| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 36.1% | 192.3 / 38.4 | 122 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (13/65); new base G (42/2); total (55/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.87e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 170 total aligned reads displayed.
CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 | ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtggt < 1:6645669/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑gggggg > 1:5604840/1‑75 (MQ=255) cAGATGAGGCGAACTGTAGCCGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑Ggtggtg < 2:3346280/76‑1 (MQ=255) tgATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGCGGGGc > 1:6062493/2‑76 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑Gggggtgg > 2:2058606/1‑75 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGAGGGGc > 1:1926683/1‑76 (MQ=255) gATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGcc > 1:5711781/1‑76 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCt < 2:3042897/76‑1 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGccc > 1:2062995/1‑75 (MQ=255) tGAGGCGACCTTTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCtt < 2:342661/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCtt < 1:2934141/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCtt < 1:804182/76‑1 (MQ=255) gAGGCGAACTGTAGTCGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 1:5198500/76‑1 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGc > 1:6705665/1‑76 (MQ=255) gcaaaCTGGAGACGCCCATTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGcc < 1:6892695/73‑1 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGGCa > 2:5489795/1‑76 (MQ=255) gAACTGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCAt < 2:43805/76‑1 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGTCAt > 1:3206209/1‑76 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCAt > 1:7525446/1‑76 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCAt > 1:896412/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCAtt < 2:4138378/76‑1 (MQ=255) cTGTAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCg < 1:3050912/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCCTTGCCATTCg > 2:3077844/1‑76 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCg > 1:3337333/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGGCCTTTGCCATTCGt > 2:470608/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTa > 2:1766381/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTa > 1:3723853/1‑76 (MQ=255) tAGACGCCCCCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAc < 2:7436518/76‑1 (MQ=255) tAGACGCCCATTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAc < 1:851471/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGGCTTTGCCATTCGTAc > 1:4048116/1‑76 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTCTTTCATTCGTAc > 1:1030937/1‑76 (MQ=255) cccTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCGTTCGTACCCGCAACg < 1:762418/74‑1 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAAcc > 1:5732118/1‑75 (MQ=255) cttttGTCCCCTGCCCCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACgg < 2:3037263/74‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGGACCCGCAACGGTa > 1:7141991/1‑76 (MQ=255) tGTCCCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa < 1:1569733/76‑1 (MQ=255) gtccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAac < 1:3640450/73‑1 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCATTCGTACCCGCAACGGTaaaa > 1:7133924/1‑75 (MQ=255) tccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 2:5066375/74‑1 (MQ=255) tccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 1:5473062/74‑1 (MQ=255) tccccTGCCGCCATCAAAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 1:4117905/74‑1 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTTGCATTTCTACCCCCAACCGGAAaca > 1:2780664/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCCACGGGAAaca > 1:5885476/1‑76 (MQ=255) cccccTGCCGCCATCAAAATCGACCCCAGCGTACGGAA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 1:1255022/74‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTTTCC‑TTCGTAACCACCAAGGGAAacac > 2:873769/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCCTATCCGCAATGGTAAaaac > 1:361508/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGTCATTCGGACCCGCCACGGTAAacac > 1:4589312/1‑76 (MQ=255) cccTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAcaca < 2:4608045/76‑1 (MQ=255) cccTCCCGCCATCAAAATCGCCTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAcaca < 2:267125/76‑1 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:1035746/76‑1 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:3478221/76‑1 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:3795887/76‑1 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCCACCGTAAACACaa > 2:7567641/1‑76 (MQ=255) ccTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:5479132/76‑1 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCGTTCCCACTCGCAACCGCAACGGGAAAACCAAAt > 1:4818560/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCCTACCCGCAACGGGAAACACAAAt > 1:6180338/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGCCATTCGTACCCGCAAAGGTAAACACaaaa > 1:4456443/1‑75 (MQ=255) ggcccccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:2243398/75‑1 (MQ=255) cgccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:3454245/75‑1 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCCACGGTAAACAAAAATg > 1:211776/1‑76 (MQ=255) cccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg < 2:35764/75‑1 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCCACGGGAAACACAAATGGCGCt > 1:6352530/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGTGGTCCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg < 2:6889970/76‑1 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCCTTCGTACCCGCAACCGTAAACACAAAAGGCGCTg > 2:4239781/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg > 1:2430853/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGt < 2:930772/76‑1 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGt < 1:4967768/76‑1 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTAGCCGCAACGGTAAACACCAATGGCGCCggg > 2:2748438/1‑75 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCCCAACGGGAAACACAAAAGGCGCTGGt > 1:7339508/1‑76 (MQ=255) aGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTgggt > 2:2483173/1‑74 (MQ=255) gAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 1:732431/76‑1 (MQ=255) gAAGCGCCTCAAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:1493506/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCAGTCGTACCCGCAACGGTAAACACAAAAGGCGCTGGTTTc > 1:3972354/1‑76 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 2:1269288/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 2:5265600/76‑1 (MQ=255) tCGTCTCCAGCGTTCTGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 2:7373109/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:6761233/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:6604970/76‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:6545427/76‑1 (MQ=255) gccTCCAGCTTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 2:6798158/74‑1 (MQ=255) gACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCCTTCGTACCCGCAACGGTAAACACAAAAGGCGCGGGGTTCAGGa > 1:5927459/1‑76 (MQ=255) cTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg < 1:1311666/76‑1 (MQ=255) cTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg < 1:1100719/76‑1 (MQ=255) cTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAGGACg > 1:1727062/1‑76 (MQ=255) cTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGagg > 1:1363519/1‑74 (MQ=255) cAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 1:972943/76‑1 (MQ=255) cAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 2:2647811/76‑1 (MQ=255) cAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa > 2:3772791/1‑76 (MQ=255) gggTACGGCA‑GGTGGTGCCTTTGCCATTCGTTCCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:1071319/74‑1 (MQ=255) gCGTACGGCA‑GGTGGTGCCTTTGCCATTCATACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:261351/76‑1 (MQ=255) cGTACGGCA‑GGTGGGGGCTTTGGCATTCGGACCCGCAACGGTAAACACAAATGGCGCTGGTTTTATGAAGCCAAGa > 2:120972/1‑76 (MQ=255) cGTACGGCA‑GGGGGGGGCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTTAGGACGCCAAGa > 2:6546585/1‑76 (MQ=255) gTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc > 2:5410020/1‑76 (MQ=255) cA‑GGTGGTCCCTTTCCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg < 1:6558604/76‑1 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 2:2039166/1‑76 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 2:6360526/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 2:6882954/76‑1 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:1249803/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:6218072/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:2773215/1‑76 (MQ=255) | CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |