| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,924 | 0 | A | C | 99.1% | 916.9 / ‑4.7 | 232 | V461G (GTC→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (147/82); total (148/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 240 total aligned reads displayed.
CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCG > REL606/473851‑473995 | ccATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcg > 2:278835/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 2:4477765/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 2:4163011/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 1:3059996/1‑76 (MQ=255) tttGCACATTCCAGATGCGCCAGTGGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGct < 2:5307901/76‑1 (MQ=255) ttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctc > 1:6931580/1‑76 (MQ=255) ttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctc < 2:1365573/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:1435195/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:5904087/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:1276080/76‑1 (MQ=255) gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc < 2:2684837/76‑1 (MQ=255) gCACATTCCAGATGCCCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc < 2:3823719/76‑1 (MQ=255) cacaTCCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg < 2:289345/76‑1 (MQ=255) acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:7371972/76‑1 (MQ=255) acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:7104635/76‑1 (MQ=255) acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc > 2:6705705/1‑76 (MQ=255) acaTCCCGGATGCGCCTGTTGCAAAATTTCTTCGTTCGCCGCATTGCACAATACTGCGCGGCCCCCGCGCGCTCGc < 2:342995/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa > 2:6875422/1‑76 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 2:6655199/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 1:1171808/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:2417803/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 1:4310517/76‑1 (MQ=255) ttCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGc < 2:5547730/76‑1 (MQ=255) ttCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGc < 2:2538145/76‑1 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc > 1:3357477/1‑76 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca < 1:1199452/76‑1 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca < 1:2144751/76‑1 (MQ=255) tGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCACCaa > 2:282359/1‑76 (MQ=255) gcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 1:5868177/76‑1 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 2:5707687/1‑76 (MQ=255) ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACg > 2:6010477/1‑76 (MQ=255) ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACg > 2:60533/1‑76 (MQ=255) cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg > 1:1280073/1‑76 (MQ=255) cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg > 2:7253069/1‑76 (MQ=255) gTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGt > 1:2861258/1‑76 (MQ=255) ttGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGtt > 1:6664763/1‑76 (MQ=255) ttGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGtt < 2:2232075/76‑1 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:2669287/1‑76 (MQ=255) gCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCCTCAACACGGGTTCg > 1:2962234/1‑76 (MQ=255) cATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGt > 1:3831425/1‑76 (MQ=255) cATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGt > 2:6680057/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 2:6141043/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 2:4926749/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:7209440/76‑1 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 2:740932/1‑76 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAacac > 2:4322143/1‑66 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 1:609022/1‑76 (MQ=255) aaTTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGAc > 1:3939166/1‑76 (MQ=255) ttcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtc > 1:4777209/1‑76 (MQ=255) ttcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtc > 1:160735/1‑76 (MQ=255) tcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct > 1:4400400/1‑76 (MQ=255) tcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct > 1:5535843/1‑76 (MQ=255) cttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtctc < 2:5461853/76‑1 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa > 1:5060466/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGCCGTCTCAAt > 2:5733989/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt > 2:1702892/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt > 1:6367932/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt < 1:881049/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc < 1:1914126/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTAGCAGCACATCAAGACGGGATCGTGACGTCTCAATc > 2:1743776/1‑76 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCCCTCGCAGCTCATCAACACGGGGTCGTGACGTCTCAATc > 1:4875352/1‑76 (MQ=255) tcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcac > 2:5482266/1‑76 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 2:3162622/1‑76 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACCCGGGTTCGTGACGTCTCAATcacca < 1:1407354/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 1:7717869/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca > 2:1193664/1‑76 (MQ=255) ccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcaccac < 1:5933909/76‑1 (MQ=255) cgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCaccacc > 1:2209930/1‑76 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca > 2:4712966/1‑76 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca < 2:593151/76‑1 (MQ=255) cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg > 1:4785624/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:1863052/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:6924815/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:1496547/1‑76 (MQ=255) ttGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCg < 2:7650233/76‑1 (MQ=255) ttGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCg < 1:3643027/76‑1 (MQ=255) tGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGCTCGTGACGTCTCAATCACCACCAGCgg > 2:5204478/1‑76 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa > 1:2361470/1‑76 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa > 2:1940851/1‑76 (MQ=255) cccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGaa > 1:3262678/1‑76 (MQ=255) cccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGaa < 1:4114995/76‑1 (MQ=255) ccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAat > 2:1975851/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:4685493/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:1538952/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:3217137/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:4782298/1‑76 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACAACCAGCGGAata > 1:3745224/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:5144784/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGCTCGTGACGTCTCAATCACCACCAGCGGAatat > 1:4996704/1‑76 (MQ=255) aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc < 1:4505681/76‑1 (MQ=255) aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc > 2:3732387/1‑76 (MQ=255) tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa > 1:6626122/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag > 2:3275946/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag > 2:399167/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 1:3492627/76‑1 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag > 2:4087476/1‑76 (MQ=255) cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCaga > 2:6179068/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg < 2:6435261/76‑1 (MQ=255) gcgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCg < 1:6252497/76‑1 (MQ=255) gcgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCg < 1:3190843/76‑1 (MQ=255) | CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCG > REL606/473851‑473995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |