| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,765,150 | 0 | A | C | 34.3% | 272.2 / 34.4 | 134 | K528N (AAA→AAC) | recG | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (74/14); new base C (0/46); total (74/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.88e-24 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 174 total aligned reads displayed.
GGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTAT > REL606/3765075‑3765224 | gcggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAAc > 1:1268653/1‑76 (MQ=255) cggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTCGTACACGGGCGGATGAAAcc > 2:7008821/1‑76 (MQ=255) ggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCt < 1:7039909/76‑1 (MQ=255) ggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCt < 1:2289661/76‑1 (MQ=255) aaGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGc > 1:510076/1‑76 (MQ=255) aaGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACATTGGCCTGGTACACGGGCGGATGAAACCTGc > 2:6191336/1‑76 (MQ=255) cTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCga > 1:6793635/1‑76 (MQ=255) aCCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCgaga > 1:6974333/1‑76 (MQ=255) aCCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCgaga > 1:1872882/1‑76 (MQ=255) aCCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCgaga > 1:3046558/1‑76 (MQ=255) tgtGGGAAGAGTTGAAGCTGGCGCTACCAGATTTGACCGTTGGCCGGGTACACGGGCGGGGAAAACCTGCCGAGaa < 2:1924401/74‑1 (MQ=255) ccTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGaa > 1:7728638/1‑76 (MQ=255) cTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGaaa > 2:1402440/1‑76 (MQ=255) cTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGaaa > 2:1842594/1‑76 (MQ=255) gggAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACa > 2:7539292/1‑76 (MQ=255) gggAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACa > 1:7246074/1‑76 (MQ=255) gggAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACa > 2:4412529/1‑76 (MQ=255) gaaaGAGTTAAACCTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACAGGGGGGGATGACCCCTGCCGAGAAACAg < 2:168804/74‑1 (MQ=255) gAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAgg > 1:2583396/1‑76 (MQ=255) aaGAGTAGAAACGGGCGCTGCCAGAGTTGAACGTTGGCCGGGTACCGGGGCGAATGAACCCTGCCGAGAAACAGGc < 2:4944101/76‑1 (MQ=255) agagTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCg > 2:2361903/1‑76 (MQ=255) ggTTGAAACGGGCGCTACCAGAGTTGAACGTGGCCCTGGTACGCGGGCGGATAAAACCTGCCGAGAAACAGGCGGt < 2:3229043/75‑1 (MQ=255) agTTGAAGCGGGCGCTACCAGAGTTGAACGTTGCCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGt < 2:948826/76‑1 (MQ=255) agTGGAAACGGGCGCTCCCAGAGTTGATGTTTGGCCTGGTAACGGGGCGGATAAACCCTGCCGAGAAACAGGCGGt < 2:2969214/76‑1 (MQ=255) tGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGAt > 1:6551404/1‑76 (MQ=255) tGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGAt < 2:3562671/76‑1 (MQ=255) gAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATg > 1:4436181/1‑76 (MQ=255) gAAACGGGCGCTACCAGAGTTAAACGTGGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATg < 1:7334658/76‑1 (MQ=255) aaaCTGGCGCTACCAGAGTTGAACATTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATgg > 2:5374892/1‑76 (MQ=255) aaaCGGGCGCTACCAGAGTGGATCGTGGGCCTGGTCCACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATgg < 2:657076/76‑1 (MQ=255) aaaCGGGCGCTACCAGAGTGGACCGTTGCCGTGGTCCAGGGGCGGAGGAAACCTGCCGAGAAACAGGCGGTGATgg < 1:132349/76‑1 (MQ=255) accTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACAGGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGc < 2:3745350/74‑1 (MQ=255) accTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGc < 1:796860/74‑1 (MQ=255) aaCTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATggt > 2:1408272/1‑75 (MQ=255) aCTGGCGCTACCAGATTTGACCGTTGGCCTGGAACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGcg < 1:1550043/76‑1 (MQ=255) cTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATGGcgt > 2:6838131/1‑76 (MQ=255) tGGCCCTCCCGGAGTTGAACGTTGGCCGGGTACAGGGGGGGATGAACCCTGCCGAGAAACAGGCGGTGATGAcgtc < 1:4832028/76‑1 (MQ=255) gggCGCTACCAGAGTTGAACGTTGGCTTGGTACCCGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGcgtc < 2:3052057/75‑1 (MQ=255) gcgcTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATCGcgttct > 1:5824993/1‑73 (MQ=255) gcTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGttt > 1:45088/1‑76 (MQ=255) gcTACAGGAGTTGAACGTTGGCCTGTTACAGGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGttt < 2:4098376/76‑1 (MQ=255) ccTACCAGAGTGGACGTTTGGCTTGGTACACGGGCGGAGAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGttt < 2:6974333/75‑1 (MQ=255) cTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTa > 1:6135164/1‑76 (MQ=255) cTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGGCGTTTa > 1:3321353/1‑76 (MQ=255) cTACAAGATGTGAATGTTGCCCGGGGTCACGGGGGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTa < 2:6142426/76‑1 (MQ=255) tACCAGAGTTGACCGTGGGCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTaa < 1:2702820/76‑1 (MQ=255) aCCAGATTTGAGCGTTGGCGTGGTACACGGGGGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTaaa < 1:4293000/76‑1 (MQ=255) ccAGATTTGAACGTGGGCCGGGTACACGGGGGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAAc < 2:2471528/76‑1 (MQ=255) ccAGAGTTGAACGTTGCCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAAc < 2:4856602/76‑1 (MQ=255) cAGATTTGACCGTTGGCCGGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACa < 1:6464703/76‑1 (MQ=255) cAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATGGCGTCGTTTAAACa > 1:4708312/1‑76 (MQ=255) cAGAGTGGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACa < 1:1902365/76‑1 (MQ=255) cAGAGTGGAACGTGGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACa < 1:6263212/76‑1 (MQ=255) agagTTGAACGTTGGCGTTGTCCCGGGGGGTAGCAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACaa < 1:5265027/76‑1 (MQ=255) agTTGAAGTTTGGCCTGGTACACGGGCGAATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAgg < 1:114074/76‑1 (MQ=255) tttGACCGTTGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGt < 1:7349455/75‑1 (MQ=255) ttGAACTTGGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTg < 2:6581605/76‑1 (MQ=255) ttGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGGAGGCGTGGTTTAGACAAggcg > 2:4885728/1‑74 (MQ=255) ttGAACGTTGGCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTg < 2:1576261/76‑1 (MQ=255) tGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGGTGGCGTCGTTTAAAAAAGGGGa > 1:6963698/1‑76 (MQ=255) tGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGAAGGCGTCGATTAAACAAGGTGa > 2:3092146/1‑76 (MQ=255) gAACGTTGGCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAg < 2:2330201/76‑1 (MQ=255) aCGTTGGCCTGGTCCACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCt < 1:356708/76‑1 (MQ=255) aCGTTGGCCTGGTACACGGGGGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCt < 1:3778124/76‑1 (MQ=255) aCGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGGGTCGTTTAAACAAGGGGAGCt > 2:4285157/1‑76 (MQ=255) aCGTTGGCCTGGTACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCt < 2:6382119/76‑1 (MQ=255) cGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGGTGGCGGCGTTTAAACAAGGGGAGcca > 2:6686762/1‑74 (MQ=255) cGTGGGCCTGGTACGCGGGCGGAGAAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTa < 2:4491855/76‑1 (MQ=255) ttGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATGGCGGCGGTTTAACAAGGGGAGCTaca > 1:1012216/1‑76 (MQ=255) tGGCCTGGTACACGGGGGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTacac < 1:4830971/76‑1 (MQ=255) tGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATGGCGTCGTTTAAACAAGGTGAGCCacac > 2:7649667/1‑76 (MQ=255) tGGCCCGTTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTacac < 1:7530058/76‑1 (MQ=255) ggCCTGGTACGCGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACAcc < 1:5867135/76‑1 (MQ=255) gCCTGGTACCGGGGGGGACAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACct < 1:4885207/76‑1 (MQ=255) gCCGGGTACACGGGGGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACct < 2:570656/76‑1 (MQ=255) ccTGGTACAGGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACctg < 2:2196577/76‑1 (MQ=255) tGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACctgct > 1:1668381/1‑76 (MQ=255) tGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACctgct > 1:2488751/1‑76 (MQ=255) acgcGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGtt < 1:5750325/73‑1 (MQ=255) acacGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGtt > 1:3679757/1‑76 (MQ=255) gaggggCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTg < 2:1775500/73‑1 (MQ=255) cgggggCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTg < 2:2776196/73‑1 (MQ=255) cGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATTGCGTCGTTTAAACAAGGGGAGCTACACCTGCTGGGTGcc > 1:6417924/1‑76 (MQ=255) cGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGATGGCGTCGTTTAAAACAGGGGAGCTACACCTGCTGGTTGca > 2:4449599/1‑75 (MQ=255) gggCGGATGAAACCTGCCGAGAAACAGGCGGGGGTGGCGCCGTTTAAATAAAGTGAGCTCCACCTGCTGGTTggcc > 2:7220698/1‑73 (MQ=255) ggCGAAAGAATCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCcac < 1:7316274/76‑1 (MQ=255) gCGGATAACCCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCcaca < 2:2914391/76‑1 (MQ=255) gCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCcaca < 2:958863/76‑1 (MQ=255) gCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCcaca < 1:4557593/76‑1 (MQ=255) gggATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACaa < 2:4613465/75‑1 (MQ=255) gggATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACaa < 1:5208289/75‑1 (MQ=255) gATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAAcc > 2:1021857/1‑76 (MQ=255) gATGAAACCTGCCGAGAAACAGGCGGGGATGGCGTCGTTTAAACAAGGTGAGCTAAACCTGCTGGGTGCCACAAcc > 2:2835221/1‑76 (MQ=255) aTGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGGGCTACACCTGCTGGTTGCCACAACCg > 1:3064526/1‑76 (MQ=255) aTGAAACCTGCCGAGAAACAGGCGGGGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCg > 2:7666426/1‑76 (MQ=255) tGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGGTGCCACAACCGt > 1:1060987/1‑76 (MQ=255) agAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGt < 1:1400189/75‑1 (MQ=255) aaaCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTa > 1:5398044/1‑76 (MQ=255) aaCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTAt > 2:6485550/1‑76 (MQ=255) aaCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTAt > 1:2409953/1‑76 (MQ=255) | GGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTAT > REL606/3765075‑3765224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |