| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,389,156 | G→A | V77I (GTA→ATA) | gspG → | pseudopilin, cryptic, general secretion pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,389,156 | 0 | G | A | 98.4% | 711.8 / ‑0.4 | 187 | V77I (GTA→ATA) | gspG | pseudopilin, cryptic, general secretion pathway |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (95/89); total (98/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 239 total aligned reads displayed.
GGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCG > REL606/3389083‑3389228 | gAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtaatag < 1:5985586/76‑1 (MQ=255) aaaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGa < 2:1452152/76‑1 (MQ=255) aaaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGa > 2:1462940/1‑76 (MQ=255) aaaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGa < 1:2461915/76‑1 (MQ=255) aaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATaaaa > 2:5694413/1‑76 (MQ=255) aaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGaa < 1:1303602/76‑1 (MQ=255) aaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGaa > 1:5244153/1‑76 (MQ=255) aaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAg < 1:1138463/76‑1 (MQ=255) aaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAg > 2:4029787/1‑76 (MQ=255) aaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAg > 1:6800872/1‑76 (MQ=255) aCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAgc < 1:1586633/76‑1 (MQ=255) cGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAgcg < 2:4538014/76‑1 (MQ=255) cGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAgcg < 1:2219695/76‑1 (MQ=255) cGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAgcg < 2:6370609/76‑1 (MQ=255) cccTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGcc > 2:1568954/1‑76 (MQ=255) ccTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCg > 1:4362207/1‑76 (MQ=255) cTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGa < 2:322515/76‑1 (MQ=255) atatGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACt > 1:2377055/1‑76 (MQ=255) atatGTACAAACTCGACAATCATCACTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACt < 2:869327/76‑1 (MQ=255) atatGTAAAACCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACt < 2:2403197/76‑1 (MQ=255) atGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGc > 1:5456447/1‑76 (MQ=255) atGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGc < 1:1036118/76‑1 (MQ=255) atGTACAAACTCGACAAGCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGc < 2:102764/76‑1 (MQ=255) tGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGcc > 1:7309878/1‑76 (MQ=255) tGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGcc < 1:1470997/76‑1 (MQ=255) gTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGGCGACACTGCCa > 2:6524725/1‑76 (MQ=255) tACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCAc > 1:3367688/1‑76 (MQ=255) tACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCAc < 1:7105176/76‑1 (MQ=255) aCAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCAcc > 1:481513/1‑76 (MQ=255) aCAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCAcc > 1:3196616/1‑76 (MQ=255) aCAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCAcc < 1:7619448/76‑1 (MQ=255) aaaCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGc > 1:4925808/1‑76 (MQ=255) aCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTg < 1:3633438/76‑1 (MQ=255) cGACAACCATCACTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGcc < 2:3022021/76‑1 (MQ=255) gaaaaTCAGCACTATCCAACCACAAAGCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCg < 2:3735151/73‑1 (MQ=255) aaaaTCATCCCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGc < 2:3506926/74‑1 (MQ=255) cAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCa > 2:2553664/1‑76 (MQ=255) aaTCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCaa > 1:6901558/1‑76 (MQ=255) aaTCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCaa > 1:2797513/1‑76 (MQ=255) aaTCACCACTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCaa < 1:6473209/76‑1 (MQ=255) atcatcACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCaaa > 2:3173946/1‑76 (MQ=255) atcatcACTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCAGCaaa > 2:4623774/1‑76 (MQ=255) atcaacACAACCCAACCCCAAAACGGGGCCTTAAATCCTTAATAGAAGCGCCAACACTGCCACCGCTGGCCGCaaa < 2:6268860/76‑1 (MQ=255) catccCTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt < 1:5270529/76‑1 (MQ=255) catcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt < 1:6653266/76‑1 (MQ=255) catcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt > 1:1423551/1‑76 (MQ=255) catcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt < 1:2984328/76‑1 (MQ=255) catcaCTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt < 1:5512182/76‑1 (MQ=255) atcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACta > 2:2791870/1‑76 (MQ=255) atcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACta > 2:654822/1‑76 (MQ=255) atcaCGACCCAACCACAAATCGGGGGCTTGACTCCTAAATAGAAGCGCCGACCCTGCCACCGCTGGCCGCAAACta < 2:1168101/76‑1 (MQ=255) tcaCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACtat > 2:7366203/1‑76 (MQ=255) ccTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATaa < 1:1098654/75‑1 (MQ=255) ccTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATaa < 1:3995016/75‑1 (MQ=255) aCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATaa > 1:4120488/1‑76 (MQ=255) aCTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATaa > 1:2811107/1‑76 (MQ=255) cTATCCAACCACAAATCAGGGGCTTGAATCCTTAATAGGAGGGCCGACACTGCCACCGCTGGCCGCAAAATATaaa > 2:4090551/1‑75 (MQ=255) cTACCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAAc < 1:4597543/76‑1 (MQ=255) acccAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaa < 2:2949304/74‑1 (MQ=255) aTCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaa > 1:6836948/1‑76 (MQ=255) tCCAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaag > 2:4949255/1‑76 (MQ=255) cccAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaag < 1:6325989/75‑1 (MQ=255) ccAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaagg < 2:5673917/76‑1 (MQ=255) ccAACCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaagg > 1:2542351/1‑76 (MQ=255) acccACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaa < 2:2033001/74‑1 (MQ=255) aaCCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaa > 2:717819/1‑76 (MQ=255) aaCCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaa < 1:6365195/76‑1 (MQ=255) aaCCACAAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaa < 1:6622658/76‑1 (MQ=255) aaCCACAAAGCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaa < 2:4728595/76‑1 (MQ=255) aCCACAAATCAGGGGCTTGAATCCTTAAAAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaag < 2:831758/76‑1 (MQ=255) acaAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGtt < 2:7061265/76‑1 (MQ=255) caAATCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTta < 1:5550549/76‑1 (MQ=255) aaaTCGGGGACTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtat < 2:6156630/76‑1 (MQ=255) aaaTCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtat > 2:2740465/1‑76 (MQ=255) aaaTCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtat < 1:7572268/76‑1 (MQ=255) aaTAAGGGCCTTAAATCTTAAATAAAAGCCCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtata < 2:4309860/76‑1 (MQ=255) aTCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTatat > 2:1891267/1‑76 (MQ=255) taaGGGGGTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATc < 2:5930427/74‑1 (MQ=255) tCAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATc < 1:6548431/76‑1 (MQ=255) ccAGGGGCTGGAGTCCTGAATAGAAGCGCCGACACTGCCCCCGCTGGCCGCAAACTATAACAAGGAAGGTTATATc < 1:6418134/75‑1 (MQ=255) cgggggCTGGAACCCTTAAAAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCa < 1:5011614/74‑1 (MQ=255) cAGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCa > 1:1766709/1‑76 (MQ=255) aGGGGCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCaa > 2:6007823/1‑76 (MQ=255) aGGGGCTTGAATCCTTAATAGAAGAGCCGACACTGCCACCGCTGGTCGCAAACTTTAACAAGGAAGGCTATATCaa > 2:4373833/1‑76 (MQ=255) gggCTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGc < 1:3553345/76‑1 (MQ=255) cTTGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTc < 2:5540661/76‑1 (MQ=255) ttaaaTCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTct < 1:6121971/73‑1 (MQ=255) ttGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTct < 1:2746375/76‑1 (MQ=255) tGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg > 2:6880093/1‑76 (MQ=255) tGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg > 2:2483132/1‑76 (MQ=255) tGAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg < 1:7356670/76‑1 (MQ=255) gAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgc > 2:4848501/1‑76 (MQ=255) gAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgc > 1:4451748/1‑76 (MQ=255) gAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgc > 1:376822/1‑76 (MQ=255) gAATCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgc > 1:5746495/1‑76 (MQ=255) aTCCTTAATAGACGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcct < 2:6585702/76‑1 (MQ=255) aTCCTTAATAAAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcct < 2:5282610/76‑1 (MQ=255) tctttAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcctg < 2:1276599/73‑1 (MQ=255) tCCTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcctg > 2:7520787/1‑76 (MQ=255) cTTAATAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCtgcctgcc > 2:5472134/1‑76 (MQ=255) | GGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCG > REL606/3389083‑3389228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |