| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,515,909 | 0 | A | C | 48.6% | 69.1 / 47.0 | 72 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (25/12); new base C (0/35); total (25/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 103 total aligned reads displayed.
TAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515836‑2515984 | tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGG‑TTTTTCGaccccc > 2:3965363/1‑76 (MQ=255)tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGaccacc < 1:807886/76‑1 (MQ=255)tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGaccacc < 2:3102244/76‑1 (MQ=255) aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGAccacca < 2:5754598/76‑1 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag < 2:4611552/76‑1 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag < 1:3564798/76‑1 (MQ=255) caacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag‑c < 2:260395/76‑1 (MQ=255) caacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag‑c < 2:3650496/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag‑cc < 1:3858915/76‑1 (MQ=255) acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCAccag‑cca < 1:2177357/76‑1 (MQ=255) tggagaAAAGCGCGAAACCAGTTGATGCAAAAAGGGCGCAGGTATGGCGGCGGT‑TTCATCGCCCCCCAG‑CCAGTCg < 2:1386601/74‑1 (MQ=255) tggAGGAAAGCGCGATACCAGTTGATGCAACAGGGGCGCAGGTATGGCGGCGGT‑TTCCTCGCCCCCCAG‑CCAGTCg < 2:1373616/74‑1 (MQ=255) tCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGG‑TTCATCGACCACCAC‑CCAATCg > 1:6601167/1‑76 (MQ=255) cGAGTAAAGCGCGATCCGTGTTGAGGCAACAAGGGCCCGGGTTTGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCgg < 2:3525178/76‑1 (MQ=255) aGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTTAACGACCCCCCG‑CCAGTCGGCt > 1:7718783/1‑76 (MQ=255) ggAAAGCGAGATACGAGTTGATCAAACAAGGGCGCAGGTATGGCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCtt < 2:6608224/76‑1 (MQ=255) gagaaaCCCTATACCAGTTGATGAACAAATGCCGCAGGTTGGCCGGCGAT‑TTCATCGACCCCCAG‑CCAGTCGGCtt < 2:7046334/73‑1 (MQ=255) gaaaagCGCGATACCGGTTGATGCAACAGGGGCGCAGGTATGGCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCtt < 2:5042985/74‑1 (MQ=255) gAAACCGCAATCCAAGTTGATGCAACAATGGCGCAGGTATGTCGGCGGC‑TTCATCACCCCCCAG‑CCAGTCGGCTTg < 2:1171794/76‑1 (MQ=255) aaaaaCGCGTTCCCATTGGATGAAACAAGGGCGCGGGTAGGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTg < 2:3028491/75‑1 (MQ=255) aaaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGG‑TTTATCGGCCACCAG‑CCAGGCCGCTTGc > 1:1673069/1‑76 (MQ=255) aaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGCTTTCACCG‑CCACCAGCCCAGTTGGCtgct > 1:4450748/1‑73 (MQ=255) gcgATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATTCACCCCCAG‑CCAGGCGGCTTTCTCATc > 1:5509742/1‑76 (MQ=255) gcgATACCAGTTGATGAAACAATGGCGCAGTTAGGCCGGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATc < 2:3207435/76‑1 (MQ=255) cgATCCCAGTTGATGCAACAATGGCGCAGGTTTGGCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCg < 1:5742792/76‑1 (MQ=255) cgATCCCAGTTGATCCAACAAGGGCGCGGGTATGGCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCg < 1:1035222/76‑1 (MQ=255) aTACCAGTTGATGCAACAATGGCGCAGGTAGGGCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCt < 1:825533/76‑1 (MQ=255) cggTTGATGCAACAGGGGCGCAGGTAGGGCTGCGGC‑TCCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCt < 2:338169/74‑1 (MQ=255) tgtGATACAAAAATGGCGCGGGTATGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑aa < 2:850448/74‑1 (MQ=255) gTTGATGCAACAATGGCGCAGGTATGGCTGCGGT‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑aa < 2:7682545/76‑1 (MQ=255) ttGATGCAAAAATGGCGCAGGTATGGCTGCGGC‑TTCATCGCCCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AAc < 2:1913718/76‑1 (MQ=255) ttgtGCAAAAAGGGCGCAGGTAGGGCTGCGGC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACa < 2:1106768/73‑1 (MQ=255) tGATGCAACAATGGCGCAGGTATGGCTGCGTC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACa < 1:962090/76‑1 (MQ=255) gaggCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 1:1502735/73‑1 (MQ=255) gATGCAACAAGGGCGCAGGTTTGCCTGCGGC‑TTCATCGCCCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 1:5442799/76‑1 (MQ=255) gATGAAACAGTGGCGCAGGTGGGGCGGCGCC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 2:2113760/76‑1 (MQ=255) tcacaaaaaGGGCTCAGGTTTGGTTGCGGT‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAg < 2:54428/73‑1 (MQ=255) aTGCAACAATGGGGCAGGTAGGGCTGCGGC‑TTCACCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAg < 1:7706257/76‑1 (MQ=255) tGCAACAGGGGGGTAGGTATGGCTCCGCC‑TTCACCCACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAgg < 2:7522601/76‑1 (MQ=255) gCAACAAGGGCGCGGGTTTGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGc < 2:4899903/76‑1 (MQ=255) aacaaGGGCGCAGGTATGGCTGCGTC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCAt < 2:3777263/76‑1 (MQ=255) acaaTGGCGCAGGTATGGCTGCGGC‑TTCATCGACCACCAG‑GCCGTCGGGTTTCTCACCCCGGGCT‑AACAAAGCaac > 1:7710472/1‑74 (MQ=255) caaTGGCGCGGTTGTGGCTGCGGC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATcc < 1:4865464/76‑1 (MQ=255) aagggCGCGGGAGTGGCTGGGCC‑TTCACCCCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCg < 2:1241866/73‑1 (MQ=255) agggCGCAGGTAGGGCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCgg < 1:4782278/74‑1 (MQ=255) aTGGCGCAGGTATGGCTGGGGC‑TTTTTCCACCACCCG‑CCAGGCGGCCTTCTCCTCGCCGGCTAAACAAGGCACCCg > 2:5649637/1‑76 (MQ=255) aTGGCGAAGGTAGGGCTGCGGC‑TTCATCGCCCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCgg < 1:1932709/76‑1 (MQ=255) gggCGCAGTTGGGGCTGCGCC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGgc < 1:5172404/75‑1 (MQ=255) gcAGGTTTGGCGGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCaa < 1:3837794/76‑1 (MQ=255) gaGGTATGCGTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAAt < 2:332964/75‑1 (MQ=255) cgggTACGCCTGCGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAAt < 2:7461508/74‑1 (MQ=255) gggTATGGTGGCGGC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATa < 1:5129248/75‑1 (MQ=255) gggTATGGCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATa < 1:1180291/75‑1 (MQ=255) ggTATGGCTGCGCT‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATaa < 1:5195287/76‑1 (MQ=255) ggTATGCCTCGGGC‑TTCATCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATaa < 1:7671233/76‑1 (MQ=255) tagggCTGCGCC‑TTACGCAACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAg < 1:768258/73‑1 (MQ=255) tATGGCTGCGGC‑TTTATCGACCCCCCG‑CCCGGCGGGTTGCTCATTGCTGGGT‑AAAAAAGGACCCGGCGCGACaaaa > 2:1545373/1‑75 (MQ=255) tATGCCGGCCGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAg < 2:7038423/76‑1 (MQ=255) tgggCTGCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGc < 1:7579401/74‑1 (MQ=255) ctGGCTGCGCC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGc < 1:2583459/75‑1 (MQ=255) tGGCGGCGGC‑TCCACCCCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCg < 1:4319241/76‑1 (MQ=255) tGGCCGCGTC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCg < 1:6057933/76‑1 (MQ=255) gggCTGCGCC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCg < 2:4031976/75‑1 (MQ=255) ggCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTTGTCCTCGCTGGCC‑AACAAGGGAACCCGGGCCATAAAGCGa > 2:757236/1‑76 (MQ=255) ggCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTTCTCATCGCTGGCT‑AACAAGGCAACCGGCGGCAAAAAGCGa > 1:2463357/1‑76 (MQ=255) ggCTGCGGC‑TTCATCCCCCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGa < 2:5587745/76‑1 (MQ=255) gCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGGT‑AACAAGGCATCCGGCGCAAAAAAGCgga > 1:1342511/1‑74 (MQ=255) gCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCCATAAAGCgca > 1:7244132/1‑74 (MQ=255) gCTGCGGC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGaa < 1:6402324/76‑1 (MQ=255) cTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGGTCATCGGCGGCT‑AACAACGCATCCCGCGGAATAAAACGgaa > 2:5348555/1‑76 (MQ=255) cTGCGCC‑TTCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGaaa < 2:4119668/76‑1 (MQ=255) tGCGGC‑TTTATCGACCACCAG‑CCAGTCGGCTTGGTCCTCGCTGGGT‑AAAAAGGCATCCGGGGCCAAAAAACaaaaa > 2:5546010/1‑75 (MQ=255) gCGGC‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACt < 2:1301268/76‑1 (MQ=255) cGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAACGAAACtt > 1:6327189/1‑76 (MQ=255) ggC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGGAACTtc > 1:398641/1‑76 (MQ=255) ggC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtc > 2:435594/1‑76 (MQ=255) gC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtct > 2:2533016/1‑76 (MQ=255) gtttCACCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtct < 2:4084162/74‑1 (MQ=255) c‑TTCATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc < 2:1550238/76‑1 (MQ=255) c‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 2:3092911/1‑76 (MQ=255) c‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCCGGCT‑AACAAGGCATCCGGCGCAATAAAGACAAACTtttc > 1:7073683/1‑76 (MQ=255) ttCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 2:2225723/1‑76 (MQ=255) ttCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:876157/1‑76 (MQ=255) tCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc > 1:534404/1‑76 (MQ=255) tCACCGACCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc < 2:4006750/76‑1 (MQ=255) cATCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc < 2:6480776/76‑1 (MQ=255) cATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc > 1:6078454/1‑76 (MQ=255) cATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc > 1:3450374/1‑76 (MQ=255) cATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc > 1:2341853/1‑76 (MQ=255) cATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc > 1:6087666/1‑76 (MQ=255) aTCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc > 2:5368944/1‑76 (MQ=255) aTCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc > 2:7470128/1‑76 (MQ=255) aTCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc > 2:427890/1‑76 (MQ=255) aTCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACATCTCcccc > 2:4130293/1‑76 (MQ=255) tCGCCCCCCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg < 2:5938369/76‑1 (MQ=255) tCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg > 2:737766/1‑76 (MQ=255) cGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTTGCCCGc > 2:966969/1‑76 (MQ=255) cGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc > 2:636566/1‑76 (MQ=255) caccaG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAt > 1:7507397/1‑76 (MQ=255) accaG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt > 2:6613915/1‑76 (MQ=255) | TAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGC‑TTCATCGACCACCAG‑CCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515836‑2515984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |