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAg > 2:5495235/1‑76 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGAAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAg < 2:6341201/76‑1 (MQ=255) aaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTag < 2:4460404/76‑1 (MQ=255) aaTAACCAGAATGGTTAATAGGCGTGAGACCGCGCGGAAGAAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTag < 2:3721776/76‑1 (MQ=255) aaTAAACAGAATGGTAAATAGGCGTTATCCCGCGGGGAAGGAACT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTag < 2:5704978/76‑1 (MQ=255) aTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTaga < 1:1708089/76‑1 (MQ=255) aTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTaga > 2:1495067/1‑76 (MQ=255) tAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTagag > 1:5764465/1‑76 (MQ=255) aaCCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAgagc > 2:4758824/1‑76 (MQ=255) aaCCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAgagc > 1:6034854/1‑76 (MQ=255) aCCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAgagct < 1:239461/76‑1 (MQ=255) aCCAGAATGGTGAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGCCTTCCCCCTGCCAGTAgagct < 2:4087529/76‑1 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAgagctg < 1:5878008/76‑1 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAgagctg > 1:2030135/1‑76 (MQ=255) ccAGAAGGGTTAATAGGCGTTAGCCCGGGCGGAAGGAATT‑CCCGCCTGGAATGCCTTCCCCCTGCCAGTAgagctg < 2:2860476/76‑1 (MQ=255) aGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAgagctgag > 1:596724/1‑76 (MQ=255) aTGGTTAATAGGCGTTAGCCCGCGGGGAAGAAAAT‑CTCCCCTGGAAGGACTTCCCCCTGCCAGTAGagctgagctg < 2:5132235/76‑1 (MQ=255) ggTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCa > 1:4386565/1‑76 (MQ=255) ggTAAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCa < 2:4642029/76‑1 (MQ=255) gTTAATGGGCGTTAGACCGCGCGGAAGAAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCaa < 1:757045/76‑1 (MQ=255) gTTAATAGGCGTTAGCCCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCaa < 2:5114516/76‑1 (MQ=255) gTTAATAGGCGTTAGCCCGCGCGAAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCaa < 1:3136596/76‑1 (MQ=255) ttAATGGGCGTTAGACGGCGGGGAAGGAAAT‑CCGGCCTGAAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:2159512/76‑1 (MQ=255) ttAATAGGGGTAAGACCGCGCGGAAGGAAAT‑CTCCCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:4962875/76‑1 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAc > 2:6684483/1‑76 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAc > 2:7214418/1‑76 (MQ=255) tAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcg > 2:3968371/1‑76 (MQ=255) tAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CCCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAAcg < 2:1752273/76‑1 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAAcgc < 2:4386445/76‑1 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 1:4862940/1‑76 (MQ=255) tAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCgg > 2:5678197/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:6710614/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt < 1:4149886/76‑1 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:547374/1‑76 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 2:5958250/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc > 1:7331565/1‑76 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAAT‑CTCCCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc > 2:1526250/1‑76 (MQ=255) cGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcag > 1:2875067/1‑76 (MQ=255) ttAGCCCGCGCGGAAGAAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 2:3119575/76‑1 (MQ=255) ttAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc > 1:4926476/1‑76 (MQ=255) ttAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc > 1:1150068/1‑76 (MQ=255) tAGCCCGCGCGGAAGGAAAT‑CCCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca < 2:1847163/76‑1 (MQ=255) tAGCCCGCGCGGAAGAAAAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca < 1:2761495/76‑1 (MQ=255) tAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 2:5289000/1‑76 (MQ=255) tAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 2:1446101/1‑76 (MQ=255) aGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccag > 1:188247/1‑76 (MQ=255) aGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccag > 1:1759313/1‑76 (MQ=255) gcccGCGCGGAAGAAAAT‑CCCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccagc < 1:5186440/74‑1 (MQ=255) cccGCGCGGAAGAAAAT‑CTCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCt < 1:789113/75‑1 (MQ=255) ccGCGCGGAAGAAAAT‑CTCGCCTGGAAGGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTg < 1:1387936/76‑1 (MQ=255) gcgcgAAAGAAAAT‑CTGCCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGc < 1:7006232/76‑1 (MQ=255) cggggaaggaaTT‑CGCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 1:7465241/73‑1 (MQ=255) ggaagaaaTT‑CTCGCCGGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa < 2:5990627/76‑1 (MQ=255) ggaagaaaAC‑CTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa < 2:2983228/76‑1 (MQ=255) gaaggaaCT‑CCCGCCGGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 2:2388826/76‑1 (MQ=255) gaaggaaAT‑ATCGCCTGGAATGTCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 1:5666101/76‑1 (MQ=255) aaggaaAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa > 1:6366406/1‑76 (MQ=255) aggaaAT‑CTCGCCTGGATTGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 1:1183952/76‑1 (MQ=255) aggaaAT‑CTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 1:3564492/76‑1 (MQ=255) aggaaAT‑CTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 2:7604484/76‑1 (MQ=255) aagaaATCCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa < 2:6930063/74‑1 (MQ=255) gaaaaT‑CTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATa < 1:1020213/74‑1 (MQ=255) gaaATCCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATa < 2:2721740/76‑1 (MQ=255) gaaAT‑CTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:3399863/76‑1 (MQ=255) aaaaT‑CTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 1:6124960/75‑1 (MQ=255) aact‑ctcCCCGGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGc < 2:6528444/73‑1 (MQ=255) aaaT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGc > 2:2110807/1‑76 (MQ=255) aaaT‑CCCGCCGGAAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGc < 1:6582825/76‑1 (MQ=255) aT‑CCCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCgg < 2:7173841/76‑1 (MQ=255) ctcGCCGGAAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTa < 2:3249338/76‑1 (MQ=255) cccGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTa < 1:7495600/74‑1 (MQ=255) cGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaaa > 1:7304141/1‑76 (MQ=255) gCCTGAAATACCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAAt < 1:644575/76‑1 (MQ=255) gAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGct > 1:290248/1‑76 (MQ=255) gAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGct < 2:3674937/76‑1 (MQ=255) aaTGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGctc < 2:1673343/76‑1 (MQ=255) gACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTcc > 2:479521/1‑76 (MQ=255) cTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCgg > 1:6249259/1‑76 (MQ=255) aCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACgg > 1:901537/1‑76 (MQ=255) | CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAAT‑CTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACGG > REL606/2144220‑2144370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |