| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 34.2% | 190.9 / 88.8 | 146 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (66/30); new base T (0/50); total (66/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 217 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | aGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTc > 2:7507075/1‑76 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCt > 1:2404172/1‑76 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 1:6960328/76‑1 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 1:4504801/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGCTTTTCTa > 1:5082737/1‑76 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 1:1476013/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 2:4752115/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGCAGGTGCTGGCTGGGATACCGATTTTCTAgac > 2:3226473/1‑74 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGAAGCGATTTTTTAgac > 2:5959937/1‑74 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:1291762/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 2:5681996/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:5286691/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 1:344055/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:538637/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg > 1:5669822/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGt > 2:7051878/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGAAGCGATTTTCTAGcccgc > 1:4842269/1‑73 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTTGCCAGTa > 1:3046818/1‑76 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTa > 2:5309421/1‑76 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTa > 2:1169184/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTTTAGCCAGGAt > 1:2025460/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGGAGCGGTTTTTTTGCCAGGAt > 1:7332141/1‑76 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTAtt > 2:2317010/1‑76 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAtt > 1:5045913/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTc > 2:355434/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTTGTc > 2:2738862/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTc > 2:4563487/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTca > 1:5032401/1‑76 (MQ=255) cTGAGGGCGCGCTCGGCAAGGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTca < 1:961081/76‑1 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGGCAGTATTGGTcag > 2:5640578/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTccgg > 2:2660967/1‑73 (MQ=255) tgccgcgcTCGGCGAGGCGGTAACGCGTGAAGGTGCTGGCTGGAATAGCATTTTTTTAGCCAGTATTGGTcagcag < 1:3385688/73‑1 (MQ=255) tGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTcagcag < 1:5567973/76‑1 (MQ=255) gcgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGtt < 1:3597174/76‑1 (MQ=255) gcgcgcTCGGCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGtt < 1:4010388/76‑1 (MQ=255) cgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa > 2:6694402/1‑76 (MQ=255) cgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa > 1:5233920/1‑76 (MQ=255) cgcgcTCTGCGAAGCGGAAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTa < 1:777897/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGGTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGa < 2:5901722/76‑1 (MQ=255) gcTCGGCGAGGCGGTAACGCGTGAAGGGGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGa < 2:1768838/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:4954027/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:1913917/76‑1 (MQ=255) ctcgGCGAAGCGGTACCGGGTGAAGGGGCGGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:2455649/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGTTGCTGGCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 1:3468407/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCCGCAGTTAGGAACg > 1:3499999/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTGGTCAGCAGTTAGGaaag > 2:7228925/1‑74 (MQ=255) tGCGAAGCGGTAACGCGAGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTGAGCACGTAGGAACgg > 1:5789376/1‑76 (MQ=255) gCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc > 2:7278856/1‑76 (MQ=255) ggAAGCGGTAACGCGTGAAGGTGTGGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt < 1:6386602/75‑1 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt > 1:6798331/1‑76 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt > 2:3522561/1‑76 (MQ=255) gAAGCGGTAACGGGGGAAGGTGCGGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg < 1:5364810/76‑1 (MQ=255) gAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg < 1:2463544/76‑1 (MQ=255) gAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGTTTTATAGCCAGGATTGGGCAGGCGGTAGGAACGGCTg > 1:1370644/1‑76 (MQ=255) gAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg > 1:1795134/1‑76 (MQ=255) aaGCGGTAACGGGTGAAGGTGTTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 1:2669814/76‑1 (MQ=255) aaGCGGTAACGCGTGAAGGTGCTGGCTGGGAATGCGATTTTCTAGCCAGTAATGGTCAGCAGATAAGAACCGCTgt > 2:3444369/1‑75 (MQ=255) gTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTGGCCAGTATTGGTTAGCAGTTAGGGAAGGCTGAATccc > 1:7393990/1‑76 (MQ=255) tAACGGGGGAAGTTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt < 2:4989235/76‑1 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt > 1:1298224/1‑76 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCtt > 1:4906082/1‑76 (MQ=255) cgcgGGAAGGTGTTGGCGGGGATGGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 2:7320004/76‑1 (MQ=255) gcgTGAAGGTGCTGGCTGGAATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:5189057/76‑1 (MQ=255) cgTGAGGGTGCTGGCGGGGATGGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAc < 1:3944767/76‑1 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt < 2:4137411/76‑1 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTTAACt > 2:4716466/1‑76 (MQ=255) gagggTGCGGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg < 2:2366078/73‑1 (MQ=255) gAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCCTTAACTgg > 2:2234946/1‑76 (MQ=255) atggTGCTGGTTGGGAGAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg < 1:3204165/74‑1 (MQ=255) aaGGTGGTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg < 2:2558409/76‑1 (MQ=255) aaGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg > 2:1841713/1‑76 (MQ=255) gttGCGGGGTGGTATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggc < 1:2450268/74‑1 (MQ=255) ggTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggc < 2:1674707/76‑1 (MQ=255) ggTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggc > 1:4362535/1‑76 (MQ=255) gTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGGATCCCTTTAACTGGggcg > 1:2114780/1‑76 (MQ=255) gTGCGGGGTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 2:2076435/76‑1 (MQ=255) gctggctgGGGTAGGGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgg‑c > 2:642569/1‑76 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑Ct > 2:1255996/1‑76 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑Ct > 1:6312239/1‑76 (MQ=255) gggcgggGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTg < 2:114706/75‑1 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTgg > 1:1007644/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTgg > 2:2989844/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTgg > 1:6478213/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTgg > 1:3296408/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCCTGAACTGGGGCGG‑Cttg > 1:2927493/1‑74 (MQ=255) ttggGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCg < 2:6902496/75‑1 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGa > 2:4801179/1‑76 (MQ=255) gggATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGaa < 1:2776410/76‑1 (MQ=255) ggATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAAt < 2:5032401/76‑1 (MQ=255) gaaTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAAt < 1:254342/74‑1 (MQ=255) aTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAAtgt < 2:3324632/76‑1 (MQ=255) aTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAAtgt < 2:7124280/76‑1 (MQ=255) tggCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAAtgtg < 1:6168994/74‑1 (MQ=255) cgtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAAt < 1:5168086/73‑1 (MQ=255) cgtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAAt < 2:6278493/73‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAATcc < 2:4011555/75‑1 (MQ=255) tttttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAATcc < 1:2846351/75‑1 (MQ=255) aTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCCGGGCGAAGGTGATTc > 2:6207158/1‑76 (MQ=255) ttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGGATTCGcc > 1:4272413/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATTGGAATCcccccc > 2:3016720/1‑74 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG‑CTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |